Author
Subash C. B. Gopinath
Other affiliations: University of Malaya, Annamalai University, Anna University ...read more
Bio: Subash C. B. Gopinath is an academic researcher from Universiti Malaysia Perlis. The author has contributed to research in topics: Aptamer & Medicine. The author has an hindex of 45, co-authored 455 publications receiving 7855 citations. Previous affiliations of Subash C. B. Gopinath include University of Malaya & Annamalai University.
Topics: Aptamer, Medicine, Biosensor, Materials science, Detection limit
Papers published on a yearly basis
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TL;DR: This review provides an overview of the strategies involved in aptamer-based ELASA, a systematic evolution of Ligands by Exponential Enrichment (SELEX) method that can generate a nucleic acid-based probe (aptamer) that possess numerous advantages compared to antibodies.
434 citations
TL;DR: The separation methods used in SELEX processes, which allow various aptamers to be developed against a wide variety of molecules, irrespective of the target size, are reviewed.
Abstract: SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification. With the development of aptamer technology over the last decade, various modified SELEX processes have arisen that allow various aptamers to be developed against a wide variety of molecules, irrespective of the target size. In the present review, the separation methods used in such SELEX processes are reviewed.
363 citations
TL;DR: The formation of purple-colored aggregates from the red dispersed (repulsion) state of GNPs in the presence of mono- or divalent ions is the key principle behind this assay, and can be an alternative to existing diagnostic assays.
206 citations
TL;DR: In vitro selections were carried out to find aptamers that specifically bind and distinguish the closely related human influenza A virus subtype H3N2, and the selected aptamer, P30-10-16, binds specifically to the haemagglutinin region of the target strain A/Panama/2007/1999(H3N 2) and failed to recognize other human influenza viruses.
Abstract: Aptamers selected against various kinds of targets have shown remarkable specificity and affinity, similar to those displayed by antibodies to their antigens. To employ aptamers as genotyping reagents for the identification of pathogens and their strains, in vitro selections were carried out to find aptamers that specifically bind and distinguish the closely related human influenza A virus subtype H3N2. The selected aptamer, P30-10-16, binds specifically to the haemagglutinin (HA) region of the target strain A/Panama/2007/1999(H3N2) and failed to recognize other human influenza viruses, including another strain with the same subtype, H3N2. The aptamer displayed over 15-fold-higher affinity to the HA compared with the monoclonal antibody, and efficiently inhibited HA-mediated membrane fusion. These studies delineate the application of aptamers in the genotyping of viruses.
187 citations
TL;DR: Aptamers are single stranded DNA or RNA oligonucleotides that have high affinity and specificity towards a wide range of target molecules that have become the elemental tool for numerous biosensor developments by amalgamating with a number of methods.
171 citations
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Journal Article•
28,685 citations
28 Jul 2005
TL;DR: PfPMP1）与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1（PfPMP1）与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用，在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员，通过启动转录不同的var基因变异体为抗原变异提供了分子基础。
18,940 citations
TL;DR: These factors have been shown to substantially affect the biodistribution and blood circulation half-life of circulating nanoparticles by reducing the level of nonspecific uptake, delaying opsonization, and increasing the extent of tissue specific accumulation.
Abstract: Nanoparticle (NP) drug delivery systems (5−250 nm) have the potential to improve current disease therapies because of their ability to overcome multiple biological barriers and releasing a therapeutic load in the optimal dosage range. Rapid clearance of circulating nanoparticles during systemic delivery is a critical issue for these systems and has made it necessary to understand the factors affecting particle biodistribution and blood circulation half-life. In this review, we discuss the factors which can influence nanoparticle blood residence time and organ specific accumulation. These factors include interactions with biological barriers and tunable nanoparticle parameters, such as composition, size, core properties, surface modifications (pegylation and surface charge), and finally, targeting ligand functionalization. All these factors have been shown to substantially affect the biodistribution and blood circulation half-life of circulating nanoparticles by reducing the level of nonspecific uptake, de...
3,009 citations