S. Sai-Ut

Bio: S. Sai-Ut is an academic researcher. The author has contributed to research in topics: Bromelain (pharmacology) & Protein degradation. The author has an hindex of 2, co-authored 2 publications receiving 73 citations.

Biochemical and functional properties of proteins from red kidney, navy and adzuki beans

Abstract: Chemical composition and functional properties of proteins extracted from adzuki, navy and red kidney beans were investigated. Protein contents were 17.33, 18.15 and 19.91% for red kidney, navy and adzuki beans, respectively. Red kidney bean exhibited the highest carbohydrates content, while the highest fat, ash and crude fibre content was found in navy bean. The highest trypsin inhibitory activity was found in adzuki bean. Globulin polypeptides (molecular weight ranges 45-55 kDa) were the major polypeptides in both whole protein and protein isolate. Protein isolate from all seeds contained at least one major glycopeptide, while more than two major glycopeptides were observed in the whole proteins. The minimum protein solubility of isolated proteins from all seeds was at around pH of 4-5. High foamability and foam stability were obtained in red kidney and navy beans when compared with those of adzuki bean. The highest oil absorption was found in adzuki bean, while no significant differences for water absorption between adzuki and red kidney beans was observed (p>0.05). Red kidney bean showed lower emulsifying activity as well as stability when compared with the other two beans.

Partitioning of bromelain from pineapple peel (Nang Lae cultv.) by aqueous two phase system.

Abstract: Pineapple peel is a potential source for the extraction of beneficial bioactive compounds due to the large amount of waste after processing. The extraction of bromelain from pineapple peel by using aqueous two-phase system (ATPs) and its application in muscle models were investigated. Bromelain predominantly partitions to the polyethylene glycol (PEG) rich top phase. The highest enzyme activity and recovery were presented in the top phase of 15% PEG-3000-20%MgSO4. Protein pattern and activity staining showed the molecular weight (MW) of bromelain was 29 kDa. The extracted bromelain showed the highest activity when incubated with buffer pH 9.0. Its activity decreased by 50% after 5 min incubation at 90°C and dropped drastically after 10 min incubation. Its activity also decreased continuously with increasing of NaCl concentration. Crude bromelain extract was applied to the muscles of beef, giant catfish and squid at the levels 0.05-1.0 grams per two grams of muscle. Higher muscle protein breakdown was observed to occur more in the beef and the giant catfish muscles than that of the squid sample. The myosin heavy chain (MHC) of beef and giant catfish muscles was extensively degraded into degradation proteins with lower MW.

Nutritional and Health Perspectives of Beans (Phaseolus vulgaris L.): An Overview

Abstract: Beans, the variants of Phaseolus vulagris, are nutritionally and economically important food crop in each part of the world. Besides providing nutrients such as multifaceted carbohydrates, elevated proteins, dietary fiber, minerals, and vitamins, these also contain rich variety of polyphenolic compounds with prospective health benefits. This review mainly focuses the important nutritional aspects of beans as well as their contribution in decreasing the risks of chronically degenerative diseases.

Pulse proteins: secondary structure, functionality and applications.

Abstract: Pulses are the second most important source of food for humans after cereals. They hold an important position in human nutrition. They are rich source of proteins, complex carbohydrates, essential vitamins, minerals and phytochemicals and are low in lipids. Pulses are also considered the most suitable for preparing protein ingredients (concentrates and isolates) because of their high protein content, wide acceptability and low cost. In addition, pulse proteins exhibit functional properties (foaming and emulsification, water and fat absorption and gelation) as well as nutraceutical/health benefiting-properties which makes them healthier and low cost alternative to conventional protein sources like soy, wheat and animals. Proteins from different pulses (beans, peas, lentils, cowpeas, chickpeas, pigeon peas, etc.) differ in their composition and structure hence for finished product suitability. Therefore, this article aimed to review composition, structure–function relationship and current applications of different pulse proteins in the food industry.

Aqueous Two-Phase Extraction Advances for Bioseparation

Abstract: Aqueous two-phase extraction (ATPE), unique liquid-liquid extraction, involves a transfer of solute from one aqueous phase to another. ATPE includes polymer–polymer type and polymer–salt type systems for the recovery of proteins. The protein must be recovered in a highly purified form in order to improve its quality, decrease energy consumption, reduce waste and minimize costs. To acquire the high value and achieve good control over processes, the reliable, multi-component products are required especially those with the ability to investigate complex processing conditions. The current reviewing paper discusses the most recent progresses for the recovery of biomolecules by using the ATPE, covering the mechanism, which controls the phase formation and the behavior of solute partitioning in aqueous twophase systems (ATPS) processes. The review discusses also the increasing application for the recovery of high-value bioproducts, the recent development of alternative low cost ATPS and disadvantages attributed to ATPS.