Author
Toshinori Sato
Other affiliations: Shibaura Institute of Technology, Japan Nuclear Cycle Development Institute, Kyoto University ...read more
Bio: Toshinori Sato is an academic researcher from Fukuoka University. The author has contributed to research in topics: Instruction-level parallelism & Liposome. The author has an hindex of 36, co-authored 325 publications receiving 5111 citations. Previous affiliations of Toshinori Sato include Shibaura Institute of Technology & Japan Nuclear Cycle Development Institute.
Topics: Instruction-level parallelism, Liposome, Adder, Microarchitecture, Cache
Papers published on a yearly basis
Papers
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TL;DR: Aminopolysaccharides such as chitosan and polygalactosamine were used to transfer luciferase plasmid into tumor cells and cationic liposome (lipofectin)-associated gene expression was inhibited by serum, while chitOSan showed resistance to serum.
427 citations
TL;DR: The transfection mechanism was investigated, and it was found that plasmid/chitosan complexes most likely condense to form large aggregates (5-8 microm), which absorb to the cell surface, which absorbs to thecell surface.
321 citations
TL;DR: HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors.
156 citations
26 Mar 2007
TL;DR: Canary logic is proposed as a promising technique that enables the typical-case design and is easier to design than the previously proposed Razor logic by eliminating delayed clock.
Abstract: The deep submicron (DSM) semiconductor technologies make the worst-case design impossible, since they can not provide design margins that it requires. Research directions should go to typical-case design methodologies, where designers are focusing on typical cases rather than worrying about very rare worst cases. In this paper, canary logic is proposed as a promising technique that enables the typical-case design. It is easier to design than the previously proposed Razor logic by eliminating delayed clock. Estimates based on gate-level simulations show that the canary logic achieves average power reduction of 30% by exploiting dynamic variations in circuit delay
131 citations
TL;DR: Observations with a confocal laser scanning microscope indicated that the pDNA/lac-chitosan complexes traversed endocytic compartments more rapidly than the p DNA/chitOSan complex.
119 citations
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TL;DR: The current review of 129 references describes the biological activity of several chitosan derivatives and the modes of action that have been postulated in the literature.
2,615 citations
TL;DR: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. S. Nagar, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of medicine, Jerusalem 91120, Israel.
Abstract: Department of Pharmaceutics, National Institute of Pharmaceutical Education and Research (NIPER), Sector 67, S. A. S. Nagar,Mohali, Punjab-160 062, India, Institute of Biochemistry, Faculty of Medicine, Polytechnic University, Via Ranieri 67, IT-60100 Ancona, Italy,Green Biotechnology Research Group, The Special Division for Human Life Technology, National Institute of Advanced Industrial Science andTechnology, 1-8-31 Midorigaoka, Ikeda, Osaka-563-8577, Japan, and Department of Medicinal Chemistry & Natural Products,The Hebrew University of Jerusalem, School of Pharmacy-Faculty of Medicine, Jerusalem 91120, IsraelReceived March 2, 2004
2,570 citations
TL;DR: The chemical structure and relevant biological properties of chitosan for regenerative medicine have been summarized as well as the methods for the preparation of controlled drug release devices and their applications.
2,312 citations
TL;DR: Two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells.
Abstract: The development of nonviral vectors for safe and efficient gene delivery has been gaining considerable attention recently. An ideal nonviral vector must protect the gene against degradation by nuclease in the extracellular matrix, internalize the plasma membrane, escape from the endosomal compartment, unpackage the gene at some point and have no detrimental effects. In comparison to viruses, nonviral vectors are relatively easy to synthesize, less immunogenic, low in cost, and have no limitation in the size of a gene that can be delivered. Significant progress has been made in the basic science and applications of various nonviral gene delivery vectors; however, the majority of nonviral approaches are still inefficient and often toxic. To this end, two nonviral gene delivery systems using either biodegradable poly(D,Llactide-co-glycolide) (PLG) nanoparticles or cell penetrating peptide (CPP) complexes have been designed and studied using A549 human lung epithelial cells. PLG nanoparticles were optimized for gene delivery by varying particle surface chemistry using different coating materials that adsorb to the particle surface during formation. A variety of cationic coating materials were studied and compared to more conventional surfactants used for PLG nanoparticle fabrication. Nanoparticles (~200 nm) efficiently encapsulated plasmids encoding for luciferase (80-90%) and slowly released the same for two weeks. After a delay, moderate levels of gene expression appeared at day 5 for certain positively charged PLG particles and gene expression was maintained for at least two weeks. In contrast, gene expression mediated by polyethyleneimine (PEI) ended at day 5. PLG particles were also significantly less
2,189 citations
TL;DR: This review surveys select works of key groups in Asia developing chitin and chitosan materials for implantable biomedical applications and finds Japan has been the undisputed leader, but other Asian nations, namely Korea, Singapore, Taiwan and Thailand have also made notable contributions.
1,569 citations