S
Steven F. Lee
Researcher at University of Cambridge
Publications - 88
Citations - 4153
Steven F. Lee is an academic researcher from University of Cambridge. The author has contributed to research in topics: Microscopy & Fluorescence-lifetime imaging microscopy. The author has an hindex of 30, co-authored 79 publications receiving 3113 citations. Previous affiliations of Steven F. Lee include Stanford University & University of Sussex.
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Journal ArticleDOI
3D structures of individual mammalian genomes studied by single-cell Hi-C
Tim J. Stevens,Tim J. Stevens,David Lando,Srinjan Basu,Liam P Atkinson,Yang Cao,Steven F. Lee,Martin Leeb,Kai J. Wohlfahrt,Wayne Boucher,Aoife O’Shaughnessy-Kirwan,Julie Cramard,Andre J. Faure,Meryem Ralser,Enrique Blanco,Lluis Morey,Miriam Sansó,Matthieu Palayret,Ben Lehner,Ben Lehner,Ben Lehner,Luciano Di Croce,Luciano Di Croce,Luciano Di Croce,Anton Wutz,Brian Hendrich,Dave Klenerman,Ernest D. Laue +27 more
TL;DR: 3D structures of entire mammalian genomes are calculated using data from a new chromosome conformation capture procedure that allows genome folding to be examined at a scale of less than 100 kb, and chromosome structures to be validated.
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A spindle-like apparatus guides bacterial chromosome segregation
Jerod L. Ptacin,Steven F. Lee,Ethan C. Garner,Esteban Toro,Michael Eckart,Luis R. Comolli,W. E. Moerner,Lucy Shapiro +7 more
TL;DR: A bacterial chromosome segregation mechanism that features basic operating principles similar to eukaryotic mitotic machines is elucidated, including a multivalent protein complex at the centromere that stimulates the dynamic disassembly of polymers to move chromosomes into daughter compartments.
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Initiation of T cell signaling by CD45 segregation at 'close contacts'
Veronica T. Chang,Ricardo A. Fernandes,Kristina A. Ganzinger,Steven F. Lee,Christian Siebold,James McColl,Peter Jönsson,Matthieu Palayret,Karl Harlos,C.H. Coles,E. Yvonne Jones,Yuan Lui,Elizabeth Huang,Robert J.C. Gilbert,David Klenerman,A. Radu Aricescu,Simon J. Davis +16 more
TL;DR: The structural basis for, and the potent signaling effects of, local CD45 and kinase segregation are revealed, and TCR ligands have the potential to heighten signaling simply by holding receptors in close contacts.
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Single-molecule visualization of DNA G-quadruplex formation in live cells
Marco Di Antonio,Marco Di Antonio,Aleks Ponjavic,Aleks Ponjavic,Antanas Radzevičius,Rohan T. Ranasinghe,Marco Catalano,Xiaoyun Zhang,Jiazhen Shen,Lisa-Maria Needham,Steven F. Lee,David Klenerman,Shankar Balasubramanian +12 more
TL;DR: A G4-specific fluorescent probe (SiR-PyPDS) has been developed that enables single-molecule and real-time detection of individual G4 structures in living cells without perturbing G4 formation and dynamics.
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Brightening, Blinking, Bluing and Bleaching in the Life of a Quantum Dot: Friend or Foe?
Steven F. Lee,Mark A. Osborne +1 more
TL;DR: This work reviews recent experimental and theoretical studies of FI, PLE and bluing, highlighting the benefits, as well as complications, they bring to key applications.