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Shuo Chen

Bio: Shuo Chen is an academic researcher from Northeastern University (China). The author has contributed to research in topics: Medicine & Odontoblast. The author has an hindex of 36, co-authored 221 publications receiving 5208 citations. Previous affiliations of Shuo Chen include Fourth Military Medical University & Chinese Ministry of Education.


Papers
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Journal ArticleDOI
TL;DR: The PDL contains SC that have the potential to differentiate into osteoblasts, chondrocytes and adipocytes, comparable with previously characterized BMSC, and can be utilized for potential therapeutic procedures related to PDL regeneration.
Abstract: Structured Abstract Authors – Gay IC, Chen S, MacDougall M Backround – Periodontal ligament (PDL) repair is thought to involve mesenchymal progenitor cells capable of forming fibroblasts, osteoblasts and cementoblasts. However, full characterization of PDL stem cell (SC) populations has not been achieved. Objective – To isolate and characterize PDLSC and assess their capability to differentiate into bone, cartilage and adipose tissue. Methods – Human PDL cells were stained for STRO-1, FACS sorted and expanded in culture. Human bone marrow SC (BMSC) served as a positive control. PDLSC and BMSC were cultured using standard conditions conducive for osteogenic, chondrogenic and adipogenic differentiation. Osteogenic induction was assayed using alizarine red S staining and expression of alkaline phosphatase (ALP) and bone sialoprotein (BSP). Adipogenic induction was assayed using Oil Red O staining and the expression of PPARγ 2 (early) and LPL (late) adipogenic markers. Chondrogenic induction was assayed by collagen type II expression and toluidine blue staining. Results – Human PDL tissue contains about 27% STRO-1 positive cells with 3% strongly positive. In osteogenic cultures ALP was observed by day-7 in BMSC and day-14 in PDLSC. BSP expression was detectable by day-7; with more intense staining in PDLSC cultures. In adipogenic cultures both cell populations showed positive Oil Red O staining by day-25 with PPARγ 2 and LPL expression. By day-21, both BMSC and PDLSC chondrogenic induced cultures expressed collagen type II and glycosaminoglycans. Conclusions – The PDL contains SC that have the potential to differentiate into osteoblasts, chondrocytes and adipocytes, comparable with previously characterized BMSC. This adult PDLSC population can be utilized for potential therapeutic procedures related to PDL regeneration.

383 citations

Book ChapterDOI
TL;DR: This chapter describes the different aspects of regulation of androgen action, a member of the steroid-thyroid hormone-retinoid-vitamin D superfamily of nuclear receptors (NRs) that function as ligand-activated transcription factors.
Abstract: Publisher Summary This chapter describes the different aspects of regulation of androgen action. Androgens belong to a class of C-19 steroids secreted primarily by the testis and adrenal cortex. Hormonally active androgens promote reproductive and anabolic functions. Both reproductive and anabolic effects of androgens are mediated by their interaction with the androgen receptor (AR), a member of the steroid-thyroid hormone-retinoid-vitamin D superfamily of nuclear receptors (NRs) that function as ligand-activated transcription factors. Almost all of the androgen functions, except its conversion to estrogen by the enzyme aromatase in certain target cells are known to be mediated by the androgen receptor. The androgen receptor are coded by a single copy gene, which is located on the X chromosome. The functional relevance of the segmented domain structure of the NR superfamily is supported by the results of deletion mutagenesis and domain swapping among various receptor proteins. The interaction between the amino terminal and the steroid-binding carboxy-terminal end in the AR transactivation function was initially indicated by the finding that a segment within the hormone-binding domain exerts an inhibitory influence in the transcription regulatory activity of the AR, and the deletion of this region results in ligand-independent activation of the receptor.

288 citations

Journal ArticleDOI
TL;DR: A conceptual smart pre-copy live migration approach is presented for VM migration that can estimate the downtime after each iteration to determine whether to proceed to the stop-and-copy stage during a system failure or an attack on a fog computing node.
Abstract: Fog computing, an extension of cloud computing services to the edge of the network to decrease latency and network congestion, is a relatively recent research trend. Although both cloud and fog offer similar resources and services, the latter is characterized by low latency with a wider spread and geographically distributed nodes to support mobility and real-time interaction. In this paper, we describe the fog computing architecture and review its different services and applications. We then discuss security and privacy issues in fog computing, focusing on service and resource availability. Virtualization is a vital technology in both fog and cloud computing that enables virtual machines (VMs) to coexist in a physical server (host) to share resources. These VMs could be subject to malicious attacks or the physical server hosting it could experience system failure, both of which result in unavailability of services and resources. Therefore, a conceptual smart pre-copy live migration approach is presented for VM migration. Using this approach, we can estimate the downtime after each iteration to determine whether to proceed to the stop-and-copy stage during a system failure or an attack on a fog computing node. This will minimize both the downtime and the migration time to guarantee resource and service availability to the end users of fog computing. Last, future research directions are outlined.

