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Ursula Rinas

Bio: Ursula Rinas is an academic researcher from Leibniz University of Hanover. The author has contributed to research in topics: Inclusion bodies & Escherichia coli. The author has an hindex of 50, co-authored 149 publications receiving 7763 citations. Previous affiliations of Ursula Rinas include California Institute of Technology & University of Regensburg.


Papers
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Journal ArticleDOI
Herman Jan Pel1, Johannes H. de Winde2, Johannes H. de Winde1, David B. Archer3, Paul S. Dyer3, Gerald Hofmann4, Peter J. Schaap5, Geoffrey Turner6, Ronald P. de Vries7, Richard Albang8, Kaj Albermann8, Mikael Rørdam Andersen4, Jannick Dyrløv Bendtsen9, Jacques A.E. Benen5, Marco A. van den Berg1, Stefaan Breestraat1, Mark X. Caddick10, Roland Contreras11, Michael Cornell12, Pedro M. Coutinho13, Etienne Danchin13, Alfons J. M. Debets5, Peter J. T. Dekker1, Piet W.M. van Dijck1, Alard Van Dijk1, Lubbert Dijkhuizen14, Arnold J. M. Driessen14, Christophe d'Enfert15, Steven Geysens11, Coenie Goosen14, Gert S.P. Groot1, Piet W. J. de Groot16, Thomas Guillemette17, Bernard Henrissat13, Marga Herweijer1, Johannes Petrus Theodorus Wilhelmus Van Den Hombergh1, Cees A. M. J. J. van den Hondel18, René T. J. M. van der Heijden19, Rachel M. van der Kaaij14, Frans M. Klis16, Harrie J. Kools5, Christian P. Kubicek, Patricia Ann van Kuyk18, Jürgen Lauber, Xin Lu, Marc J. E. C. van der Maarel, Rogier Meulenberg1, Hildegard Henna Menke1, Martin Mortimer10, Jens Nielsen4, Stephen G. Oliver12, Maurien M.A. Olsthoorn1, K. Pal5, K. Pal20, Noël Nicolaas Maria Elisabeth Van Peij1, Arthur F. J. Ram18, Ursula Rinas, Johannes Andries Roubos1, Cornelis Maria Jacobus Sagt1, Monika Schmoll, Jibin Sun, David W. Ussery4, János Varga20, Wouter Vervecken11, Peter J.J. Van De Vondervoort18, Holger Wedler, Han A. B. Wösten7, An-Ping Zeng, Albert J. J. van Ooyen1, Jaap Visser, Hein Stam1 
TL;DR: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid, and the sequenced genome revealed a large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors.
Abstract: The filamentous fungus Aspergillus niger is widely exploited by the fermentation industry for the production of enzymes and organic acids, particularly citric acid. We sequenced the 33.9-megabase genome of A. niger CBS 513.88, the ancestor of currently used enzyme production strains. A high level of synteny was observed with other aspergilli sequenced. Strong function predictions were made for 6,506 of the 14,165 open reading frames identified. A detailed description of the components of the protein secretion pathway was made and striking differences in the hydrolytic enzyme spectra of aspergilli were observed. A reconstructed metabolic network comprising 1,069 unique reactions illustrates the versatile metabolism of A. niger. Noteworthy is the large number of major facilitator superfamily transporters and fungal zinc binuclear cluster transcription factors, and the presence of putative gene clusters for fumonisin and ochratoxin A synthesis.

1,161 citations

Journal ArticleDOI
TL;DR: A simple fed-batch process for high cell density cultivation of Escherichia coli TG1 was developed to maintain carbon-limited growth using a defined medium and cell concentrations of 128 and 148 g per 1 dry cell weight were obtained using glucose or glycerol as carbon source.

463 citations

Journal ArticleDOI
TL;DR: The pros and cons of well-established robust refolding techniques such as direct dilution as well as less common ones including diafiltration or chromatographic processes including size exclusion chromatography, matrix- or affinity-based techniques and hydrophobic interaction chromatography are discussed.
Abstract: Recent advances in generating active proteins through refolding of bacterial inclusion body proteins are summarized in conjunction with a short overview on inclusion body isolation and solubilization procedures. In particular, the pros and cons of well-established robust refolding techniques such as direct dilution as well as less common ones such as diafiltration or chromatographic processes including size exclusion chromatography, matrix- or affinity-based techniques and hydrophobic interaction chromatography are discussed. Moreover, the effect of physical variables (temperature and pressure) as well as the presence of buffer additives on the refolding process is elucidated. In particular, the impact of protein stabilizing or destabilizing low- and high-molecular weight additives as well as micellar and liposomal systems on protein refolding is illustrated. Also, techniques mimicking the principles encountered during in vivo folding such as processes based on natural and artificial chaperones and propeptide-assisted protein refolding are presented. Moreover, the special requirements for the generation of disulfide bonded proteins and the specific problems and solutions, which arise during process integration are discussed. Finally, the different strategies are examined regarding their applicability for large-scale production processes or high-throughput screening procedures.

