Sean M. Gross

TL;DR: The LINCS program focuses on cellular physiology shared among tissues and cell types relevant to an array of diseases, including cancer, heart disease, and neurodegenerative disorders.

TL;DR: A fluorescent reporter molecule in a lentiviral delivery system is constructed to assess Akt kinase activity at the single cell level and finds that stimulation of Akt activity by IGF-I is encoded into stable and reproducible analog responses at the population level, but that single cell signaling outcomes are variable.

TL;DR: Using real-time tracking of a fluorescent sensor molecule, these studies address signaling specificity by determining how actions of different growth factors are encoded into distinct Akt signaling dynamics.

TL;DR: Although a relationship at the population level between growth factor-induced Akt signaling dynamics and cell cycle progression could be defined, the fate of individual cells could not be predicted based solely on acute AkT signaling responses, no matter how robust these might be.

TL;DR: The rational development of a genetically encoded FoxO1 sensor is described, which serves as a down-stream readout of insulin growth factor-phosphatidylinositol 3-kinase IGF-PI3K-AKT signaling pathway activity, and these responses are sustained over time, reproducible at the single-cell level, and display cell-to-cell heterogeneity.