Author
Spiros A. Pergantis
Other affiliations: University of Manchester, United States Environmental Protection Agency, Utrecht University ...read more
Bio: Spiros A. Pergantis is an academic researcher from University of Crete. The author has contributed to research in topics: Mass spectrometry & Inductively coupled plasma mass spectrometry. The author has an hindex of 32, co-authored 103 publications receiving 2648 citations. Previous affiliations of Spiros A. Pergantis include University of Manchester & United States Environmental Protection Agency.
Papers published on a yearly basis
Papers
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TL;DR: Trace element concentrations in sediment were investigated at four fish farms in the Eastern Mediterranean Sea and found that, in anoxic sediments, all elements had higher concentrations at the impacted stations than at reference stations while in oxic sediment, many elemental concentrations were lower at impacts than atreference stations.
113 citations
TL;DR: A sensitive protocol for unambiguously and positively identifying O-glycosylation sites in glycopeptides is described, based on beta-elimination of the glycan chain using NH4OH, and it is demonstrated that site determination can be achieved using as little as 1 pmol of starting material.
101 citations
TL;DR: This study reports on the development of a hyphenated analytical technique for the detection and characterization of metal-containing NPs, i.e., their metal mass fraction, size, and number concentration.
Abstract: Nanoparticle (NP) determination has recently gained considerable interest since a growing number of engineered NPs are being used in commercial products. As a result, their potential to enter the environment and biological systems is increasing. In this study, we report on the development of a hyphenated analytical technique for the detection and characterization of metal-containing NPs, i.e., their metal mass fraction, size, and number concentration. Hydrodynamic chromatography (HDC), suitable for sizing NPs within the range of 5 to 300 nm, was coupled online to inductively coupled plasma mass spectrometry (ICPMS), providing for an extremely selective and sensitive analytical tool for the detection of NPs. However, a serious drawback when operating the ICPMS in its conventional mode is that it does not provide data regarding NP number concentrations and, thus, any information about the metal mass fraction of individual NPs. To address this limitation, we developed single particle (SP) ICPMS coupled onlin...
92 citations
TL;DR: In this article, an octadecyldimethylsilyl reversed-phase narrow-bore HPLC column was used for the high-speed separation of several biologically and environmentally important arsenic compounds.
Abstract: The growing importance of arsenic speciation analysis has led to the development of a wide range of high-performance liquid chromatographic based hyphenated techniques. During the present study a method was developed for the high-speed separation of several biologically and environmentally important arsenic compounds. The method is based on the use of an octadecyldimethylsilyl reversed-phase narrow-bore HPLC column. Separation of anionic arsenic species [arsenite (Aite), dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), and arsenate (Aate)] can be achieved using a mobile phase containing 5 mM tetrabutylammonium hydroxide as the ion-pairing reagent, at pH 6.0, in less than 2 min, when employing a flow rate of 0.7 ml min−1. Adding 4-hydroxyphenylarsonic acid as the internal standard prolongs the total separation time by 30 s. On-line coupling with inductively coupled plasma mass spectrometry affords high sensitivity, as well as low limits of detection (low ppb or pg of arsenic). The influence of mobile phase pH and ion-pairing reagent concentration on the separation efficiency was studied. A loss of resolution occurs with increasing ion-pairing reagent concentration; the optimum pH is between 6.0 and 6.2. The ion-pair reversed-phase narrow-bore HPLC-ICP-MS method was subsequently applied to the speciation of arsenic in wine and kelp samples. Aite at trace levels was found to be the only arsenic species present in several wines. Average spike recoveries for Aite, Aate, MMAA and DMAA were 95 ± 3, 94 ± 5, 98 ± 1 and 92 ± 1%, respectively, for all wines examined. The method was also used for the speciation of four arsenosugars and DMAA in a kelp powder extract.
87 citations
TL;DR: An analytical method based on reversed-phase microbore HPLC coupled with electrospray mass spectrometry (ES-MS) is described in this paper, which allows for the determination of up to ten organoarsenicals in a single chromatographic run.