257 citations

Journal ArticleDOI
TL;DR: Analysis of data suggests differential biological functions of Runx2, Osx, and Dspp during odontogenesis and osteogenesis.
Abstract: The transcription factors Runx2 and Osx are necessary for osteoblast and odontoblast differentiation, while Dspp is important for odontoblast differentiation. The relationship among Runx2, Osx, and Dspp during tooth and craniofacial bone development remains unknown. In this study, we hypothesized that the roles of Runx2 and Osx in the regulation of osteoblast and odontoblast lineages may be independent of one another. The results showed that Runx2 expression overlapped with Osx in dental and osteogenic mesenchyme from E12 to E16. At the later stages, from E18 to PN14, Runx2 and Osx expressions remained intense in alveolar bone osteoblasts. However, Runx2 expression was down-regulated, whereas Osx expression was clearly seen in odontoblasts. At later stages, Dspp transcription was weakly present in osteoblasts, but strong in odontoblasts where Osx was highly expressed. In mouse odontoblast-like cells, Osx overexpression increased Dspp transcription. Analysis of these data suggests differential biological functions of Runx2, Osx, and Dspp during odontogenesis and osteogenesis. Abbreviations: E, embryonic day; PN, post-natal day; Dspp, dentin sialophosphoprotein; Osx, Osterix.

218 citations

Journal ArticleDOI
TL;DR: Raman spectroscopy is an important and powerful technique for analyzing the chemical composition of biological or nonbiological samples in many fields as mentioned in this paper, and several different techniques have been explored for this purpose.
Abstract: Raman spectroscopy is an important and powerful technique for analyzing the chemical composition of biological or nonbiological samples in many fields. A serious challenge frequently encountered in Raman measurements arises from the existence of the concurrent fluorescence background. The fluorescence intensity is normally several orders of magnitude larger than the Raman scattering signal, especially in biological samples. Such fluorescence background must be suppressed in order to obtain accurate Raman spectra. Several different techniques have been explored for this purpose. These techniques could be generally grouped into time-domain, frequency-domain, wavelength-domain, and computational methods in addition to various Raman enhancement techniques and other unconventional methods. This review briefly describes the fundamental principles of each group of methods, reports the most recent advances, and makes comparison across those major categories of techniques in terms of cost and performance i...

190 citations


Cited by
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Journal Article
TL;DR: Research data show that more resistant stem cells than common cancer cells exist in cancer patients, and to identify unrecognized differences between cancer stem cells and cancer cells might be able to develop effective classification, diagnose and treat for cancer.
Abstract: Stem cells are defined as cells able to both extensively self-renew and differentiate into progenitors. Research data show that more resistant stem cells than common cancer cells exist in cancer patients.To identify unrecognized differences between cancer stem cells and cancer cells might be able to develope effective classification,diagnose and treat ment for cancer.

2,194 citations

Journal ArticleDOI
24 Dec 2004-Science

1,949 citations

Journal ArticleDOI
TL;DR: This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs.
Abstract: To date, 5 different human dental stem/progenitor cells have been isolated and characterized: dental pulp stem cells (DPSCs), stem cells from exfoliated deciduous teeth (SHED), periodontal ligament stem cells (PDLSCs), stem cells from apical papilla (SCAP), and dental follicle progenitor cells (DFPCs). These post-natal populations have mesenchymal-stem-cell-like (MSC) qualities, including the capacity for self-renewal and multilineage differentiation potential. MSCs derived from bone marrow (BMMSCs) are capable of giving rise to various lineages of cells, such as osteogenic, chondrogenic, adipogenic, myogenic, and neurogenic cells. The dental-tissue-derived stem cells are isolated from specialized tissue with potent capacities to differentiate into odontogenic cells. However, they also have the ability to give rise to other cell lineages similar to, but different in potency from, that of BMMSCs. This article will review the isolation and characterization of the properties of different dental MSC-like populations in comparison with those of other MSCs, such as BMMSCs. Important issues in stem cell biology, such as stem cell niche, homing, and immunoregulation, will also be discussed.

1,643 citations