https://www.tpbin.com/Uploads/Subjects/dcb21eff-f04d-459d-bc06-e9798efc66c7.pdf

Collagen for bone tissue regeneration.

With an ageing population, war, and sports related injuries there is an ever-expanding requirement for hard tissue replacement such as bone. Engineered artificial scaffold biomaterials with appropriate mechanical properties, surface chemistry and surface topography are in a great demand for enhancing cell attachment, cell growth and tissue formation at such defect sites. Most of these engineering techniques are aimed at mimicking the natural organization of the bone tissues and thereby create a conducive environment for bone regeneration. As the interaction between the cells and tissues with biomaterials at the tissue–implant interface is a surface phenomenon, surface properties play a major role in determining both the biological response to implants and the material response to the physiological condition. Hence surface engineering of biomaterials is aimed at modifying the material and biological responses through changes in surface properties while still maintaining the bulk mechanical properties of the implant. Therefore, there has been a great thrust towards development of Ca–P-based surface coatings on various metallic and nonmetallic substrates for load bearing implant applications such as hip joint prosthesis, knee joint prosthesis and dental implants. Typical coating methodologies like ion beam assisted deposition, plasma spray deposition, pulsed laser physical vapor deposition, magnetron sputtering, sol–gel derived coatings, electrodeposition, micro-arc oxidation and laser deposition are extensively studied at laboratory scale. In the present article, attempts are made to give an overview of the basic principles behind the coating techniques as well as advantageous features such as bioactivity and biocompatibility associated with these coatings. A strong emphasis will be given on laser-induced textured and bioactive coatings obtained by the author's research group [A. Kurella, N.B. Dahotre, Journal of Biomedical Applications 20 (2005) 5–50; A. Kurella, N.B. Dahotre, Acta Biomaterialia 2 (2006) 677–688; A. Kurella, N.B. Dahotre, Journal of Minerals, Metals and Materials Society (JOM) 58 (2006) 64–66; A. Kurella, N.B. Dahotre, Journal of Materials Science: Materials in Medicine 17 (2006) 565–572; P.G. Engleman, A. Kurella, A. Samant, C.A. Blue, N.B. Dahotre, Journal of Minerals, Metals and Materials Society (JOM) 57 (2005) 46–50; R. Singh, A. Kurella, N.B. Dahotre, Journal of Biomaterials Applications 21 (2006) 46–72; S.R. Paital, N.B. Dahotre, Biomedical Materials 2 (2007) 274–281; S.R. Paital, N.B. Dahotre, 2009, Acta Biomaterialia, doi:10.1016/j.actbio.2009.03.004 ; R. Singh, N.B. Dahotre, Journal of Materials Science: Materials in Medicine 18 (2007) 725–751.]. Since cells are sensitive to topographical features ranging from mesoscale to nanoscale, formation of these features by both pulsed and continuous wave Nd:YAG laser system will be highlighted. This can also be regarded as advancement towards third generation biomaterials which are bioinert, bioactive and which once implanted will stimulate cell adhesion, proliferation and growth at the interface. Further, an overview of various bio-implants and bio-devices and materials used for these kinds of devices, performance factors such as mechanical and corrosion behavior and surface science associated with these materials are also explained. As the present article is aimed at describing the multidisciplinary nature of this exciting field it also provides a common platform to understand this subject in a simple way for students, researchers, teachers and engineers in the fields ranging from medicine, dentistry, biology, materials science, biomedicine, biomechanics to physics.

Calcium phosphate coatings for bio-implant applications: Materials, performance factors, and methodologies

Calcium phosphate coatings on magnesium alloys for biomedical applications: a review.

Guided bone regeneration (GBR) is commonly used in combination with the installment of titanium implants. The application of a membrane to exclude non-osteogenic tissues from interfering with bone regeneration is a key principle of GBR. Membrane materials possess a number of properties which are amenable to modification. A large number of membranes have been introduced for experimental and clinical verification. This prompts the need for an update on membrane properties and the biological outcomes, as well as a critical assessment of the biological mechanisms governing bone regeneration in defects covered by membranes. The relevant literature for this narrative review was assessed after a MEDLINE/PubMed database search. Experimental data suggest that different modifications of the physicochemical and mechanical properties of membranes may promote bone regeneration. Nevertheless, the precise role of membrane porosities for the barrier function of GBR membranes still awaits elucidation. Novel experimental findings also suggest an active role of the membrane compartment per se in promoting the regenerative processes in the underlying defect during GBR, instead of being purely a passive barrier. The optimization of membrane materials by systematically addressing both the barrier and the bioactive properties is an important strategy in this field of research.

https://onlinelibrary.wiley.com/doi/pdf/10.1111/eos.12364

Guided bone regeneration: materials and biological mechanisms revisited.

