Author
Thomas W. Myers
Bio: Thomas W. Myers is an academic researcher from Hoffmann-La Roche. The author has contributed to research in topics: DNA polymerase & Polymerase. The author has an hindex of 16, co-authored 29 publications receiving 1015 citations.
Papers
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Patent•
22 Dec 1989
TL;DR: In this article, the replication and amplification of RNA sequences by thermoactive DNA polymerases is described in a preferred embodiment, in which high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase.
Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided.
283 citations
Patent•
24 May 1995TL;DR: In this paper, a method for the replication and amplification of RNA sequences by thermoactive DNA polymerases is described. But this method is based on the reverse transcription and nucleic acid amplification in one tube, one enzyme procedure using a thermostable DNA polymerase.
Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided Reagents particularly suited for the methods of the present invention are provided
92 citations
Patent•
20 Jun 1994TL;DR: In this paper, a method for the replication and amplification of RNA sequences by thermoactive DNA polymerases is described. But this method is based on the reverse transcription and nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase.
Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. In a preferred embodiment, high temperature reverse transcription is coupled to nucleic acid amplification in a one tube, one enzyme procedure using a thermostable DNA polymerase. Methods for eliminating carry over contamination of amplifications due to prior reverse transcription reactions are also provided. Reagents particularly suited for the methods of the present invention are provided.
89 citations
TL;DR: These advances in enzymatic nucleic acid amplification strategies continue to provide research and medical communities with an ever-improving arsenal of ways to amplify RNA and DNA.
89 citations
Patent•
17 Jun 1993TL;DR: In this paper, a method for the replication and amplification of RNA sequences by thermoactive DNA polymerases is described, where reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
Abstract: Methods are provided for the replication and amplification of RNA sequences by thermoactive DNA polymerases. Reverse transcription of RNA is catalyzed by, for example, 94 kDa Taq, 62 kDa Taq, and recombinant Tth DNA polymerase. Reverse transcription is coupled to PCR amplification in a one enzyme procedure using a thermostable polymerase.
81 citations
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Patent•
01 Oct 2015
TL;DR: In this article, a system for sample processing is described, which is capable of performing one or more of a sample preparation, sample assay, and detection step using a small volume of sample.
Abstract: Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample.
413 citations
Patent•
20 Apr 2000TL;DR: In this paper, the use of microsphere arrays to detect and quantify a number of nucleic acid reactions has been proposed, such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs).
Abstract: The present invention is directed to methods and compositions for the use of microsphere arrays to detect and quantify a number of nucleic acid reactions. The invention finds use in genotyping, i.e. the determination of the sequence of nucleic acids, particularly alterations such as nucleotide substitutions (mismatches) and single nucleotide polymorphisms (SNPs). Similarly, the invention finds use in the detection and quantification of a nucleic acid target using a variety of amplification techniques, including both signal amplification and target amplification. The methods and compositions of the invention can be used in nucleic acid sequencing reactions as well. All applications can include the use of adapter sequences to allow for universal arrays.
348 citations
Patent•
06 Nov 1992
TL;DR: In this article, the authors considered chromophore-containing polynucleotides having at least two donor chromophores operatively linked to the polynuclotide by linker arms, such that the chromophors are positioned by linkage along the length of the poynucleotide at a donor-donor transfer distance, and at least one fluorescing acceptor chromophorem operatively attached to the polytope by a linker arm.
Abstract: The present invention contemplates chromophore-containing polynucleotides having at least two donor chromophores operatively linked to the polynucleotide by linker arms, such that the chromophores are positioned by linkage along the length of the polynucleotide at a donor-donor transfer distance, and at least one fluorescing acceptor chromophore operatively linked to the polynucleotide by a linker arm, such that the fluorescing acceptor chromophore is positioned by linkage at a donor-acceptor transfer distance from at least one of the donor chromophores, to form a photonic structure for collecting photonic energy and transferring the energy to an acceptor chromophore, and methods using the photonic structures.
338 citations
Patent•
23 Apr 1998
TL;DR: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as PCIGF and transcribed from prostate tissue, is described in this paper, which are useful for detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, such as prostate cancer.
Abstract: A set of contiguous and partially overlapping cDNA sequences and polypeptides encoded thereby, designated as PCIGF and transcribed from prostate tissue, is described. These sequences are useful for the detecting, diagnosing, staging, monitoring, prognosticating, in vivo imaging, preventing or treating, or determining the predisposition of an individual to diseases and conditions of the prostate, such as prostate cancer. Also provided are antibodies which specifically bind to PCIGF-encoded polypeptide or protein, and agonists or inhibitors which prevent action of the tissue-specific PCIGF polypeptide, which molecules are useful for the therapeutic treatment of prostate diseases, tumors or metastases.
264 citations
Patent•
12 Jul 2002TL;DR: In this article, a variety of multiplexing methods used to amplify and/or genotype a different sample set simultaneously are described. But they do not address the problem of genotype selection.
Abstract: The invention is directed to a variety of multiplexing methods used to amplify and/or genotype a variety of samples simultaneously.
250 citations