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Showing papers by "T.S. Chandra published in 2003"


Journal ArticleDOI
TL;DR: This is the first report of a Clostridium strain growing at 20 degrees C and producing an array of xylanolytic and cellulolytic enzymes, possessing low temperature optima of 20 degreesC, which may facilitate degradation of plant fibre under low-temperature conditions.
Abstract: A Clostridium strain PXYL1 was isolated from a cold-adapted cattle manure biogas digester at 15°C. It could grow at temperatures as low as 5°C up to 50°C with highest specific growth rate at 20°C and is a psychrotroph. It produced extracellular hydrolytic enzymes namely xylanase, endoglucanase, β-xylosidase, β-glucosidase and filter paper cellulase, all of which had maximal activity at 20°C. The induction of xylanase was highest on birch wood xylan (37 IU(mg protein)−1) compared with xylose (1.11 IU(mg protein)−1), cellobiose (1.43 IU(mg protein)−1) and glucose (no activity). The xylanase was thermolabile with a half-life of 30 min at 40°C and 8 min at 50°C but stable for over 2 h at 20°C. The crude enzyme released reducing sugars (1.25 g l−1) from finger millet flour at 20°C, while commercial food-grade xylanases showed no hydrolysis at this temperature. This is the first report of a Clostridium strain growing at 20°C and producing an array of xylanolytic and cellulolytic enzymes, possessing low temperature optima of 20°C, which may facilitate degradation of plant fibre under low-temperature conditions.

48 citations


Journal ArticleDOI
TL;DR: This study defines the conditions (wavelength selection and sensitivity) for the spectrofluorimetric quantification of lipids in situ in the macerated mycelia of this fungus in the presence of intracellular autofluorescent riboflavin without the need to extract the lipids from theMycelia.

11 citations