Author
Seymour. Korkes
Bio: Seymour. Korkes is an academic researcher from New York University. The author has contributed to research in topics: Pyruvate decarboxylation & Carbon fixation. The author has an hindex of 9, co-authored 9 publications receiving 818 citations.
Papers
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209 citations
194 citations
TL;DR: The evidence presented below supports the view that Reaction 1 is a DPN-linked dismutation between Z-malate and pyruvate, resulting from the interaction of lactic dehydrogenase with aDPN-specific “malic” enzyme as shown by Reactions 3 and 4.
112 citations
73 citations
TL;DR: In the absence of orthophosphate, the reaction-rate is sharply reduced and no acetyl phosphate accumulates, and it is possible that the compound may be a coenzyme form of the factor of O'Kane and Gunsalus required for oxidative decarboxylation of pyruvate by S. fæcalis cells.
Abstract: SOLUBLE enzyme preparations from Escherichia coli and Streptococcus faecalis catalyse the dismutation of two molecules of pyruvate to acetyl phosphate, lactate and carbon dioxide when orthophosphate is present. Acetyl phosphate was determined by Lipmann's hydroxylamine reaction1. The system is almost inactive in the absence of added diphosphopyridine nucleotide (DPN). Maximum reaction-rates require, in addition to enzyme, substrate and pyridine nucleotide, the following components: orthophosphate, magnesium ions (Mg++) or manganese ions (Mn++), diphosphothiamine, a boiled extract of yeast, and lactic dehydrogenase. Coenzyme A is required for maximum yields of acetyl phosphate. The nature of the active factor in yeast extract is unknown; but it is not coenzyme A. It is possible that the compound may be a coenzyme form of the factor of O'Kane and Gunsalus2 required for oxidative decarboxylation of pyruvate by S. faecalis cells. In the absence of orthophosphate, the reaction-rate is sharply reduced and no acetyl phosphate accumulates. The dismutation can be formulated as follows:
66 citations
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TL;DR: It is argued that altered metabolism has attained the status of a core hallmark of cancer.
2,623 citations
TL;DR: It is reported that tumor 2HG is elevated in a high percentage of patients with cytogenetically normal acute myeloid leukemia (AML), and AML patients with IDH mutations display a significantly reduced number of other well characterized AML-associated mutations and/or associated chromosomal abnormalities, potentially implicating IDH mutation in a distinct mechanism of AML pathogenesis.
1,790 citations
371 citations
TL;DR: Analysierung des Extraktes von Algen, die in einem stationären Zustand für längere Zeit radioaktive Kohlensäure assimilierten, lieferte weitere Auskunft über den vorgeschlagenen Zyklus and gestattete, die am Zy Klus beteiligten Mengen einiger Substanzen ungefähr z
Abstract: The separation of the phenomenon of photosynthesis in green plants into a photochemical reaction and into the light-dependent reduction of carbon dioxide is discussed, The reduction of carbon dioxide and the fate of the assimilated carbon were investigated with the help of the tracer technique (exposure of the planks to the radioactive C{sup 14}O{sub 2}) and of paper chromatography A reaction cycle is proposed in which phosphoglyceric acid is the first isolable assimilations product Analyses of the algal extracts which had assimilated radioactive carbon dioxide in a stationary condition ('steady-state' photosynthesis) for a long time provided further information concerning the proposed cycle and permitted the approximate estimation, for a number of compounds of what fraction of each compound was taking part in the cycle The earlier supposition that light influences the respiration cycle was confirmed The possibility of the assistance of {alpha}-lipoic acid, or of a related substance, in this influence and in the photosynthesis cycle, is discussed
327 citations