Sibylle Häggqvist

Bio: Sibylle Häggqvist is an academic researcher from Swedish Museum of Natural History. The author has contributed to research in topics: Biodiversity & Monophyly. The author has an hindex of 5, co-authored 6 publications receiving 49 citations. Previous affiliations of Sibylle Häggqvist include Stockholm University.

Biodiversity assessments in the 21st century: The potential of insect traps to complement environmental samples for estimating eukaryotic and prokaryotic diversity using high-throughput DNA metabarcoding.

Abstract: The rapid loss of biodiversity, coupled with difficulties in species identification, call for innovative approaches to assess biodiversity. Insects make up a substantial proportion of extant divers...

Completing Linnaeus’s inventory of the Swedish insect fauna: Only 5,000 species left?

Abstract: Despite more than 250 years of taxonomic research, we still have only a vague idea about the true size and composition of the faunas and floras of the planet. Many biodiversity inventories provide limited insight because they focus on a small taxonomic subsample or a tiny geographic area. Here, we report on the size and composition of the Swedish insect fauna, thought to represent roughly half of the diversity of multicellular life in one of the largest European countries. Our results are based on more than a decade of data from the Swedish Taxonomy Initiative and its massive inventory of the country's insect fauna, the Swedish Malaise Trap Project The fauna is considered one of the best known in the world, but the initiative has nevertheless revealed a surprising amount of hidden diversity: more than 3,000 new species (301 new to science) have been documented so far. Here, we use three independent methods to analyze the true size and composition of the fauna at the family or subfamily level: (1) assessments by experts who have been working on the most poorly known groups in the fauna; (2) estimates based on the proportion of new species discovered in the Malaise trap inventory; and (3) extrapolations based on species abundance and incidence data from the inventory. For the last method, we develop a new estimator, the combined non-parametric estimator, which we show is less sensitive to poor coverage of the species pool than other popular estimators. The three methods converge on similar estimates of the size and composition of the fauna, suggesting that it comprises around 33,000 species. Of those, 8,600 (26%) were unknown at the start of the inventory and 5,000 (15%) still await discovery. We analyze the taxonomic and ecological composition of the estimated fauna, and show that most of the new species belong to Hymenoptera and Diptera groups that are decomposers or parasitoids. Thus, current knowledge of the Swedish insect fauna is strongly biased taxonomically and ecologically, and we show that similar but even stronger biases have distorted our understanding of the fauna in the past. We analyze latitudinal gradients in the size and composition of known European insect faunas and show that several of the patterns contradict the Swedish data, presumably due to similar knowledge biases. Addressing these biases is critical in understanding insect biomes and the ecosystem services they provide. Our results emphasize the need to broaden the taxonomic scope of current insect monitoring efforts, a task that is all the more urgent as recent studies indicate a possible worldwide decline in insect faunas.

Completing Linneaus's inventory of the Swedish insect fauna: only 5,000 species left?

Abstract: Despite more than 250 years of taxonomic research, we still have only a vague idea about the true size and composition of the faunas and floras of the planet. Many biodiversity inventories provide limited insight because they focus on a small taxonomic subsample or a tiny geographic area. Here, we report on the size and composition of the Swedish insect fauna, thought to represent roughly half of the diversity of multicellular life in one of the largest European countries. Our results are based on more than a decade of data from the Swedish Taxonomy Initiative and its massive inventory of the country9s insect fauna, the Swedish Malaise Trap Project The fauna is considered one of the best known in the world, but the initiative has nevertheless revealed a surprising amount of hidden diversity: more than 3,000 new species (301 new to science) have been documented so far. Here, we use three independent methods to analyze the true size and composition of the fauna at the family or subfamily level: (1) assessments by experts who have been working on the most poorly known groups in the fauna; (2) estimates based on the proportion of new species discovered in the Malaise trap inventory; and (3) extrapolations based on species abundance and incidence data from the inventory. For the last method, we develop a new estimator, the combined non-parametric estimator, which we show is less sensitive to poor coverage of the species pool than other popular estimators. The three methods converge on similar estimates of the size and composition of the fauna, suggesting that it comprises around 33,000 species. Of those, 8,600 (26%) were unknown at the start of the inventory and 5,000 (15%) still await discovery. We analyze the taxonomic and ecological composition of the estimated fauna, and show that most of the new species belong to Hymenoptera and Diptera groups that are decomposers or parasitoids. Thus, current knowledge of the Swedish insect fauna is strongly biased taxonomically and ecologically, and we show that similar but even stronger biases have distorted our understanding of the fauna in the past. We analyze latitudinal gradients in the size and composition of known European insect faunas and show that several of the patterns contradict the Swedish data, presumably due to similar knowledge biases. Addressing these biases is critical in understanding insect biomes and the ecosystem services they provide. Our results emphasize the need to broaden the taxonomic scope of current insect monitoring efforts, a task that is all the more urgent as recent studies indicate a possible worldwide decline in insect faunas.

