Author
S. Strugger
Bio: S. Strugger is an academic researcher. The author has contributed to research in topics: Fluorescence microscope. The author has an hindex of 1, co-authored 1 publications receiving 115 citations.
Topics: Fluorescence microscope
Papers
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TL;DR: By the staining of soil with the fluorescent dye acridinorange and examination of the stained soil under the fluorescence microscope, it is possible to observe directly the living bacteria of soil in their autochthonic condition.
Abstract: By the staining of soil with the fluorescent dye acridinorange and examination of the stained soil under the fluorescence microscope, it is possible to observe directly the living bacteria of soil ...
116 citations
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TL;DR: The heterotrophic plate count has come under increasing criticism because it is inefficient, at best, for enumerating viable bacteria present in marine and estuarine systems.
1,793 citations
TL;DR: A review of the use of 3,6-bis[dimethylamino]acridinium chloride (acridine orange) and 4',6-diamidino-2-phenylindole (DAPI) stain for direct enumeration of bacteria is presented in this paper.
817 citations
01 Jan 1993
TL;DR: A global systems approach to the Physiology of the Starved Cell and approaches to the Study of Survival and Death in Stationary Phase Escherichia coli K12 are studied.
Abstract: Bioavailability of Energy and the Starvation State (R.Y. Morita). Bacterial Growth and Starvation in Aquatic Environments (D.J.W. Moriarty, R.T. Bell). Bacterial Responses to Soil Stimuli (J.D. Van Elsas, L.S. van Overbeek). Dynamics of Microbial Growth in the Decelerating and Stationary Phases of Batch Culture (C.A. Mason, T. Egli). Starvation and Recovery of Vibrio (J. Ostling et al.). Global Systems Approach to the Physiology of the Starved Cell (T. Nystrom). Approaches to the Study of Survival and Death in Stationary Phase Escherichia coli (D.A. Siegele et al.). The Role of rpoS in Early Stationary Phase Gene Regulation in Escherichia coli K12 (R. HenggeAronis). Gene Expression and Survival during Nutrient Starvation in Salmonella typhimurium (M.P. Spector, J.W. Foster). The Impact of Nutrial State on the Microevolution of Ribosomes (C.G. Kurland, R. Mikkola). Formation of Viable but Nonculturable Cells (J.D. Oliver). Index.
453 citations
01 Jan 1993
TL;DR: It is realized that some bacteria, in response to certain environmental stresses, may lose the ability to grow on media on which they are routinely cultured, while remaining viable, and is of special concern when considering release into the environment of genetically engineered microorganisms and human pathogens.
Abstract: It has long been realized that plate counts can dramatically underestimate the total number of bacteria (typically determined by direct microscopic examination) present in samples taken from natural environments. In the late 1970s, several easily performed noncultural methods (Zimmerman et al., 1978; Kogure et al., 1979) for determining cell viability allowed confirmation of earlier microautoradiographic studies (e.g., Hoppe, 1976) which demonstrated that many of these nonculturable cells are indeed viable and able to actively metabolize. Such studies led to the further realization that some bacteria, in response to certain environmental stresses, may lose the ability to grow on media on which they are routinely cultured, while remaining viable. This state is of considerable interest to our understanding of microbial ecology. It is of special concern when considering release into the environment of genetically engineered microorganisms, and for those indicator bacteria (e.g., coliforms) and human pathogens which enter this nonculturable state and are thus not detectable through routine bacteriological procedures.
355 citations
306 citations