Siouxsie Wiles

TL;DR: The literature of C. rodentium is reviewed from its emergence in the mid‐1960s to the most contemporary reports of colonization, pathogenesis, transmission and immunity, providing an excellent in vivo model for A/E lesion forming pathogens.

TL;DR: The functional expression of the whole bacterial lux operon in Mycobacterium tuberculosis and M. smegmatis is reported for the first time, allowing the development of auto-luminescent mycobacteria, and it is proved that both firefly and bacterial luciferases can be detected non-invasively in live mice by bioluminescence imaging.

TL;DR: It is demonstrated that primary colonization of the mouse by C. rodentium takes place within the caecum, specifically within the specialized patch of lymphoid tissue known as the caECal patch, and a bioluminescent C. rodentsium mutant failed to establish long‐term colonization, although low levels of bacteria could be recovered for up to 3 days post challenge from the Caecum.

TL;DR: The potential of this tetracycline-regulated system for the manipulation of mycobacterial gene expression inside and outside cells is demonstrated, indicating that intracellular bacteria are accessible and responsive to exogenously added tetrACYcline.

TL;DR: In this paper, the authors constructed optimized expression systems for several fluorescent proteins from the far-red region of the spectrum and analyzed their utility in several mycobacterial species, including tuberculosis, bovis and smegmatis.