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Soheila Mohades

Bio: Soheila Mohades is an academic researcher from Old Dominion University. The author has contributed to research in topics: Cancer cell & Hydrogen peroxide. The author has an hindex of 8, co-authored 17 publications receiving 287 citations. Previous affiliations of Soheila Mohades include Shahid Beheshti University & University of Michigan.

Papers
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Journal ArticleDOI
TL;DR: Measurements of concentrations of hydrogen peroxide are reported on, a species known to have strong biological effects, produced by application of plasma to a minimum essential culture medium and results indicate that the plasma activated medium can kill the cancer cells in a dose dependent manner, retain its killing effect for several hours, and is as effective as apoptosis inducing drugs.
Abstract: The interaction of low temperature plasma with liquids is a relevant topic of study to the field of plasma medicine. This is because cells and tissues are normally surrounded or covered by biological fluids. Therefore, the chemistry induced by the plasma in the aqueous state becomes crucial and usually dictates the biological outcomes. This process became even more important after the discovery that plasma activated media can be useful in killing various cancer cell lines. Here, we report on the measurements of concentrations of hydrogen peroxide, a species known to have strong biological effects, produced by application of plasma to a minimum essential culture medium. The activated medium is then used to treat SCaBER cancer cells. Results indicate that the plasma activated medium can kill the cancer cells in a dose dependent manner, retain its killing effect for several hours, and is as effective as apoptosis inducing drugs.

122 citations

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TL;DR: Caspase-3 assays reveal that plasma can activate the cells' apoptotic pathways and alter cell morphology, cell reattachment, and kill SCaBER cells in an exposure time dependent and delayed manner.
Abstract: The efficacy of the plasma pencil is studied for its cancer therapeutics effects against the SCaBER cell line. First, the SCaBER cells in media were treated with different exposure times of low temperature plasma (LTP). Secondly, LTP activated media was generated by treating the media prior to adding it to the cells. Cell viability was assayed at 0, 24, 48, and 72 h after treatments. The results indicate that both treatments alter cell morphology, cell reattachment, and kill SCaBER cells in an exposure time dependent and delayed manner. Caspase-3 assays reveal that plasma can activate the cells' apoptotic pathways.

41 citations

Journal ArticleDOI
TL;DR: The effect of PAM on the viability of SCaBER cells, originally obtained from a bladder squamous cell carcinoma, is shown and its efficiency at different aging times is evaluated and there is a correlation between PAM efficiency and H2O2 concentration, as both decrease over time.
Abstract: Plasma-activated media (PAM) can be as effective as direct plasma treatment in killing cancer cells. PAM is produced by exposing liquid cell culture media to low temperature plasma. Reactive oxygen species (ROS) and reactive nitrogen species (RNS) in the aqueous state play key role in the anti-tumor effects of PAM. The effectiveness of PAM is highly dependent upon the dose of reactive species. The concentrations of reactive species and consequently the effectiveness of PAM decreases over time after plasma exposure. In this paper, the effect of PAM on the viability of SCaBER cells, originally obtained from a bladder squamous cell carcinoma, is shown and its efficiency at different aging times is evaluated. To investigate the selective effect of plasma on normal epithelial cells, MDCK (Madin-Darby Canine Kidney) cells from normal epithelial tissue of a dog kidney were treated by PAM. The concentrations of hydrogen peroxide at different times after plasma exposure were measured. Our findings indicate that there is a correlation between PAM efficiency and H2O2 concentration, as both decrease over time.

38 citations

Journal ArticleDOI
TL;DR: Results indicate that plasma consistently shows a delayed killing effect that is dose dependent, and there is some evidence that apoptosis is one of the pathways that leads to the death of the cells, indicating that plasma initiates cell signaling pathways.
Abstract: The application of low temperature plasmas in biology and medicine may lead to a paradigm shift in the way various diseases can be treated without serious side effects. Low temperature plasmas generated in gas mixtures that contain oxygen or air produce several chemically reactive species that have important biological implications when they interact with eukaryotic or prokaryotic cells. Here, a review of the effects of low temperature plasma generated by the plasma pencil on different cancerous cells is presented. Results indicate that plasma consistently shows a delayed killing effect that is dose dependent. In addition, there is some evidence that apoptosis is one of the pathways that leads to the death of the cells, indicating that plasma initiates cell signaling pathways.