389 citations

Journal ArticleDOI
TL;DR: In this article, the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes, and the characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress.
Abstract: Different species of microorganisms including yeasts, filamentous fungi and bacteria have been used in the past 25 years for the controlled production of foreign proteins of scientific, pharmacological or industrial interest. A major obstacle for protein production processes and a limit to overall success has been the abundance of misfolded polypeptides, which fail to reach their native conformation. The presence of misfolded or folding-reluctant protein species causes considerable stress in host cells. The characterization of such adverse conditions and the elicited cell responses have permitted to better understand the physiology and molecular biology of conformational stress. Therefore, microbial cell factories for recombinant protein production are depicted here as a source of knowledge that has considerably helped to picture the extremely rich landscape of in vivo protein folding, and the main cellular players of this complex process are described for the most important cell factories used for biotechnological purposes.

315 citations

Book ChapterDOI
TL;DR: Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.
Abstract: Strong production of recombinant proteins interferes with cellular processes in many ways. Drainage of precursors and energy urges the cell to readjust metabolic fluxes and enzyme composition, stress responses are induced, and hence the cellular activity is shifted from growth to reorganisation of biomass. This may result in inhibition of growth or low level of product accumulation. The extent of the bacterial stress response is determined by the specific properties of the recombinant protein, and by the rates of transcription and translation. Taking into account the capacities of the host for protein processing and physiological adaptation, production schemes can be developed that enhance volumetric productivity and sustainability of the process.

232 citations


Cited by
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28 Jul 2005
TL;DR: PfPMP1)与感染红细胞、树突状组胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作�ly.
Abstract: 抗原变异可使得多种致病微生物易于逃避宿主免疫应答。表达在感染红细胞表面的恶性疟原虫红细胞表面蛋白1(PfPMP1)与感染红细胞、内皮细胞、树突状细胞以及胎盘的单个或多个受体作用,在黏附及免疫逃避中起关键的作用。每个单倍体基因组var基因家族编码约60种成员,通过启动转录不同的var基因变异体为抗原变异提供了分子基础。

18,940 citations

Journal ArticleDOI
TL;DR: The past 20 years have seen enormous progress in the understanding of the mechanisms used by the enteric bacterium Escherichia coli to promote protein folding, support protein translocation and handle protein misfolding, and these insights have been exploited to tackle the problems of inclusion body formation, proteolytic degradation and disulfide bond generation.
Abstract: The past 20 years have seen enormous progress in the understanding of the mechanisms used by the enteric bacterium Escherichia coli to promote protein folding, support protein translocation and handle protein misfolding. Insights from these studies have been exploited to tackle the problems of inclusion body formation, proteolytic degradation and disulfide bond generation that have long impeded the production of complex heterologous proteins in a properly folded and biologically active form. The application of this information to industrial processes, together with emerging strategies for creating designer folding modulators and performing glycosylation all but guarantee that E. coli will remain an important host for the production of both commodity and high value added proteins.

1,338 citations

Journal ArticleDOI
01 Jun 1992-Yeast
TL;DR: In this article, the authors proposed a method to solve the problem of the "missing link" problem, i.e., "missing links" and "missing connections" problem.
Abstract: 0749-503X/92/060423-66 $38.00

1,280 citations

Journal ArticleDOI
21 Jun 1991-Science
TL;DR: Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates.
Abstract: Application of recombinant DNA methods to restructure metabolic networks can improve production of metabolite and protein products by altering pathway distributions and rates. Recruitment of heterologous proteins enables extension of existing pathways to obtain new chemical products, alter posttranslational protein processing, and degrade recalcitrant wastes. Although some of the experimental and mathematical tools required for rational metabolic engineering are available, complex cellular responses to genetic perturbations can complicate predictive design.

1,173 citations