Shaul Reuveny

TL;DR: A review of commercially available microcarriers developed for cultivation of anchorage dependent cells and present current achievements for expansion and differentiation of stem cells is presented in this paper, where key issues such as microcarrier properties and coatings, cell seeding conditions, medium development and improved bioprocess parameters needed for optimal stem cell systems are discussed.

TL;DR: Results clearly demonstrate that neutralizing antibodies to PA constitute a major component of the protective immunity against anthrax and suggest that this parameter could be used as a surrogate marker for protection.

TL;DR: A facile and robust method for maintaining undifferentiated hESC in three-dimensional suspension cultures on matrigel-coated microcarriers achieving 2- to 4-fold higher cell densities than those in 2D colony cultures is developed.

TL;DR: Conventional microcarriers intended for propagation of mammalian cells are not suitable for long term propagation of hESC, so laminin coatedmicrocarriers (DE53 and Cytodex 1) are capable of supporting the longterm propagation and pluripotency of HES-2 and H ES-3 cell lines.

TL;DR: MC cultures are advantageous over MNL cultures in bone tissue engineering because MC-hfMSCs have improved cell expansion capacity and exhibit higher osteogenic potential than MNL-hFMSCs when seeded in vitro into 3D scaffolds or implanted in vivo.