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Showing papers by "Tom J. Mabry published in 1996"


Journal ArticleDOI
TL;DR: Based on spectroscopic evidence, including a series of 2D-NMR analyses, the structure of the new triterpene is assigned as 24-des-isopropyl-7-ene-23-one-9,19; 16,24-dicycloart-3 beta,11 beta,16 alpha,24 alpha-tetraol 3-O-beta-D-xylopryanoside, named here as neocimiside

13 citations


Journal ArticleDOI
TL;DR: A sulfated flavonol glycoside isorhamnetin 3-O-(4″-sulfatyl-rutinoside) (1) was isolated from the aerial parts of Zygophyllum dumosum, and its structure was determined by spectroscopic analysis and comparison with data for isorHAMnetin3-O(2) rutineside (2), a compound also obtained from the same plant as mentioned in this paper.
Abstract: A new sulfated flavonol glycoside isorhamnetin 3-O-(4″-sulfatyl-rutinoside) (1) was isolated from the aerial parts of Zygophyllum dumosum, and its structure was determined by spectroscopic analysis and comparison with data for isorhamnetin 3-O-rutinoside (2), a compound also obtained from the same plant.

9 citations



Journal ArticleDOI
TL;DR: Pokeweed and endod produce ribosome-inactivating proteins which are sequestered in leaf cell walls which display strong antiviral activity, and protocols to isolate protoplasts from suspension culture cells and leaves were developed to study the antiviral mechanism.
Abstract: Pokeweed (Phytolacca americana L.) and endod (P. dodecandra L'Herit) produce ribosome-inactivating proteins which are sequestered in leaf cell walls. These proteins display strong antiviral activity. To aid in studying the antiviral mechanism, we developed protocols to isolate protoplasts from suspension culture cells and leaves. Ninety-five percent of pokeweed or endod culture cells were converted to protoplasts using 2% cellulase, 0.25% pectinase, 0.2 M mannitol, 2% sucrose, 15 mM CaCl2 Murashige and Skoog salts, pH 5.7. Viability was >85% after 24 h. Culture-derived protoplasts were purified by centrifugation through a 15% sucrose pad. Protoplasts collected from the supernatant were then pelleted in 0.3 M mannitol. Pokeweed leaves provided respectable yields (4×10(6) protoplasts/g f w) of partially-purified viable protoplasts when digested in solution containing 1% cellulase, 0.2% Pectolyase, 0.4 M mannitol, CPW salts, 0.5 mM MES, pH 5.6. We were unable to completely separate cell debris from mesophyll protoplasts, which were small and easily damaged by centrifugation. Endod leaves were found to be resilient to several digestion enzymes tested.

5 citations



Book ChapterDOI
01 Jan 1996
TL;DR: This chapter examines how one biosynthetic pathway, the route leading to flavonoid synthesis, responds to conditions which simulate bacterial infection.
Abstract: The abundance of cacti in arid lands is, at least in part, due to several biochemical and anatomical adaptations that cacti have for minimizing water loss To what degree members of the Cactaceae have evolved defenses against microbial attack or insect feeding is not as well studied In this chapter we will examine how one biosynthetic pathway, the route leading to flavonoid synthesis, responds to conditions which simulate bacterial infection The chemical and biochemical studies have focused on a popular ornamental species, Cephalocereus senilis (old-man-cactus; Fig 1A)