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Showing papers by "Susan Lindquist published in 1986"


Journal ArticleDOI
TL;DR: A comparison of different Organisms and Stages of Development and Heat-Induced Lethality and Thermotolerance and the role of RNA Processing are presented.
Abstract: PERSPECTIVES AND SUMMARY . . . . . 1151 CHARACTERIZATION OF THE RESPONSE 1153 Comparison: Different Organisms and Stages of Development. ll53 The Proteins Induced by Heat ... . 1155 RNAs Induced by Heat 1167 OTHER INDUCTIONS OF HSPs 1168 Developmental Inductions .. . ... . . . . . . .. .. .. . ... ...... . .... . .. . . . 1168 Other Inducers 1 1 69 Is There a Common Mechanism? ........ ... 1170 GENOME ORGANIZATION 1172 REGULATION OF THE RESPONSE 1173 Transcription 1173 Translation . . . . .. ..... ......... .. . . .. .. .. .... . .. ...... . . . .. . . . . .. .. .. . .. .. .. . . . . . . . . . 1177 RNA Processing 1178 TOLERANCE TO HEAT AND OTHER FORMS OF STRESS . . . 1179 Heat-Induced Lethality and Thermotolerance . . . . . . . . . . . . . 1179 Phenocopies 1182 CONCLUDING REMARKS 1184

4,655 citations


Journal ArticleDOI
25 Apr 1986-Cell
TL;DR: It is suggested that the disruption of intron processing contributes to heat- induced lethality and developmental abnormalities and that one function of the heat shock proteins is to protect processing from heat-induced disruption.

494 citations


Journal ArticleDOI
07 Mar 1986-Science
TL;DR: A strikingly similar pattern of expression occurs during oogenesis in Drosophila, suggesting that it may be one of the earliest developmental pathways to evolve in eukaryotic cells.
Abstract: Every eukaryotic and prokaryotic organism tested to date synthesizes a small number of heat-shock proteins in response to heat and other forms of stress. A particular pattern of heat-shock gene expression was observed during ascospore development in Saccharomyces: heat-shock proteins hsp26 and hsp84 were strongly induced nor inducible by heat shock. Instead, two proteins related to hsp70 were induced. A strikingly similar pattern of expression occurs during oogenesis in Drosophila, suggesting that it may be one of the earliest developmental pathways to evolve in eukaryotic cells.

283 citations


Journal ArticleDOI
20 Jun 1986-Cell
TL;DR: Surprisingly, the mutations isolated from the yeast Saccharomyces cerevisiae have no detectable effect on the following characteristics: growth rates at various temperatures, in fermentative or in respiratory metabolism, in rich or in minimal media; the acquisition of thermotolerance in log phase or in stationary phase cells; resistance to ethanol; spore development; thermoresistance during sporulation; sporesistance of mature or germinating spores; or survival after long-term storage.

215 citations


Journal ArticleDOI
TL;DR: It is shown that antisense RNAs transcribed from genes that are stably integrated into the genome can be used to inhibit the expression of an endogenous cellular gene.
Abstract: We show that antisense RNAs transcribed from genes that are stably integrated into the genome can be used to inhibit the expression of an endogenous cellular gene. Drosophila tissue culture cells were stably transformed with a gene encoding a heat-inducible RNA complementary to the message for hsp26, one of the small heat shock proteins. These cells produced much less hsp26 after heat shock than did untransformed cells. The inhibition was highly specific: expression of the closely related heat shock proteins hsp22, hsp23, and hsp28 was unaffected. By varying the copy number of the antisense gene, the degree of inhibition was varied over a broad range. Reducing the rate of hsp26 synthesis did not appear to affect the synthesis of any other protein during either heat shock or recovery.

123 citations


Journal ArticleDOI
06 Jun 1986-Cell
TL;DR: It is concluded that the development of ascospores proceeds through the differentiation of functionally distinct subcellular compartments, and several observations suggest that the first set of messages is involved in spore wall synthesis.

29 citations