Texture modification has become one of the most common forms of intervention for dysphagia, and is widely considered important for promoting safe and efficient swallowing. However, to date, there is no single convention with respect to the terminology used to describe levels of liquid thickening or food texture modification for clinical use. As a first step toward building a common taxonomy, a systematic review was undertaken to identify empirical evidence describing the impact of liquid consistency and food texture on swallowing behavior. A multi-engine search yielded 10,147 non-duplicate articles, which were screened for relevance. A team of ten international researchers collaborated to conduct full-text reviews for 488 of these articles, which met the study inclusion criteria. Of these, 36 articles were found to contain specific information comparing oral processing or swallowing behaviors for at least two liquid consistencies or food textures. Qualitative synthesis revealed two key trends with respect to the impact of thickening liquids on swallowing: thicker liquids reduce the risk of penetration–aspiration, but also increase the risk of post-swallow residue in the pharynx. The literature was insufficient to support the delineation of specific viscosity boundaries or other quantifiable material properties related to these clinical outcomes. With respect to food texture, the literature pointed to properties of hardness, cohesiveness, and slipperiness as being relevant both for physiological behaviors and bolus flow patterns. The literature suggests a need to classify food and fluid behavior in the context of the physiological processes involved in oral transport and flow initiation.

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The Influence of Food Texture and Liquid Consistency Modification on Swallowing Physiology and Function: A Systematic Review

Objectives: Although bone loss around implants is reported as a complication when it progresses uncontrolled, resorption does not always lead to implant loss, but may be the result of biomechanical adaptation to stress. To verify this hypothesis, a three-dimensional finite element analysis was performed and the influence of marginal bone resorption amount and shape on stress in the bone and implant was investigated.

Material and methods: A total of nine bone models with an implant were created: a non-resorption (Base) model and eight variations, in which three different resorption depths were combined with pure vertical or conical (vertical–horizontal) resorption. Axial and buccolingual forces were applied independently to the occlusal node at the center of the superstructure.

Results: Regardless of load direction, bone stresses were higher in the pure vertical resorption (A) models than in the Base model, and increased with resorption depth. However, cortical bone stress was much lower in the conical resorption models than in both the Base and A models of the same resorption depth. An opposite tendency was observed in the cancellous bone under buccolingual load.

Under buccolingual load, highest stress in the implant increased linearly with the resorption depth for all the models and its location approached the void existing below the abutment screw.

Conclusions: The results of this analysis suggest that a certain amount of conical resorption may be the result of biomechanical adaptation of bone to stress. However, as bone resorption progresses, the increasing stresses in the cancellous bone and implant under lateral load may result in implant failure.

Biomechanical aspects of marginal bone resorption around osseointegrated implants: considerations based on a three-dimensional finite element analysis.

Statement of Problem. Because of reported mechanical failures, alternative implant system components with suggested optimized strength have been manufactured. However, the endurance of these products has not been well investigated. Purpose. This study was designed to assess the effect of joint design on the fatigue strength and failure mode of 2 single-tooth implant systems: Branemark and ITI, in which a hex mediated-butt joint and 8-degree internal conical implant/abutment interface are used, respectively. Material and Methods. Seven 10-mm implants from each implant system were embedded to a depth of 7 mm in cylindrical acrylic resin blocks. CeraOne and Solid abutments with cement-retained castings were assembled to the Branemark and ITI implants, respectively. The assembled units were mounted in a lever-type-testing machine that was equipped with an automatic counting device and shutoff sensors, enabling the recording of the number of cycles till failure. A cyclic load of 100 N was applied perpendicular to the long axis of the assemblies at a rate of 75 cycles/min. To investigate specimen resistance to fatigue during 6 years of simulated function, a target of 1,800,000 cycles was defined. Specimen preparation and testing was performed by the same operator. The association of the joint design with the occurrence of failure was verified by Fisher's exact probability test ( P Results. For the Branemark group, the gold alloy abutment screw in all specimens fractured between 1,178,023 and 1,733,526 cycles with a standard deviation of 224,477 cycles. For the ITI group, all specimens had no failure until 1,800,000 cycles. Statistical analysis showed a highly significant difference between the 2 groups ( P =.000582). Conclusion. Within the limitations of this in vitro study, the effect of joint design on the fatigue strength and failure mode of the ITI single tooth implant system was significantly better ( P >.001) than the Branemark single-tooth implant system tested. (J Prosthet Dent 2002;88:604-10.)

