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Sarah H. Hewitt
Researcher at Loughborough University
Publications - 13
Citations - 259
Sarah H. Hewitt is an academic researcher from Loughborough University. The author has contributed to research in topics: Luminescence & Small molecule. The author has an hindex of 7, co-authored 13 publications receiving 179 citations. Previous affiliations of Sarah H. Hewitt include University of Leeds.
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Journal ArticleDOI
Application of lanthanide luminescence in probing enzyme activity
TL;DR: This Feature article focuses on recent progress in the development of enzyme activity assays based on lanthanide(ii) luminescence, covering a variety of strategies including Ln(iii)-labelled antibodies and proteins, LN(iii) ion encapsulation within defined peptide sequences, reactivity-based Ln (iii) probes, and discrete Ln-(iii) complexes.
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Tuning the anion binding properties of lanthanide receptors to discriminate nucleoside phosphates in a sensing array
Sarah H. Hewitt,Georgina Macey,Romain Mailhot,Mark R. J. Elsegood,Fernanda Duarte,Alan M. Kenwright,Stephen J. Butler +6 more
TL;DR: A series of lanthanide-based anion receptors are presented and key structural elements that impact on nucleoside phosphate anion binding and sensing are established and a bidentate (α-, γ-) binding mode to ATP is revealed.
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A continuous luminescence assay for monitoring kinase activity: signalling the ADP/ATP ratio using a discrete europium complex
TL;DR: A stable cationic europium complex binds reversibly to ATP and ADP in water, at neutral pH, in the presence of Mg2+ ions, providing distinctive luminescence responses that permits the kinase-catalysed conversion of ATP to ADP to be monitored in real-time.
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Metal complexes as “protein surface mimetics”
Sarah H. Hewitt,Andrew J. Wilson +1 more
TL;DR: The current state of the art in protein surface recognition is highlighted using metal complexes as surface mimetics using metal based supramolecular scaffolds for protein binding.
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A simple, robust, universal assay for real-time enzyme monitoring by signalling changes in nucleoside phosphate anion concentration using a europium(III)-based anion receptor
Sarah H. Hewitt,Rozee Ali,Romain Mailhot,Chloe R Antonen,Charlotte A. Dodson,Stephen J. Butler +5 more
TL;DR: A simple, sensitive microplate assay for real-time enzyme monitoring, using a lanthanide-based anion receptor, could increase productivity in the drug discovery pipeline.