Author
Takaharu Sakiyama
Other affiliations: University of Tokyo, Massachusetts Institute of Technology, Okayama University
Bio: Takaharu Sakiyama is an academic researcher from Tokyo University of Marine Science and Technology. The author has contributed to research in topics: Adsorption & Swelling. The author has an hindex of 30, co-authored 110 publications receiving 3256 citations. Previous affiliations of Takaharu Sakiyama include University of Tokyo & Massachusetts Institute of Technology.
Topics: Adsorption, Swelling, Thermolysin, Polyelectrolyte, Desorption
Papers published on a yearly basis
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TL;DR: In this article, the authors stress the importance and interesting aspect of protein adsorption on solid surfaces by reviewing findings that have been obtained in recent years, and stress that information on the conformation of the adsorbed protein as well as adsorbing characteristics is essential for a system's performance.
890 citations
TL;DR: A general approach is presented for creating polymer gels that can recognize and capture a target molecule by multiple-point interaction and that can reversibly change their affinity to the target by more than one order of magnitude.
Abstract: A general approach is presented for creating polymer gels that can recognize and capture a target molecule by multiple-point interaction and that can reversibly change their affinity to the target by more than one order of magnitude. The polymers consist of majority monomers that make the gel reversibly swell and shrink and minority monomers that constitute multiple-point adsorption centers for the target molecule. Multiple-point interaction is experimentally proven by power laws found between the affinity and the concentration of the adsorbing monomers within the gels.
180 citations
TL;DR: A polyelectrolyte complex gel was prepared by mixing chitosan and k-carrageenan solutions in the presence of NaCl, and the diameter of the cylindrical gel was measured as discussed by the authors.
Abstract: A polyelectrolyte complex gel was prepared by mixing chitosan and k-carrageenan solutions in the presence of NaCl. To study the effect of ambient pH on the swelling behavior, the diameter of the cylindrical gel(4 mm x 8 mm) immersed in aqueous NaOH, KOH, or HCl solution was measured. In the range of pH 10–12, the diameter increased with time, and the gel reached swelling equilibrium within 6 days. In an NaOH solution of pH 10.5, the maximum swelling occurred, and the volume of the gel at equilibrium was 10.2 times as large as the initial one. At pH below 9 and at pH 13, no swelling was observed. Thus, the swelling of the complex gel prepared in this study was sensitive to a rather narrow range of pH. The swelling equilibrium in the presence of NaCl was also investigated at various pH. © 1993 John Wiley & Sons, Inc.
167 citations
TL;DR: This review focuses on recent advances in immobilization methodol- ogy for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library sys- tems.
Abstract: Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of re- searchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while re- taining structure and native biological function. In this review, we focus on recent advances in immobilization methodol- ogy for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library sys- tems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.
107 citations
TL;DR: It was found that the kind of substituent groups at most positions of the polyheterocycles of methylene blue strongly affects the adsorption behavior, particularly the area occupied by an adsorbed dye molecule, the affinity for the stainless steel surface, and the orientation of the adsorsed dye molecule on the Stainless steel surface.
97 citations
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TL;DR: Chitin is the most abundant natural amino polysaccharide and is estimated to be produced annually almost as much as cellulose, and recent progress in chitin chemistry is quite noteworthy as mentioned in this paper.
Abstract: Chitin is the most abundant natural amino polysaccharide and is estimated to be produced annually almost as much as cellulose. It has become of great interest not only as an underutilized resource, but also as a new functional material of high potential in various fields, and recent progress in chitin chemistry is quite noteworthy. The purpose of this review is to take a closer look at chitin and chitosan applications. Based on current research and existing products, some new and futuristic approaches in this fascinating area are thoroughly discussed.
5,517 citations
TL;DR: From a comprehensive literature review, it was found that some LCAs, in addition to having wide availability, have fast kinetics and appreciable adsorption capacities too.
3,163 citations
TL;DR: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. S. Nagar, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of medicine, Jerusalem 91120, Israel.
Abstract: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. A. S. Nagar,Mohali, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy,Green Biotechnology Research Group, The Special Division for Human Life Technology, National Institute of Advanced Industrial Science andTechnology, 1-8-31 Midorigaoka, Ikeda, Osaka-563-8577, Japan, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of Medicine, Jerusalem 91120, IsraelReceived March 2, 2004
2,570 citations
TL;DR: It is evident from a literature survey of about 185 recently published papers that low-cost adsorbents have demonstrated outstanding removal capabilities for MB, and these include agricultural wastes, industrial solid wastes, biomass, clays minerals and zeolites.
2,493 citations
TL;DR: The exciting successes in taming molecular-level movement thus far are outlined, the underlying principles that all experimental designs must follow, and the early progress made towards utilizing synthetic molecular structures to perform tasks using mechanical motion are highlighted.
Abstract: The widespread use of controlled molecular-level motion in key natural processes suggests that great rewards could come from bridging the gap between the present generation of synthetic molecular systems, which by and large rely upon electronic and chemical effects to carry out their functions, and the machines of the macroscopic world, which utilize the synchronized movements of smaller parts to perform specific tasks. This is a scientific area of great contemporary interest and extraordinary recent growth, yet the notion of molecular-level machines dates back to a time when the ideas surrounding the statistical nature of matter and the laws of thermodynamics were first being formulated. Here we outline the exciting successes in taming molecular-level movement thus far, the underlying principles that all experimental designs must follow, and the early progress made towards utilizing synthetic molecular structures to perform tasks using mechanical motion. We also highlight some of the issues and challenges that still need to be overcome.
2,301 citations