Timothy K. Lee

TL;DR: Three single-chain antigen-binding proteins are novel recombinant polypeptides, composed of an antibody variable light-chain amino acid sequence tethered to a variable heavy-chain sequence (VH) by a designed peptide that links the carboxyl terminus of the VL sequence to the amino terminusof the VH sequence.

TL;DR: In this paper, the authors used high-throughput microfluidic cell culture and fluorescence microscopy, quantitative gene expression analysis and mathematical modelling to investigate how single mammalian cells respond to different concentrations of the signalling molecule tumour-necrosis factor (TNF)-a, and relay information to the gene expression programs by means of the transcription factor nuclear factor (NF)-kB.

TL;DR: It is shown that mammalian cells can create “noisy” environments to produce diversified responses to stimuli and be linked to a secondary paracrine signal secreted at low concentrations, such that not all cells undergo a second round of NF-κB activation.

TL;DR: Two software packages, Morphometrics and BlurLab, that together enable automated, computationally efficient, unbiased identification of cells and morphological features are introduced and suggest potential functions for unknown genes and differences in modes of action of antibiotics are suggested.

TL;DR: The ultrafast daughter cell separation in S. aureus appears to be driven by accumulation of stress in the peripheral ring and exhibits hallmarks of mechanical crack propagation.