A new species group in Megaselia, the lucifrons group, with description of a new species (Diptera, Phoridae)

Abstract: With 1,400 described species, Megaselia is one of the most species-rich genera in the animal kingdom, and at the same time one of the least studied. An important obstacle to taxonomic progress is the lack of knowledge concerning the phylogenetic structure within the genus. Classification of Megaselia at the level of subgenus is incomplete although Schmitz addressed several groups of species in a series of monographs published from 1956 to 1981. Another problem is the lack of molecular phylogenetic analyses to support morphology-based conclusions. As a contribution towards addressing these problems, we here circumscribe a previously unrecognized monophyletic lineage of Megaselia consisting of species similar to Megaselialucifrons. We base this taxonomic decision on morphological study of an extensive phorid material from Sweden, complemented by molecular analyses of select exemplars using two markers (COI and 28S). We name the clade the lucifrons species group, and show that it contains three distinct species. Our results also demonstrate that Megaseliasubnitida Lundbeck, 1920, previously treated as a synonym of Megaselialucifrons Schmitz, 1918, is a separate species, and we remove it from synonymy. The third species in the group was previously unknown; we describe it here as Megaseliaalbalucifrons sp. n.

Scuttling towards monophyly: phylogeny of the mega‐diverse genus Megaselia (Diptera: Phoridae)

Abstract: The genus Megaselia Rondani (Diptera: Phoridae) is one of the largest in the animal kingdom, with nearly 1700 described species and many remaining to be discovered. Work on this group is notoriously challenging due to the extreme species diversity, poor knowledge of higher‐level relationships and lack of molecular data. In this paper, we present the largest study to date of Megaselia relationships based on molecular data from one nuclear (28S rDNA) and three mitochondrial (ND1, COI and 16S) markers for 175 Nordic specimens representing 145 species of Megaselia, plus outgroups. Based on phylogenetic analyses of these data, we propose 22 informal Megaselia species groups, all of which match well‐supported terminal clades. Relationships among these groups, and between them and several isolated species, remain largely uncertain. Of the 22, 20 species groups fall into a moderately well‐supported monophyletic clade of ‘core Megaselia’. Two species groups, the spinigera and ruficornis groups, fall outside of core Megaselia, as does the single representative of Myriophora, a genus that is included in Megaselia by some specialists. Here, we explore the morphology of these molecular species groups to aid future studies, and we discuss the implications of our findings for the generic circumscription of Megaselia. Hopefully, our results can aid further characterization of subgroups within the enormous Megaselia radiation and among its closest relatives, thus facilitating future work on this challenging but fascinating group of small flies.

Ten golden rules for reforestation to optimize carbon sequestration, biodiversity recovery and livelihood benefits

Abstract: Urgent solutions to global climate change are needed. Ambitious tree-planting initiatives, many already underway, aim to sequester enormous quantities of carbon to partly compensate for anthropogenic CO2 emissions, which are a major cause of rising global temperatures. However, tree planting that is poorly planned and executed could actually increase CO2 emissions and have long-term, deleterious impacts on biodiversity, landscapes and livelihoods. Here, we highlight the main environmental risks of large-scale tree planting and propose 10 golden rules, based on some of the most recent ecological research, to implement forest ecosystem restoration that maximizes rates of both carbon sequestration and biodiversity recovery while improving livelihoods. These are as follows: (1) Protect existing forest first; (2) Work together (involving all stakeholders); (3) Aim to maximize biodiversity recovery to meet multiple goals; (4) Select appropriate areas for restoration; (5) Use natural regeneration wherever possible; (6) Select species to maximize biodiversity; (7) Use resilient plant material (with appropriate genetic variability and provenance); (8) Plan ahead for infrastructure, capacity and seed supply; (9) Learn by doing (using an adaptive management approach); and (10) Make it pay (ensuring the economic sustainability of the project). We focus on the design of long-term strategies to tackle the climate and biodiversity crises and support livelihood needs. We emphasize the role of local communities as sources of indigenous knowledge, and the benefits they could derive from successful reforestation that restores ecosystem functioning and delivers a diverse range of forest products and services. While there is no simple and universal recipe for forest restoration, it is crucial to build upon the currently growing public and private interest in this topic, to ensure interventions provide effective, long-term carbon sinks and maximize benefits for biodiversity and people.

The pitfalls of biodiversity proxies: Differences in richness patterns of birds, trees and understudied diversity across Amazonia

Abstract: Most knowledge on biodiversity derives from the study of charismatic macro-organisms, such as birds and trees. However, the diversity of micro-organisms constitutes the majority of all life forms on Earth. Here, we ask if the patterns of richness inferred for macro-organisms are similar for micro-organisms. For this, we barcoded samples of soil, litter and insects from four localities on a west-to-east transect across Amazonia. We quantified richness as Operational Taxonomic Units (OTUs) in those samples using three molecular markers. We then compared OTU richness with species richness of two relatively well-studied organism groups in Amazonia: trees and birds. We find that OTU richness shows a declining west-to-east diversity gradient that is in agreement with the species richness patterns documented here and previously for birds and trees. These results suggest that most taxonomic groups respond to the same overall diversity gradients at large spatial scales. However, our results show a different pattern of richness in relation to habitat types, suggesting that the idiosyncrasies of each taxonomic group and peculiarities of the local environment frequently override large-scale diversity gradients. Our findings caution against using the diversity distribution of one taxonomic group as an indication of patterns of richness across all groups.