Fatigue resistance of two implant/abutment joint designs.

Statement of problem Efforts to reduce the recurrence of abutment screw loosening with single tooth implant-supported restorations have been reported. However, the current knowledge about the role of the implant external hexagon is incomplete. Purpose This in vitro study investigated the effect of lateral cyclic loading with different load positions on abutment screw loosening of an external hexagon implant system. Material and methods Fifteen Branemark implant assemblies were divided equally into 3 groups, A, B, and C. Each assembly consisted of a Mark IV implant (4 × 10 mm) mounted in a brass block, a CeraOne abutment (3 mm), and an experimental cement-retained superstructure. For group A, a cyclic load of 50 N was applied centrally and perpendicular to the long axis of the implant, whereas for group B, the same load was applied eccentrically (at a distance of 4 mm) in a loosening direction. A target of 1.0×10 6 cycles (40 months of simulated function) was defined. Group C (control) was left unloaded for the same loading time period as groups A and B. Reverse torque was recorded before and after loading and the difference was calculated. The data were analyzed with 1-way analysis of variance and compared with the Tukey test (α=.05). Results Group A exhibited a significant difference in the reverse torque difference values ([−5.6 to −3.4] ± 0.86 N·cm) compared with groups B ([−1.9 to 0.5] ± 0.99 N·cm) and C ([−0.7 to 0.0] ± 0.26 N·cm) ( P Conclusion Within the limitations of this study, reverse torque values of the screw joint were preserved under eccentric lateral loading, as compared with centric loading ( P

Effect of lateral cyclic loading on abutment screw loosening of an external hexagon implant system

This study was designed to investigate the responses of bone cells to a deproteinized bovine bone material, Bio-Oss (Geistlich-Pharma, Wolhunsen, Switzerland), which was grafted in artificial bone defects of rat femurs. Standardized bone defects in the cortical bone of the right femurs were grafted with Bio-Oss particles. Narrow penetrations were prepared on the bottom of the cavity, enabling osteogenic cells to migrate from the bone marrow. A defect in the left femur without Bio-Oss was used as a control. The treated femurs were histochemically examined at 1, 3, 5, 7, and 14 days after the operation. At day 1, no osteogenic migration into the cavities occurred in either the control or experimental groups. At day 3, alkaline phosphatase (ALPase) immunohistochemistry showed a migration of the positive cells at the bottom of the cavities of the experimental groups, but not in the control ones. At day 5, new bone formation was recognized at the bottom of the cavity of both groups. In the experimental group, ALPase-positive cells were localized on Bio-Oss and/or on the thin bone matrix that covered this material. The superficial layer of Bio-Oss underlying the newly formed bone exhibited osteocalcin immunoreactivity. Transmission electron microscopy revealed osteoblasts depositing bone matrices--including collagen fibers--on the surface of Bio-Oss. At days 7 and 14, woven bone occupied the previous cavities of both control and experimental groups, accompanied by osteoclasts. Thus, Bio-Oss appears to serve as a scaffold for osteogenic cells as well as to promote osteoblastic differentiation and matrix synthesis.

Shuichi Nomura

Papers

Biomechanical aspects of marginal bone resorption around osseointegrated implants: considerations based on a three-dimensional finite element analysis.

Fatigue resistance of two implant/abutment joint designs.

Effect of lateral cyclic loading on abutment screw loosening of an external hexagon implant system

A histological evaluation of the involvement of Bio‐Oss® in osteoblastic differentiation and matrix synthesis

A histological evaluation for guided bone regeneration induced by a collagenous membrane