T
Tomoko Akiyama
Researcher at Hiroshima University
Publications - 8
Citations - 374
Tomoko Akiyama is an academic researcher from Hiroshima University. The author has contributed to research in topics: Gel electrophoresis & Lysostaphin. The author has an hindex of 6, co-authored 8 publications receiving 353 citations.
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Journal ArticleDOI
Identification of endo-beta-N-acetylglucosaminidase and N-acetylmuramyl-L-alanine amidase as cluster-dispersing enzymes in Staphylococcus aureus.
Motoyuki Sugai,Hitoshi Komatsuzawa,Tomoko Akiyama,Yeong-Man Hong,Tadahiro Oshida,Yoichiro Miyake,Takayuki Yamaguchi,Hidekazu Suginaka +7 more
TL;DR: Results strongly suggest that the 51-kDa GL and 62-k da AM are involved in cell separation of daughter cells after cell division.
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Characterization of sodium dodecyl sulfate-stable Staphylococcus aureus bacteriolytic enzymes by polyacrylamide gel electrophoresis.
TL;DR: The effects of chemical and physical factors were determined, and it was shown that iodoacetic acid, Hg2+, and Cibacron Blue 3G-A reduced activity, and an optimum pH for enzyme detection was between 7 and 8.
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Purification and molecular characterization of glycylglycine endopeptidase produced by Staphylococcus capitis EPK1.
Motoyuki Sugai,Tamaki Fujiwara,Tomoko Akiyama,Masaru Ohara,Hitoshi Komatsuzawa,Shinji Inoue,Hidekazu Suginaka +6 more
TL;DR: Protein homology search suggested that ALE-1 and lysostaphin are members of the novel Zn2+ protease family with a homologous 38-amino-acid-long motif, Tyr-X-His-X(11)-Val-X (12/20)-Gly- X(5-6)-His.
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Rapid purification method of lysostaphin for analysis of cell-wall proteins
TL;DR: Lysostaphin was purified from a commercially available specimen by using a dye ligand affinity high-pressure liquid chromatography (HPLC) procedure, which is simple and rapid, with high recovery and useful for preparing analytical grade-lysostaph in order to investigate the cell-wall proteins of staphylococcus species.
Journal ArticleDOI
Detection of a staphylococcal endo-β-N-acetylglucosaminidase using polyacrylamide gels
Motoyuki Sugai,Hitoshi Komatsuzawa,Shinichi Tomita,Tomoko Akiyama,Yoichiro Miyake,Hidekazu Suginaka +5 more
TL;DR: This activity gel method detects in the order of 100 pg of staphylococcal endo- β - N -acetylglucosaminidase and is applicable to renaturable bacteriolytic enzymes.