Prospects and challenges of implementing DNA metabarcoding for high-throughput insect surveillance

Abstract: Trap-based surveillance strategies are widely used for monitoring of invasive insect species, aiming to detect newly arrived exotic taxa as well as track the population levels of established or endemic pests. Where these surveillance traps have low specificity and capture non-target endemic species in excess of the target pests, the need for extensive specimen sorting and identification creates a major diagnostic bottleneck. While the recent development of standardized molecular diagnostics has partly alleviated this requirement, the single specimen per reaction nature of these methods does not readily scale to the sheer number of insects trapped in surveillance programmes. Consequently, target lists are often restricted to a few high-priority pests, allowing unanticipated species to avoid detection and potentially establish populations. DNA metabarcoding has recently emerged as a method for conducting simultaneous, multi-species identification of complex mixed communities and may lend itself ideally to rapid diagnostics of bulk insect trap samples. Moreover, the high-throughput nature of recent sequencing platforms could enable the multiplexing of hundreds of diverse trap samples on a single flow cell, thereby providing the means to dramatically scale up insect surveillance in terms of both the quantity of traps that can be processed concurrently and number of pest species that can be targeted. In this review of the metabarcoding literature, we explore how DNA metabarcoding could be tailored to the detection of invasive insects in a surveillance context and highlight the unique technical and regulatory challenges that must be considered when implementing high-throughput sequencing technologies into sensitive diagnostic applications.

FLIES - The Natural History and Diversity of Diptera

Abstract: Human knowledge will be erased from the world’s archives before we possess the last word that a gnat has to say to us. -Henri Fabre- The new book, FLIES, tells us a lot about what gnats and their relatives have to tell us about nature, would we but listen attentively. The author, Stephen Marshall, began his biological interests in beetles and then somehow went to the dark side, FLIES. This reviewer began his career in beetles and stayed put; that is what qualifies him as a reviewer of this simply superb book Steve Marshall has produced both in text and images. Why? It’s in the subtitle: the Natural History and Diversity of Diptera, the key word being diversity by which Marshall means many different kinds. Beetles and parasitic wasps are universally touted as the hyper-diverse and divergent groups of insects, so why does Marshall claim that Flies also exhibit such diversity in the natural world? In doing so, he sets himself apart from what more focused Dipterists have usually done, that is stay the course, look at one genus, one family, one region at a time; not much diversity displayed on their watch, for sure. Marshall, on the other hand, took on the entire dipteran Order, globally, with hundreds of superb photos (adults and immatures) that he took in the field over years of searching out fly species richness. He added an up-to-date text covering all taxa of the entire Order at the subfamily and tribal levels with hundreds of examples and images named at the genus and/or species level. Marshall’s FLIES is written and imaged for both the academic and the enthusiastic layperson. It contains some 2000 color images of flies in their natural environment, nearly all taken by the author. He has selected (out of some 160,000 named species of flies), those that he finds especially fascinating and those essential for demonstrating global dipteran diversity in form and function. The latter, form and function, speak directly to the astounding diversity and divergence of dipterans and justify including them along with the celebrated richness and abundance of beetles and parasitic wasps. Marshall, in FLIES, has justified that leap with his broad coverage and excellent story telling about flies’ lives. Such dipteran diversity is a mixed blessing for the public and for scientists alike. Who wants to see a blow fly from your neighbor’s garbage bin walking around on your chocolate cake, or a dozen mosquitos landing on you when you are trying patiently to photograph a hummingbird at a tropical flower in an Amazonian Heliconia patch? On the other hand, colorful hover flies on a yellow flower (Marshall’s book cover jacket) are part of nature’s exquisite beauty. While many groups of flies are vectors of devastating human and animal diseases and pests of crops and forests, others are important pollinators, recyclers, and beneficial control agents of insect pests. Marshall’s text tells compelling stories about all these kinds of flies that are easily read by citizen naturalists and high-schoolers in science class, as well as professional biologists of all walks of study. While Marshall’s introduction claims that “flies rule!” and his book sets out to prove that claim, Coleopterists and Hymenopterists certainly will argue that point. However, this reviewer has to agree that in the Amazon Basin during the rainy season there is more fly abundance by far in the rain forest canopy than any other group of insects (except relatively species-poor but abundant ants). This is thanks to one family of dipterans, the fungus gnats, Mycetophilidae (see Marshall’s pages 139-140) that emerge by the multimillions, as fungi reach their fruiting peak. While flies might not truly “rule,” gnats do have a lot to say about temperate and tropical fly abundance, something that Henri Fabre would appreciate. Without a doubt, Marshall sets high standards with this and his previous book, Insects: Their Natural History and Diversity, (also, Firefly Books). These treatments offer a challenge to entomologists of other ordinal taxa to produce similar books. Should that come to pass, and considering that insect and their relatives constitute three-fourths of life on earth, we humans would have a far better understanding of the natural world and our place in it.