S
Sonya Clark
Researcher at Pacific Biosciences
Publications - 16
Citations - 4019
Sonya Clark is an academic researcher from Pacific Biosciences. The author has contributed to research in topics: Polymerase & Nucleic acid. The author has an hindex of 10, co-authored 16 publications receiving 3836 citations. Previous affiliations of Sonya Clark include United States Department of Agriculture & University of California, Davis.
Papers
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Journal ArticleDOI
Real-Time DNA Sequencing from Single Polymerase Molecules
John Eid,Adrian Fehr,Jeremy Gray,Khai Luong,John Lyle,Geoff Otto,Paul Peluso,David R. Rank,Primo Baybayan,Brad Bettman,Arkadiusz Bibillo,Keith Bjornson,Bidhan Chaudhuri,Fred Christians,Ronald L. Cicero,Sonya Clark,Ravindra V. Dalal,Alex DeWinter,John Dixon,Mathieu Foquet,Alfred Gaertner,Paul Hardenbol,Cheryl Heiner,Kevin Hester,David P. Holden,Gregory J. Kearns,Xiangxu Kong,Ronald Kuse,Yves Lacroix,Steven Lin,Paul Lundquist,Congcong Ma,Patrick Marks,Mark Maxham,Devon Murphy,Insil Park,Thang Pham,Michael Phillips,Joy Roy,Robert Sebra,Gene Shen,Jon M. Sorenson,Austin B. Tomaney,Kevin Travers,Mark Trulson,John Vieceli,Jeffrey Wegener,Dawn Wu,Alicia Yang,Denis Zaccarin,Peter Zhao,Frank Zhong,Jonas Korlach,Stephen Turner +53 more
TL;DR: Single-molecule, real-time sequencing data obtained from a DNA polymerase performing uninterrupted template-directed synthesis using four distinguishable fluorescently labeled deoxyribonucleoside triphosphates (dNTPs) are presented.
Journal ArticleDOI
A folded protein can be transported across the chloroplast envelope and thylakoid membranes.
Sonya Clark,Steven M. Theg +1 more
TL;DR: Import of prOE17-BPTI into isolated chloroplasts and thylakoids demonstrates that the small tightly folded BPTI domain is carried across both the chloroplast envelopes and the delta pH-dependent transmembrane transporter of the thylAKoid membrane when linked to the correctly targeted OE17 precursor.
Patent
Polymerases for nucleotide analogue incorporation
David K. Hanzel,Geoff Otto,Devon Murphy,Paul Peluso,Thang Pham,David R. Rank,Paul Mitsis,Fred Christians,Arek Bibillo,Insil Park,Sonya Clark,John Lyle +11 more
TL;DR: In this article, compositions of polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogue in the active site region are provided.
Journal ArticleDOI
Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides.
Jonas Korlach,Arek Bibillo,Jeffrey Wegener,Paul Peluso,Thang Pham,Insil Park,Sonya Clark,Geoff Otto,Stephen Turner +8 more
TL;DR: Results presented here show the compatibility of these nucleotides for single-molecule, real-time DNA sequencing applications.
Patent
Generation of modified polymerases for improved accuracy in single molecule sequencing
Sonya Clark,Arek Bibillo,Paul Peluso,Fred Christians,Molly He,Insil Park,Harold Lee,Keith Bjornson,Lei Jia,Robin Emig +9 more
TL;DR: In this paper, the branching fractions of modified recombinant polymerases are compared to the branching fraction of the parent polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog.