Shirley Pease

TL;DR: Transgenic mice carrying the green fluorescent protein (GFP) gene driven by a ubiquitously expressing promoter are generated and transgenic rats that express GFP at high levels are generated, suggesting that this technique can be used to produce other transgenic animal species.

TL;DR: A fluorescence decay after photoactivation assay is established to quantitatively study the kinetic behaviour of Oct4, a key transcription factor controlling pre-implantation development in the mouse embryo and identifies Oct4 kinetics, rather than differences in total transcription factor expression levels, as a predictive measure of developmental cell lineage patterning in the early mouse embryo.

TL;DR: This work followed developing T cells at the single-cell level using Bcl11b knock-in fluorescent reporter mice to clarify lineage commitment mechanisms, and identified three distinct, asynchronous mechanisms that act in a stage-specific manner for developmental gene regulation.

TL;DR: Genetic perturbations showed that a distal enhancer controls the rate of epigenetic activation, while a parallel Notch-dependent trans-acting step stimulates expression from activated loci, showing that developmental fate transitions can be controlled by stochastic cis-acting events on individual loci.

TL;DR: This manual provides detailed, step-by-step protocols covering all aspects of the production of transgenic animals, including the use of lentiviral vectors in gene transfer, intracytoplasmic sperm injection, nuclear transfer, large insert transgenesis, conditional gene expression systems, and transGenesis in large animals and birds.