S
Steven P. Gygi
Researcher at Harvard University
Publications - 778
Citations - 147003
Steven P. Gygi is an academic researcher from Harvard University. The author has contributed to research in topics: Proteome & Phosphorylation. The author has an hindex of 172, co-authored 704 publications receiving 129173 citations. Previous affiliations of Steven P. Gygi include University of Rochester Medical Center & Cell Signaling Technology.
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Book ChapterDOI
The absolute quantification strategy: application to phosphorylation profiling of human separase serine 1126.
TL;DR: This chapter discusses the development of an AQUA method and demonstrates its usefulness in the measurement of endogenous levels of the human protein separase at a functionally relevant phosphorylation site, serine 1126.
Journal ArticleDOI
Alternative Ubiquitin Activation/Conjugation Cascades Interact with N-end rule Ubiquitin Ligases to Control Degradation of RGS Proteins
TL;DR: It is demonstrated that UBA6-USE1 functions with the UBR1-3 subfamily of N-recognin E3s to degrade the N-end rule substrates RGS4, RGS5, and Arg (R)-GFP, and suggests that the UBA 6 and UBA1 pathways can function in parallel with the same E3 to degradeThe same targets in a spatially distinct manner.
Journal ArticleDOI
Combining genetic perturbations and proteomics to examine kinase-phosphatase networks in Drosophila embryos.
Richelle Sopko,Marianna Foos,Arunachalam Vinayagam,Bo Zhai,Richard Binari,Richard Binari,Yanhui Hu,Sakara Randklev,Sakara Randklev,Lizabeth A. Perkins,Steven P. Gygi,Norbert Perrimon,Norbert Perrimon +12 more
TL;DR: A validated set of transgenic RNA-interference reagents for knockdown and characterization of all protein kinases and phosphatases present during early Drosophila melanogaster development and shows how correlative phosphorylation at the site level can indicate function.
Journal ArticleDOI
Mass Spectrometric Analysis of the Editosome and Other Multiprotein Complexes in Trypanosoma brucei
TL;DR: The composition of the editosome, a multi-protein complex that catalyzes uridine insertion and deletion RNA editing to produce mature mitochondrial mRNAs in trypanosomes, was analyzed by mass spectrometry and contributed to the functional annotation of T. brucei genome.
Journal ArticleDOI
BioPlex Display: An Interactive Suite for Large-Scale AP-MS Protein-Protein Interaction Data.
TL;DR: BioPlex Display is developed, which integrates individual protein querying, access to empirical data, and on-the-fly annotation of networks within an easy-to-use and mobile web application that enables exploration and dissemination of the growing BioPlex interaction network.