38 citations

Journal ArticleDOI
TL;DR: In this paper, the authors report on the measurements of hydroxyl (OH) in the gas-liquid interface using laser-induced fluorescence and measurements of H2O2 concentration in biological media.
Abstract: Low-temperature, atmospheric pressure plasmas (LTPs) produce reactive oxygen and nitrogen species [O, O2−, O2 ( $^{1}\Delta $ ), hydroxyl (OH), H2O2, NO, NO2 $\ldots $ ]. In biological and medical applications, the concentrations and fluxes of these species play a crucial role in the biological outcomes. Many of these species are produced in the gaseous phase and at the gas-liquid interface when LTP is applied to biological media. In the medium, the plasma-produced oxygen reactive species and nitrogen reactive species generate long-lived species, such as hydrogen peroxide (H2O2), nitrites (NO2−), nitrates (NO3−), and organic peroxides (RO2). In particular, hydrogen peroxide is known to cause various oxidizing reactions in biological cells. One of the pathways to the creation of hydrogen peroxide is the reaction between OH radicals. Therefore, the measurement of OH concentration is of great importance. In this paper, we report on the measurements of OH in the gas-liquid interface using laser-induced fluorescence and measurements of H2O2 concentration in biological media. In addition, the effects of plasma activated media on cancer cells are briefly discussed.

37 citations


Cited by
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TL;DR: In this paper, the most important mechanisms of generation and transport of the key species in the plasmas of atmospheric-pressure plasma jets and other non-equilibrium atmospheric pressure plasms are introduced and examined from the viewpoint of their applications in plasma hygiene and medicine and other relevant fields.

825 citations

Journal ArticleDOI
TL;DR: This review provides a comprehensive introduction of the basics of CAP, state of the art research in this field, the primary challenges, and future directions to cancer biologists.
Abstract: // Dayun Yan 1 , Jonathan H. Sherman 2 and Michael Keidar 1 1 Department of Mechanical and Aerospace Engineering, The George Washington University, NW, Washington, DC, USA 2 Neurological Surgery, The George Washington University, NW, Washington, DC, USA Correspondence to: Dayun Yan, email: // Michael Keidar, email: // Keywords : cold plasma, cancer treatment, reactive species, selectivity Received : September 14, 2016 Accepted : October 29, 2016 Published : November 11, 2016 Abstract Over the past decade, cold atmospheric plasma (CAP), a near room temperature ionized gas has shown its promising application in cancer therapy. Two CAP devices, namely dielectric barrier discharge and plasma jet, show significantly anti-cancer capacity over dozens of cancer cell lines in vitro and several subcutaneous xenograft tumors in vivo. In contrast to conventional anti-cancer approaches and drugs, CAP is a selective anti-cancer treatment modality. Thus far establishing the chemical and molecular mechanism of the anti-cancer capacity of CAP is far from complete. In this review, we provide a comprehensive introduction of the basics of CAP, state of the art research in this field, the primary challenges, and future directions to cancer biologists.

375 citations

Journal ArticleDOI
TL;DR: This review presents a succinct review of how novel, efficient methods based on non-equilibrium reactive plasma chemistries can be applied to low-cost natural water sources to produce a prospective product with a wide range of applications while at the same time minimising the process steps and dramatically reducing the use of expensive and/or hazardous reagents.
Abstract: Novel plasma-based technologies that offer maximum efficiency at minimal environmental costs are expected to further promote the sustainable societal and economic development. Unique transfer of chemical reactivity and energy from gaseous plasmas to water takes place in the absence of any other chemicals, but results in a product with a notable transient broad-spectrum biological activity, referred to as plasma-activated water (PAW). These features make PAW a green prospective solution for a wide range of biotechnology applications, from water purification to biomedicine. Here, we present a succinct review of how novel, efficient methods based on non-equilibrium reactive plasma chemistries can be applied to low-cost natural water sources to produce a prospective product with a wide range of applications while at the same time minimising the process steps and dramatically reducing the use of expensive and/or hazardous reagents. Despite the recent exciting developments in this field, there presently is no topical review which specifically focuses on the underlying physics and chemistry related to plasma-activated water. We focus specifically on the PAW generation, origin of reactive species present in PAW, its related analytical chemistry and potentially different mechanisms that regulate the bio-activities of PAW in different biotech-applications and their roles in determining PAW efficacy and selectivity. We then review recent advances in our understanding of plasma-water interactions, briefly outlining current and proposed applications of PAW in agriculture, food and biomedicine. Finally, we outline future research directions and challenges that may hinder translation of these technologies into real-life applications. Overall, this review will provide much needed insights into the fundamental aspects of PAW chemistry required for optimization of the biochemical activity of PAW and translation of this environment- and human-health-friendly, and energy-efficient strategy into real life applications.

242 citations