T
Thorsten M. Schlaeger
Researcher at Boston Children's Hospital
Publications - 88
Citations - 11343
Thorsten M. Schlaeger is an academic researcher from Boston Children's Hospital. The author has contributed to research in topics: Induced pluripotent stem cell & Embryonic stem cell. The author has an hindex of 40, co-authored 77 publications receiving 10463 citations. Previous affiliations of Thorsten M. Schlaeger include Brigham and Women's Hospital & Howard Hughes Medical Institute.
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Journal ArticleDOI
Highly efficient reprogramming to pluripotency and directed differentiation of human cells with synthetic modified mRNA
Luigi Warren,Philip D. Manos,Philip D. Manos,Tim Ahfeldt,Tim Ahfeldt,Yuin-Han Loh,Hu Li,Hu Li,Frank H. Lau,Wataru Ebina,Pankaj Mandal,Zachary D. Smith,Alexander Meissner,Alexander Meissner,George Q. Daley,Andrew S. Brack,James J. Collins,James J. Collins,James J. Collins,Chad A. Cowan,Thorsten M. Schlaeger,Thorsten M. Schlaeger,Derrick J. Rossi +22 more
TL;DR: It is shown that this approach can reprogram multiple human cell types to pluripotency with efficiencies that greatly surpass established protocols and represents a safe, efficient strategy for somatic cell reprogramming and directing cell fate that has broad applicability for basic research, disease modeling, and regenerative medicine.
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Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts.
Akiko Doi,In-Hyun Park,Bo Wen,Peter Murakami,Martin J. Aryee,Rafael A. Irizarry,Brian R. Herb,Christine Ladd-Acosta,Junsung Rho,Sabine Loewer,Justine D. Miller,Thorsten M. Schlaeger,George Q. Daley,Andrew P. Feinberg +13 more
TL;DR: Substantial hypermethylation and hypomethylation of cytosine-phosphate-guanine island shores in nine human iPS cell lines as compared to their parental fibroblasts are found, suggesting two mechanisms for epigenetic reprogramming in iPS cells and cancer.
Journal ArticleDOI
Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells
Sabine Loewer,Moran N. Cabili,Moran N. Cabili,Mitchell Guttman,Yuin-Han Loh,Kelly Thomas,Kelly Thomas,In-Hyun Park,Manuel Garber,Matthew Curran,Tamer T. Onder,Suneet Agarwal,Philip D. Manos,Philip D. Manos,Sumon Datta,Sumon Datta,Eric S. Lander,Eric S. Lander,Thorsten M. Schlaeger,Thorsten M. Schlaeger,George Q. Daley,John L. Rinn,John L. Rinn +22 more
TL;DR: In this paper, the authors characterized the transcriptional reorganization of large intergenic non-coding RNAs (lincRNAs) that occurs upon derivation of human iPSCs and identified numerous lincRNA whose expression is linked to pluripotency.
Large intergenic non-coding RNA-RoR modulates reprogramming of human induced pluripotent stem cells
Sabine Loewer,Moran N. Cabili,Moran N. Cabili,Mitchell Guttman,Yuin-Han Loh,Kelly Thomas,Kelly Thomas,In-Hyun Park,Manuel Garber,Matthew Curran,Tamer T. Onder,Suneet Agarwal,Philip D. Manos,Philip D. Manos,Sumon Datta,Sumon Datta,Eric S. Lander,Eric S. Lander,Thorsten M. Schlaeger,Thorsten M. Schlaeger,George Q. Daley,John L. Rinn,John L. Rinn +22 more
TL;DR: It is found that one such lincRNA (lincRNA-RoR) modulates reprogramming, thus providing a first demonstration for critical functions of lincRNAs in the derivation of pluripotent stem cells.
Journal ArticleDOI
Live cell imaging distinguishes bona fide human iPS cells from partially reprogrammed cells
Elayne M. Chan,Sutheera Ratanasirintrawoot,Sutheera Ratanasirintrawoot,In-Hyun Park,Philip D. Manos,Philip D. Manos,Yuin-Han Loh,Hongguang Huo,Hongguang Huo,Justine D. Miller,Justine D. Miller,Odelya Hartung,Odelya Hartung,Junsung Rho,Tan A. Ince,George Q. Daley,Thorsten M. Schlaeger,Thorsten M. Schlaeger +17 more
TL;DR: Using serial live imaging of human fibroblasts undergoing reprogramming to identify distinct colony types that morphologically resemble embryonic stem cells yet differ in molecular phenotype and differentiation potential, it is determined that only one colony type represents true iPS cells, whereas the others represent reprograming intermediates.