S
Sayda Elbashir
Researcher at Alnylam Pharmaceuticals
Publications - 71
Citations - 26500
Sayda Elbashir is an academic researcher from Alnylam Pharmaceuticals. The author has contributed to research in topics: RNA silencing & Small interfering RNA. The author has an hindex of 29, co-authored 63 publications receiving 24961 citations. Previous affiliations of Sayda Elbashir include Max Planck Society.
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Journal ArticleDOI
Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells
TL;DR: 21-nucleotide siRNA duplexes provide a new tool for studying gene function in mammalian cells and may eventually be used as gene-specific therapeutics.
Journal ArticleDOI
RNA interference is mediated by 21- and 22-nucleotide RNAs
TL;DR: In this article, the authors demonstrate that 21 and 22-nt RNA fragments are the sequence-specific mediators of RNA interference in a Drosophila in vitro system, and provide evidence that the direction of dsRNA processing determines whether sense or antisense target RNA can be cleaved by the siRNA-protein complex.
Journal ArticleDOI
Therapeutic silencing of an endogenous gene by systemic administration of modified siRNAs
Jürgen Soutschek,Akin Akinc,Birgit Bramlage,Klaus Charisse,Rainer Constien,Mary Donoghue,Sayda Elbashir,Anke Geick,Philipp Hadwiger,Jens Harborth,Matthias John,Venkitasamy Kesavan,Gary Lavine,Rajendra K. Pandey,Timothy Racie,Kallanthottathil G. Rajeev,Ingo Röhl,Ivanka Toudjarska,Gang Wang,Silvio Wuschko,David Bumcrot,Victor Koteliansky,Stefan Limmer,Muthiah Manoharan,Hans-Peter Vornlocher +24 more
TL;DR: In this article, chemically modified short interfering RNAs (siRNAs) were used to silence an endogenous gene encoding apolipoprotein B (apoB) after intravenous injection in mice.
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Functional anatomy of siRNAs for mediating efficient RNAi in Drosophila melanogaster embryo lysate.
TL;DR: Duplexes of 21–23 nucleotide RNAs are the sequence‐specific mediators of RNA interference and post‐transcriptional gene silencing and mismatches in the centre of the siRNA duplex prevent target RNA cleavage, providing a rational basis for the design of siRNAs in future gene targeting experiments.
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Analysis of gene function in somatic mammalian cells using small interfering RNAs
TL;DR: Because of the robustness of the siRNA knockdown technology, genomewide analysis of human gene function in cultured cells has now become possible and here a collection of protocols for siRNA-mediated knockdown of mammalian gene expression is provided.