S
Stefan J. Haugen
Researcher at National Renewable Energy Laboratory
Publications - 15
Citations - 254
Stefan J. Haugen is an academic researcher from National Renewable Energy Laboratory. The author has contributed to research in topics: Chemistry & Pseudomonas putida. The author has an hindex of 2, co-authored 8 publications receiving 41 citations.
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Journal ArticleDOI
Outer membrane vesicles catabolize lignin-derived aromatic compounds in Pseudomonas putida KT2440
Davinia Salvachúa,Davinia Salvachúa,Allison Z. Werner,Allison Z. Werner,Isabel Pardo,Martyna Michalska,Brenna A. Black,Bryon S. Donohoe,Stefan J. Haugen,Rui Katahira,Sandra Notonier,Sandra Notonier,Kelsey J. Ramirez,Antonella Amore,Samuel O. Purvine,Erika M. Zink,Paul E. Abraham,Richard J. Giannone,Suresh Poudel,Philip D. Laible,Robert L. Hettich,Gregg T. Beckham,Gregg T. Beckham +22 more
TL;DR: This work proposes a mechanism for extracellular nutrient acquisition from aromatic compounds by soil bacteria, which holds promise for improving the efficiency of microbial lignin conversion and suggests that OMVs could potentially be useful tools for synthetic biology and biotechnological applications.
Journal ArticleDOI
Mixed plastics waste valorization through tandem chemical oxidation and biological funneling
Kevin P. Sullivan,Allison Z. Werner,Kelsey J. Ramirez,Lucas D. Ellis,Jeremy R. Bussard,Brenna A. Black,David G. Brandner,Felicia Bratti,Bonnie L. Buss,Xueming Dong,Stefan J. Haugen,Morgan A Ingraham,Mikhail O. Konev,William E. Michener,Joel Miscall,Isabel Pardo,Sean P. Woodworth,Adam M. Guss,Yuriy Román-Leshkov,Shannon S. Stahl,Gregg T. Beckham +20 more
TL;DR: This work shows that metal-catalyzed autoxidation depolymerizes comingled polymers into a mixture of oxygenated small molecules that are advantaged substrates for biological conversion, and engineer a robust soil bacterium to funnel these oxygenated compounds into a single exemplary chemical product.
Journal ArticleDOI
Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440.
Allison Z. Werner,Rita Clare,Mand Thomas David,Isabel Pardo,Kelsey J. Ramirez,Stefan J. Haugen,Felicia Bratti,Gara N. Dexter,Joshua R. Elmore,Jay D. Huenemann,George L. Peabody,Christopher W. Johnson,Nicholas A. Rorrer,Davinia Salvachúa,Adam M. Guss,Gregg T. Beckham +15 more
TL;DR: In this paper, the authors performed four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2IIA3IIBIIA1II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) TPA hydrolysis to TPA
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Pathway discovery and engineering for cleavage of a β-1 lignin-derived biaryl compound.
Gerald N. Presley,Allison Z. Werner,Allison Z. Werner,Rui Katahira,David C. Garcia,David C. Garcia,Stefan J. Haugen,Kelsey J. Ramirez,Richard J. Giannone,Gregg T. Beckham,Gregg T. Beckham,Joshua K. Michener +11 more
TL;DR: A pathway for cleavage of 1,2-diguaiacylpropane-1,3-diol (DGPD), a β-1 linked biaryl representative of a ring-opened spirodienone linkage, in Novosphingobium aromaticivorans DSM12444 was identified in this paper.
Journal ArticleDOI
Particle Size Reduction of Poly(ethylene terephthalate) Increases the Rate of Enzymatic Depolymerization But Does Not Increase the Overall Conversion Extent
Richard K. Brizendine,Erika Erickson,Stefan J. Haugen,Kelsey J. Ramirez,Joel Miscall,Davinia Salvachúa,Andrew R. Pickford,Margaret J. Sobkowicz,John McGeehan,Gregg T. Beckham +9 more
TL;DR: In this article , the effect of particle size and crystallinity on the performance of a variant of the leaf compost-cutinase enzyme (LCC-ICCG) was investigated.