Author
Thomas J. Burke
Bio: Thomas J. Burke is an academic researcher from Promega. The author has contributed to research in topics: Hot start PCR & Multiple displacement amplification. The author has an hindex of 2, co-authored 3 publications receiving 104 citations.
Papers
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Patent•
03 Aug 1989TL;DR: In this paper, a modified Taq DNA polymerase and methods for its use are described and a modified version of the original Taq polymerase is presented. But the present invention is directed to a modified DNA polymerization.
Abstract: The present invention is directed to a modified Taq DNA polymerase and methods for its use.
81 citations
Patent•
25 Jul 1990
TL;DR: In this article, a modified Taq DNA polymerase and its method for use is described. But the present invention is directed to a modified DNA polymerization and its manufacture and its methods for use.
Abstract: The present invention is directed to a modified Taq DNA polymerase its manufacture and its method for use.
23 citations
Patent•
25 Jul 1990
TL;DR: In this paper, a polymerase d'ADN de Taq modifie, sa fabrication et sa methode d'utilisation, is presented, which concerne une polymerase.
Abstract: La presente invention concerne une polymerase d'ADN de Taq modifie, sa fabrication et sa methode d'utilisation.
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06 Aug 1990TL;DR: In this article, the authors used the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide fragments for detection.
Abstract: The present invention is directed to a process of detecting a target nucleic acid using labeled oligonucleotides. This process uses the 5' to 3' nuclease activity of a nucleic acid polymerase to cleave annealed labeled oligonucleotide from hybridized duplexes and release labeled oligonucleotide fragments for detection. This process is easily incorporated into a PCR amplification assay.
983 citations
Patent•
06 Nov 2000TL;DR: In this article, a high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention of the algorithm.
Abstract: Methods for high speed, high throughput analysis of polynucleotide sequences, and apparatuses with which to carry out the methods are provided in the invention.
697 citations
Patent•
29 Nov 1991
TL;DR: In this paper, an instrument for performing highly accurate PCR employing a sample block in microtiter tray format is described. But the sample block has local balance and local symmetry, and the control software includes diagnostics.
Abstract: An instrument for performing highly accurate PCR employing a sample block in microtiter tray format. The sample block has local balance and local symmetry. A three zone film heater controlled by a computer and ramp cooling solenoid valves also controlled by the computer for gating coolant flow through the block controls the block temperature. Constant bias cooling is used for small changes. Sample temperature is calculated instead of measured. A platen deforms plastic caps to apply a minimum acceptable threshold force for seating the tubes and thermally isolates them. A cover isolates the block. The control software includes diagnostics. An install program tests and characterizes the instrument. A new user interface is used. Disposable, multipiece plastic microtiter trays to give individual freedom to sample tubes are taught.
426 citations
Patent•
12 Nov 2004TL;DR: In this article, a polynucleotide sequence is sequenced by stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion, which is called stopping the cycle.
Abstract: The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion.
415 citations
Patent•
09 May 1997TL;DR: Processes and kits for simultaneously amplifying and sequencing nucleic acid molecules, and performing high throughput DNA sequencing are described in this article, where the authors also present a high throughput sequencing algorithm.
Abstract: Processes and kits for simultaneously amplifying and sequencing nucleic acid molecules, and performing high throughput DNA sequencing are described.
363 citations