S
Shigeo Nagashima
Researcher at Jichi Medical University
Publications - 98
Citations - 3548
Shigeo Nagashima is an academic researcher from Jichi Medical University. The author has contributed to research in topics: Hepatitis E virus & Hepatitis E. The author has an hindex of 34, co-authored 91 publications receiving 3079 citations. Previous affiliations of Shigeo Nagashima include Sapporo Medical University.
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Journal ArticleDOI
Hepatitis E Virus (HEV) Strains in Serum Samples Can Replicate Efficiently in Cultured Cells Despite the Coexistence of HEV Antibodies: Characterization of HEV Virions in Blood Circulation
Masaharu Takahashi,Toshinori Tanaka,Hideyuki Takahashi,Yu Hoshino,Shigeo Nagashima,Jirintai,Hitoshi Mizuo,Yasuyuki Yazaki,Tomofumi Takagi,Masahiro Azuma,Eiji Kusano,Norio Isoda,Kentaro Sugano,Hiroaki Okamoto +13 more
TL;DR: The results suggest that the in vitro cell culture system developed can be used for propagation of a wide variety of HEV strains in sera from various infected patients, allowing extended studies on viral replication specific to differentHEV strains.
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ORF3 protein of hepatitis E virus is essential for virion release from infected cells.
Kentaro Yamada,Masaharu Takahashi,Yu Hoshino,Hideyuki Takahashi,Koji Ichiyama,Shigeo Nagashima,Toshinori Tanaka,Hiroaki Okamoto +7 more
TL;DR: Results suggest that the ORF3 protein is responsible for virion egress from infected cells and is present on the surface of released HEV particles, which may be associated with lipids.
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Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype.
Masaharu Takahashi,Tsutomu Nishizawa,Hiroyuki Sato,Yukihiro Sato,Jirintai,Shigeo Nagashima,Hiroaki Okamoto +6 more
TL;DR: In this paper, the authors performed a nationwide survey of hepatitis E virus (HEV) infection among 450 wild boars (Sus scrofa leucomystax) that had been captured in Japan between November 2005 and March 2010.
Short Communication Analysis of the full-length genome of a hepatitis E virus isolate obtained from a wild boar in Japan that is classifiable into a novel genotype
Masaharu Takahashi,Tsutomu Nishizawa,Hiroyuki Sato,Yukihiro Sato,Shigeo Nagashima,Hiroaki Okamoto +5 more
TL;DR: The wbJOY_06 isolate is proposed to be the first member of a previously unidentified genotype of hepatitis E virus, which is highly divergent from known genotype 1-4 HEV isolates derived from humans, swine, wild boars, deer, mongoose and rabbits.
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Hepatitis E virus egress depends on the exosomal pathway, with secretory exosomes derived from multivesicular bodies
Shigeo Nagashima,Suljid Jirintai,Masaharu Takahashi,Tominari Kobayashi,Tanggis,Tsutomu Nishizawa,Tom Kouki,Takashi Yashiro,Hiroaki Okamoto +8 more
TL;DR: Findings indicate that membrane-associated HEV particles are released together with internal vesicles through MVBs by the cellular exosomal pathway.