Shinya Kurata

TL;DR: This work focused on the redox properties of some commercially available fluorescent dyes, and investigated dye-nucleotide interactions between a free dye and a nucleotide in aqueous solution by electrochemical and spectroscopic techniques to estimate the fluorescence quenching intensity.

TL;DR: A simple method for the quantitative detection of specific DNA or RNA molecules based on the finding that BODIPY((R)) FL fluorescence was quenched by its interaction with a uniquely positioned guanine.

TL;DR: The bias toward a 1:1 ratio of products in multitemplate PCR used in ecological studies was reevaluated and it was shown that the template reannealing at the annealing step would not cause the bias; however, the preferential homoduplex formation during temperature decrease from denaturation to annealed step wouldcause the bias.

TL;DR: ABC-LAMP enables the accurate quantification of DNA in the presence of DNA amplification inhibitors such as humic acid, urea, and Triton X-100 that compromise the values measured by real-time PCR and real- time turbidimetry of LAMP.

TL;DR: In this article, a method for assaying a nucleic acid comprising the steps of hybridizing the probe consisting of a fluorochrome, 2-O-methyl-oligonucleotide, chimeric oligonucleotide mediated by them to a target n-acid, and so on or assaying the decrease in the fluorescence of the fluoroxide before and after the hybridization, the real-time quantitative PCR method using the method, and a method of analyzing data having a process in which a fluorescence intensity value in the annealing reaction is corrected by that