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Swapan K. Roy

Bio: Swapan K. Roy is an academic researcher from University of Calcutta. The author has contributed to research in topics: Silica gel & Serum albumin. The author has an hindex of 2, co-authored 2 publications receiving 36 citations. Previous affiliations of Swapan K. Roy include Albert Einstein College of Medicine.

Papers
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Journal ArticleDOI
TL;DR: Aminopropyl silica gel may be preferred over conventional organic solid supports for the following reasons: faster flow rate; higher capacity; 3) easier handling; 4) more economical; and 5) lower susceptibility to microbial attack.

24 citations

Journal ArticleDOI
TL;DR: Epoxy silica gel, prepared by reaction of porous silica Gel with γ-glycidoxypropyltriethoxysilane, was coupled with m -aminobenzamidine and the resulting matrix was used for trypsin purification.

12 citations


Cited by
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Journal ArticleDOI
TL;DR: It was found that treatment of gels with dithiothreitol prior to impregnation with silver nitrate results in more reproducible staining patterns that are also qualitatively similar to those obtained with Coomassie blue.

4,022 citations

Journal ArticleDOI
TL;DR: The CO(2) capacity and organic content of all adsorbents decrease after steam treatment under both steam/air and steam/nitrogen conditions, primarily due to structural collapse of the MCF framework, but with additional contributions likely associated with amine degradation during treatment under harsh conditions.
Abstract: Three classes of amine-functionalized mesocellular foam (MCF) materials are prepared and evaluated as CO2 adsorbents. The stability of the adsorbents under steam/air and steam/nitrogen conditions is investigated using a Parr autoclave reactor to simulate, in an accelerated manner, the exposure that such adsorbents will see under steam stripping regeneration conditions at various temperatures. The CO2 capacity and organic content of all adsorbents decrease after steam treatment under both steam/air and steam/nitrogen conditions, primarily due to structural collapse of the MCF framework, but with additional contributions likely associated with amine degradation during treatment under harsh conditions. Treatment with steam/air is found to have stronger effect on the CO2 capacity of the adsorbents compared to steam/nitrogen.

147 citations

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TL;DR: It is demonstrated that GC is a major target in antibody-mediated demyelination and myelin swelling in vitro and not against MBP, the major glycolipid of CNS myelin.

131 citations

Journal ArticleDOI
TL;DR: The AMPPD technique was demonstrated by the detection of proteins from individual photoreceptor outer segments, including proteins constituting approximately 1% of the total, and proved extremely sensitive for immunoblotting.
Abstract: The analysis of proteins from single cells requires techniques of supreme sensitivity. Although radiochemical procedures are capable of detecting small amounts of electrophoretically separated proteins, their sensitivity falls short of that required for routine detection of minor components of single cells. Utilizing the avidin-biotin interaction and the alkaline phosphatase substrate 3-(4-methoxyspiro[1,2-dioxetane-3,2'- tricyclo-[3.3.1.1(3,7)]decan]-4-yl)phenyl phosphate (AMPPD), we have developed an alternative, chemiluminescence-based method for protein detection whose sensitivity exceeds that of other methods. Applying this method to a purified protein, we could detect as little as 63 fg (0.9 amol) of biotinylated bovine serum albumin. The sensitivity of the method was demonstrated by the detection of proteins from individual photoreceptor outer segments, including proteins constituting approximately 1% of the total. Chemiluminescence detection also proved extremely sensitive for immunoblotting: a comparison of five methods for detection of antibody-antigen interactions showed that the AMPPD technique was more sensitive than detection with a colorimetric alkaline phosphatase substrate, 125I-labeled protein A, 125I-labeled anti-mouse IgG, or colloidal gold-conjugated anti-mouse IgG.

93 citations

Journal ArticleDOI
TL;DR: A number of glycosphingolipids, including 10 gangliosides, not previously identified in human plasma have been characterized and are tentatively identified by immunostaining with monoclonal antibodies.

67 citations