Suhad M. Hamdoon

Bio: Suhad M. Hamdoon is an academic researcher from University of Mosul. The author has contributed to research in topics: Enterococcus faecium & Chemistry. The author has an hindex of 1, co-authored 3 publications receiving 3 citations.

Prevalence of Anaerobic Bacteria in Periodontitis in Relation to Pocket Depth

Abstract: Aims: : The study evaluated the prevalence of anaerobic bacteria in the subgingival plaque in periodontitis in relation to pocket depth. Materials and Methods :The study was performed on 97 sub-gingival plague sample , pockets depth were measured , anaerobic bacteria were isolated and identified in relation to pockets depth. Results: In this study high prevalence of anaerobic bacteria in the periodontal pockets of patients suffering from periodontitis compared with the gingival sulcus of healthy subjects with marked shifting from mainly Gram positive facultative anaerobic bacteria in shallow pockets to mainly Gram-negative strict anaerobic bacteria in the deep pockets.Conclusion:. Anaerobic culture used in this study provided information about the susceptibility of the individuals to develop periodontal diseases. on bone.

Molecular detection and identification of Enterococcus faecium isolated from dental root canals

Abstract: Enterococci are usually encountered and predominate in oral infections, especially those associated with dental root canal infections of necrotic pulp and periodontitis. This study aimed to detect and identify Enterococcus faecium isolated from infected root canals, using polymerase chain reaction ( PCR). Thirty samples were collected from patients with necrotic pulp, infected root canals, and endodontic treatment failure, attending the Conservative Treatment Department, College of Dentistry, Mosul University, Dental Teaching Hospital. The samples were obtained by inserting sterile paper points into the root canals and transferred in brain heart infusion broth vials to be inoculated in a selective M-Enterococcus Agar Base . Twenty five isolates that belong to the genus Enterococcus were recognized by traditional culture methods and biochemical tests. Then, DNA extractions of these isolates were carried out for identification with PCR by the amplification of ddI (D-Ala-D-Ala Ligase) chromosomal genes of Enterococcus faecium. Among the 25 isolates, twenty (80%) were identified to the level of Enterococcus faecium by traditional culture methods and biochemical tests, in comparison to 17 (68%) identified by molecular identification. The PCR products for the specific primer produced bands on agarose gel at the position of 658bp. The study showed that the use of PCR with primers for the E. faecium ddI gene may be the most accurate method for rapid identification of Enterococci. Molecular identification of Enterococcus spp. revealed a significant role of E. feacium in root canal infections. Also, the detection of ddI gene using PCR provides a definitive target that could be used for the detection of E. faecium from clinical samples.

Evaluation of biofilm formation on different clear orthodontic retainer materials

Abstract: Aim: To assess the chemical composition and oral biofilm formation on different types of commercially available clear orthodontic retainer materials (CORM). Materials and Methods: Four types of CORM commercially available were used (Clear advantage series I (CAS1), Clear advantage series II (CAS2), Endure (ES), and CENTRI FORM-clear rigid material (CFCRM)). Circular samples (12 mm diameter) of each CORM were prepared for (n = 40). Unstimulated saliva from twenty volunteers was collected. Fourier Transformation Infrared Spectroscopy (FTIR) was used for the evaluation of the chemical composition of CORM. For the quantitative assessment of oral biofilm formation, samples of each CORM were incubated for twenty-four hours, and crystal violet assay (CVA) was utilized. The degree of absorbance was measured using a spectrophotometer at 570 nm. For qualitative evaluation of oral formation, the samples of each CORM were incubated for 24 hours, and viable biofilm cells stained by acridine orange were examined under a fluorescent microscope. Results: FTIR findings showed that CAS2 was made of polypropylene and ES is made of polyvinyl chloride, while others were made of co-polyester. CVA results confirmed that CAS2 showed the lowest biofilm formation, which differs significantly compared to CAS1, CFCRM, and ES. No significant difference in biofilm formation was detected between CAS1, CFCRM, and ES. Viable biofilm cells staining by acridine orange showed that CAS2 demonstrated smaller microcolonies of viable biofilm cells compared with CAS1, CFCRM, and ES, which confirmed the result obtained by CVA. Conclusions: CAS2 showed anti-microbial activities with a decrease the in vitro biofilm formation, which may be related to its chemical composition.

Oral probiotic and its delivery carriers to improve oral health: A review.

