Author
Thierry Bogaert
Bio: Thierry Bogaert is an academic researcher. The author has contributed to research in topics: Genetically modified maize & Bacillus thuringiensis. The author has an hindex of 1, co-authored 1 publications receiving 1351 citations.
Papers
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TL;DR: It is demonstrated that ingestion of double-stranded (ds)RNAs supplied in an artificial diet triggers RNA interference in several coleopteran species, most notably the western corn rootworm Diabrotica virgifera virgifiera LeConte, suggesting that the RNAi pathway can be exploited to control insect pests via in planta expression of a dsRNA.
Abstract: Commercial biotechnology solutions for controlling lepidopteran and coleopteran insect pests on crops depend on the expression of Bacillus thuringiensis insecticidal proteins1,2, most of which permeabilize the membranes of gut epithelial cells of susceptible insects3 However, insect control strategies involving a different mode of action would be valuable for managing the emergence of insect resistance Toward this end, we demonstrate that ingestion of double-stranded (ds)RNAs supplied in an artificial diet triggers RNA interference in several coleopteran species, most notably the western corn rootworm (WCR) Diabrotica virgifera virgifera LeConte This may result in larval stunting and mortality Transgenic corn plants engineered to express WCR dsRNAs show a significant reduction in WCR feeding damage in a growth chamber assay, suggesting that the RNAi pathway can be exploited to control insect pests via in planta expression of a dsRNA
1,545 citations
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TL;DR: This review brings together current knowledge on the uptake mechanisms of dsRNA in insects and the potential of RNAi to affect pest insects, highlighting the achievement of implementing RNAi in insect control with the first successful experiments in transgenic plants and the diversity of successfully tested insect orders/species and target genes.
841 citations
TL;DR: Field outcomes support theoretical predictions that factors delaying resistance include recessive inheritance of resistance, low initial frequency of resistance alleles, abundant refuges of non-Bt host plants and two-toxin Bt crops deployed separately from one-t toxin BT crops.
Abstract: Evolution of resistance in pests can reduce the effectiveness of insecticidal proteins from Bacillus thuringiensis (Bt) produced by transgenic crops We analyzed results of 77 studies from five continents reporting field monitoring data for resistance to Bt crops, empirical evaluation of factors affecting resistance or both Although most pest populations remained susceptible, reduced efficacy of Bt crops caused by field-evolved resistance has been reported now for some populations of 5 of 13 major pest species examined, compared with resistant populations of only one pest species in 2005 Field outcomes support theoretical predictions that factors delaying resistance include recessive inheritance of resistance, low initial frequency of resistance alleles, abundant refuges of non-Bt host plants and two-toxin Bt crops deployed separately from one-toxin Bt crops The results imply that proactive evaluation of the inheritance and initial frequency of resistance are useful for predicting the risk of resistance and improving strategies to sustain the effectiveness of Bt crops
838 citations
TL;DR: Nanosized gold delivered DNA to plant cells while iron oxide based nanosensors detected pesticides at minute levels, assisting the development of precision farming by minimizing pollution and maximizing the value of farming practice.
754 citations
TL;DR: Analysis of more than a decade of global monitoring data reveals that the frequency of resistance alleles has increased substantially in some field populations of Helicoverpa zea, but not in five other major pests in Australia, China, Spain and the United States.
Abstract: Evolution of insect resistance threatens the continued success of transgenic crops producing Bacillus thuringiensis (Bt) toxins that kill pests. The approach used most widely to delay insect resistance to Bt crops is the refuge strategy, which requires refuges of host plants without Bt toxins near Bt crops to promote survival of susceptible pests. However, large-scale tests of the refuge strategy have been problematic. Analysis of more than a decade of global monitoring data reveals that the frequency of resistance alleles has increased substantially in some field populations of Helicoverpa zea, but not in five other major pests in Australia, China, Spain and the United States. The resistance of H. zea to Bt toxin Cry1Ac in transgenic cotton has not caused widespread crop failures, in part because other tactics augment control of this pest. The field outcomes documented with monitoring data are consistent with the theory underlying the refuge strategy, suggesting that refuges have helped to delay resistance.
727 citations
Swedish University of Agricultural Sciences1, University of Exeter2, Commonwealth Scientific and Industrial Research Organisation3, University of Bath4, George Washington University5, Ghent University6, Centre for DNA Fingerprinting and Diagnostics7, University of Copenhagen8, Kansas State University9, University of Montpellier10, Max Planck Society11, University of Warsaw12, University of Georgia13, National Autonomous University of Mexico14, Australian National University15, University of Valencia16, Wageningen University and Research Centre17, Agriculture and Agri-Food Canada18, University of Saskatchewan19, Stockholm University20, Eötvös Loránd University21, University of Tokyo22, National Institute of Advanced Industrial Science and Technology23, Plant & Food Research24, Oregon State University25, Agricultural Research Service26, Leiden University27, University of Manitoba28
TL;DR: Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity and that gene expression in epidermal tissues seems to be most difficult to silence.
698 citations