Tom Rijsselaere

TL;DR: The best strategy to prevent AI-transmitted diseases is to use boars that are free of specific pathogens, to monitor the animals and semen regularly, and to maintain very high biosecurity.

TL;DR: Dilution of the semen samples to approximately 50 x 10(6) spermatozoa/ml and an objective lens magnification of 60x, analysing at least 100 spermatozosa, are the technical settings proposed to obtain reliable and objective sperm morphometric measurements by the HTR 12.1 Metrix in canine.

TL;DR: Diluting dog semen samples to 50 x 10(6) ml(-1) with physiological saline solution and scanning 30 frames at a frame rate of 60 frames/s are the set-up parameters proposed to obtain objective and standardized canine semen motility results using the HTR Ceros 12.1.

TL;DR: Sperm cells at the spermiogenic and meiotic stages of development are more susceptible to heat stress, together with subtle changes in sperm head shape, which can be detected by FHA but not by conventional semen analysis.

TL;DR: In this paper, the sperm cells were lost in the supernatant of a dog after being centrifuged at different speeds, such as 180, 720, 1620 and 2880 g, and the sperm motility, progressive motility and membrane integrity were assessed after 3 days of storage.