Abstract: In recent years, oral probiotics have been researched on their effectiveness in reducing and preventing oral diseases. Oral probiotics could be introduced into the oral cavity to keep the equilibrium of the microbiome. Hence, the delivery carrier for oral probiotics plays an important factor to ensure a high number of oral probiotics were delivered and released into the oral cavity. This review presents a brief overview of oral microbiota and the role of oral probiotics in reducing oral diseases. Moreover, important aspects of the oral probiotic product such as viability, adherence ability, health effects, safety, and delivery site were discussed. Besides that, the importance of utilizing indigenous oral probiotics was also emphasized. Oral probiotics are commonly found in the market in the form of chewing tablets, lozenges, and capsules. Hence, the oral probiotic carriers currently used in the market and research were reviewed. Furthermore, this review introduces new potential oral probiotic delivery carriers such as oral strip, bucco-adhesive gel, and mouthwash. Their effectiveness in delivering oral probiotics for oral health was also explored.

Antibacterial Effect of Black and Green Tea on Oral Bacteria in Pregnant Women

Abstract: Aims: To evaluate the antibacterial effect of black and green tea on aerobic and anaerobic bacteria in pregnant women. Materials and Methods: Sixty pregnant women were submitted for this study . The samples were collected by two different methods, then the alcohol and boiling water extract of black and green tea were used to study the antibacterial effect on eight types of bacteria :Staphylococcus species ,Lactobacillus species ,Oral Streptococci, Porphyromonas species, Prevotella species, Actinobacilus actinomycetecomitans, Fusobacterium species and Actinomyces species. The results: There is a significant difference between the number of bacterial isolates in pregnant and non-pregnant women in Staphylococcus spp. s, Prevotella spp, Actinobacilus actinomycetecomitans , Fusobacterium spp. and Actinomyces spp. While in two methods used to collect the samples there was a significant difference in Prevotella spp. And the extracts showed antibacterial activity against bacteria at different concentration .Thus the best extract that can affect to the bacteria was Boiling water extract of Green tea. Conclusion: The boiling water extract of green tea was effective to oral bacteria ,especially periodontal pathogens, so we suggest to use it as mouthwash for the treatment of periodontitis.

The Effect of Different Obturation Techniques in Primary Teeth on the Apical Microleakage using Endoflas: A Comparative In Vitro Study

Abstract: Objectives This study was carried out to quantitatively evaluate and compare the sealing ability of Endoflas by using different obturation techniques. Materials and Methods After 42 extracted primary maxillary incisors and canines were decoronated, their canals were instrumented with K files of size ranging from #15 to #50. In accordance with the obturation technique, the samples were divided into three experimental groups, namely, group I: endodontic pressure syringe, group II: modified disposable syringe, and group III: reamer technique, and two control groups. Dye extraction method was used for leakage evaluation. Data were analyzed using one-way ANOVA and Dunnett's T3 post hoc tests. The level of significance was set at p < 0.05. Results Endodontic pressure syringe had significantly less leakage than the modified disposable syringe and reamer techniques (p < 0.05). Meanwhile, no significant difference was found in the mean leakage between the modified disposable syringe and the reamer techniques. Conclusion Amongst all the techniques used in this study, endodontic pressure syringe could be preferred as an obturation technique in primary teeth when used with Endoflas obturation material because of its potential to provide good apical seal.

Effect of Plasma Liquid on Colloidal (CoNPs) and (PtNPs) Nanostructures Produced by Laser Ablation Technique for Antibacterial Applications

Abstract: Abstract In this work, the biological and physical properties of colloidal platinum (PtNPs) and cobalt (CoNPs) nanoparticles were examined for antibacterial applications. The colladial PtNPs and CoNPs nanoparticles were produced using two different liquids (double-distilled water (DDDW) and Dulbecco’s Modified Eagle’s Medium (DMEM)) by laser ablation technique (PLAL). Characterization techniques such as transmission electron microscopy (TEM), UV-vis spectroscopy and Fourier transform infrared (FTIR) were used to determine some physical properties of PtNPs and CoNPs. The antibacterial effects ofcolladial PtNPs and CoNPs at different doses on various bacteria were investigated and their antibacterial activity resistances were calculated by measuring the inhibition zone widths. The effect of the liquid used in the production, the type of bacteria and dose amounts on the antibacterial resistance of these nanoparticles were determined. In this study, it has been shown that the liquid used in the production of PtNPs and CoNPs can create significant changes in the physical and antibacterial properties of nanoparticles, especially by changing the size and concentration of collodial PtNPs.