Abstract: An analytical method based on reversed-phase microbore HPLC coupled
on-line with electrospray mass spectrometry (ES-MS) is described. The
method allows for the determination of up to ten organoarsenicals in a
single chromatographic run. Excellent sensitivity and selectivity is
achieved by operating the triple quadrupole mass spectrometer in the
selected-reaction monitoring mode. This is possible because the ions
produced by collision-induced dissociation of the protonated molecules or
molecular ions of the ten organoarsenicals are characteristic of each
compound. The best LODs, achieved in the positive-ion mode, were between 2
and 21 pg of arsenic and corresponded to arsenicals which exist as cations
in acidic solutions. The selectivity achieved by using this method allows
for successful determination of arsenicals co-eluting during HPLC. This is
a major improvement over other hyphenated techniques already used for
arsenic speciation,
e.g.
, HPLC–ICP-MS. Furthermore, the
method was used for the analysis of an undiluted urine SRM in which
arsenobetaine was determined to be present at the low µg
l
-1
level.
86 citations
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TL;DR: This review covers the literature published in 2014 for marine natural products, with 1116 citations referring to compounds isolated from marine microorganisms and phytoplankton, green, brown and red algae, sponges, cnidarians, bryozoans, molluscs, tunicates, echinoderms, mangroves and other intertidal plants and microorganisms.
4,649 citations
Book•
01 Jan 2013
TL;DR: In this article, the authors defined the sources of heavy metals and metalloids in Soils and derived methods for the determination of Heavy Metals and Metalloids in soil.
Abstract: Preface.- Contributors.- List of Abbreviations.- Section 1: Basic Principles: Introduction.-Sources of Heavy Metals and Metalloids in Soils.- Chemistry of Heavy Metals and Metalloids in Soils.- Methods for the Determination of Heavy Metals and Metalloids in Soils.- Effects of Heavy Metals and Metalloids on Soil Organisms.- Soil-Plant Relationships of Heavy Metals and Metalloids.- Heavy Metals and Metalloids as Micronutrients for Plants and Animals.-Critical Loads of Heavy Metals for Soils.- Section 2: Key Heavy Metals And Metalloids: Arsenic.- Cadmium.- Chromium and Nickel.- Cobalt and Manganese.- Copper.-Lead.- Mercury.- Selenium.- Zinc.- Section 3: Other Heavy Metals And Metalloids Of Potential Environmental Significance: Antimony.- Barium.- Gold.- Molybdenum.- Silver.- Thallium.- Tin.- Tungsten.- Uranium.- Vanadium.- Glossary of Specialized Terms.- Index.
1,684 citations
TL;DR: Antimony is ubiquitously present in the environment as a result of natural processes and human activities as discussed by the authors and is considered to be priority pollutants interest by the USEPA and the EU.
1,051 citations
TL;DR: This work highlights this rapidly advancing area of algal science with a particular focus on the key research required to assess better the health benefits of an alga or algal product.
Abstract: Global demand for macroalgal and microalgal foods is growing, and algae are increasingly being consumed for functional benefits beyond the traditional considerations of nutrition and health. There is substantial evidence for the health benefits of algal-derived food products, but there remain considerable challenges in quantifying these benefits, as well as possible adverse effects. First, there is a limited understanding of nutritional composition across algal species, geographical regions, and seasons, all of which can substantially affect their dietary value. The second issue is quantifying which fractions of algal foods are bioavailable to humans, and which factors influence how food constituents are released, ranging from food preparation through genetic differentiation in the gut microbiome. Third is understanding how algal nutritional and functional constituents interact in human metabolism. Superimposed considerations are the effects of harvesting, storage, and food processing techniques that can dramatically influence the potential nutritive value of algal-derived foods. We highlight this rapidly advancing area of algal science with a particular focus on the key research required to assess better the health benefits of an alga or algal product. There are rich opportunities for phycologists in this emerging field, requiring exciting new experimental and collaborative approaches.
933 citations