S
Sérgio Simões
Researcher at University of Coimbra
Publications - 170
Citations - 8321
Sérgio Simões is an academic researcher from University of Coimbra. The author has contributed to research in topics: Cationic liposome & Gene delivery. The author has an hindex of 46, co-authored 154 publications receiving 7516 citations. Previous affiliations of Sérgio Simões include Bayer & University of the Pacific (United States).
Papers
More filters
Journal ArticleDOI
Paclitaxel-loaded PLGA nanoparticles: preparation, physicochemical characterization and in vitro anti-tumoral activity
TL;DR: The results demonstrate that incorporation of Ptx in nanoparticles strongly enhances the cytotoxic effect of the drug as compared to Taxol, this effect being more relevant for prolonged incubation times.
Journal ArticleDOI
On the formulation of pH-sensitive liposomes with long circulation times
TL;DR: While the incorporation of PEG-lipids reduces the pH-dependent release of encapsulated fluorescent markers in vitro, it does not hinder the cytoplasmic delivery of the markers per cell-associated liposome, suggesting that intracellular delivery is not dictated simply by the destabilization of the liposomes.
Journal ArticleDOI
Cationic lipid-DNA complexes in gene delivery: from biophysics to biological applications.
TL;DR: This review focuses on the various parameters that are considered to be crucial to optimize the use of cationic lipid-DNA complexes for gene therapy purposes, from the biophysical aspects underlying the formation of the complexes to the different biological barriers that need to be surpassed for gene expression to occur.
Journal ArticleDOI
Sterically Stabilized pH-sensitive Liposomes INTRACELLULAR DELIVERY OF AQUEOUS CONTENTS AND PROLONGED CIRCULATION IN VIVO
Vladimir Slepushkin,Sérgio Simões,Paul Dazin,Mary S. Newman,Luke S. Guo,Maria C. Pedroso de Lima,Nejat Düzgüneş,Nejat Düzgüneş +7 more
TL;DR: It is found that addition of PEG-PE to the membrane of pH-sensitive liposomes composed of cholesteryl hemisuccinate and dioleoylphosphatidylethanolamine confers steric stability to these vesicles, which may be useful for the intracellular delivery in vivo of highly negatively charged molecules.
Journal ArticleDOI
Cationic liposomes for gene delivery.
Sérgio Simões,Ana da Silva Filipe,Henrique Faneca,Miguel Mano,Nuno Penacho,Nejat Düzgüneş,Maria C. Pedroso de Lima +6 more
TL;DR: This review will focus on various parameters governing lipoplex biological activity, from their mode of formation to invivo behaviour, in an attempt to dissect the different barriers that need to be surpassed for efficient gene expression to occur.