Smita Srivastava

TL;DR: The present review highlights the nature, applications, perspectives and scale up of hairy root cultures for the production of valuable secondary metabolites.

TL;DR: Integration of transcriptomics, proteomics and metabolomics with system biology can aid in discovery of novel genes, transcriptional factors and several biosynthetic pathways which in turn can serve as a valuable tool for metabolic engineering and gene manipulation for enhancing the yield and productivity of secondary metabolites.

TL;DR: Various bioprocess optimization strategies that have been applied to sustain and enhance the product yield from the endophytes have been described in detail and techniques like mixed fermentation/co-cultivation and use of epigenetic modifiers have also been discussed as potential strategies to activate cryptic gene clusters in endophytic fungi, thereby aiding in novel metabolite discovery and overcoming the limitations associated with axenic culture.

TL;DR: The study indicates a dual role of ethanol, presumably as an elicitor and also as a carbon/energy source, leading to enhanced biomass and camptothecin production.

TL;DR: The cytotoxicity analysis of the crude camptothecin extract from the fungal biomass revealed its high effectiveness against colon and mammary gland cancer cell lines and provides immense scope for further enhancement in campthecin productivity at bioreactor level.