In recent years, tools for the development of new drugs have been dramatically improved. These include genomic and proteomic research, numerous biophysical methods, combinatorial chemistry and screening technologies. In addition, early ADMET studies are employed in order to significantly reduce the failure rate in the development of drug candidates. As a consequence, the lead finding, lead optimization and development process has gained marked enhancement in speed and efficiency. In parallel to this development, major pharma companies are increasingly outsourcing many components of drug discovery research to biotech companies. All these measures are designed to address the need for a faster time to market. New screening methodologies have contributed significantly to the efficiency of the drug discovery process. The conventional screening of single compounds or compound libraries has been dramatically accelerated by high throughput screening methods. In addition, in silico screening methods allow the evaluation of virtual compounds. A wide range of new lead finding and lead optimization opportunities result from novel screening methods by NMR, which are the topic of this review article.

Drug Discovery Today

Noble metal nanoparticle-based localized surface plasmon resonance (LSPR) is an advanced and powerful label-free biosensing technique which is well-known for its high sensitivity to the surrounding refractive index change in the local environment caused by the biomolecular interactions around the sensing area. The characteristics of the LSPR effect in such sensors are highly dependent on the size, shape and nature of the material properties of the metallic nanoparticles considered. Among the various types of metallic nanoparticles used in studies employing the LSPR technique, the use of gold nanorods (GNRs) has attracted particular attention for the development of sensitive LSPR biosensors, this arising from the unique and intriguing optical properties of the material. This paper provides a detailed review of the key underpinning science for such systems and of recent progress in the development of a number of LSPR-based biosensors which use GNR as the active element, including an overview of the sensing principle, the synthesis of GNRs, the fabrication of a number of biosensors, techniques for surface modification of GNRs and finally their performance in several biosensing applications. The review ends with a consideration of key advances in GNR-based LSPR sensing and prospects for future research and advances for the development of the GNR-based LSPR biosensors.

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Gold nanorod-based localized surface plasmon resonance biosensors: A review

This paper presents a brief overview of the technologies used to implement surface plasmon resonance (SPR) effects into fiber-optic sensors for chemical and biochemical applications and a survey of results reported over the last ten years. The performance indicators that are relevant for such systems, such as refractometric sensitivity, operating wavelength, and figure of merit (FOM), are discussed and listed in table form. A list of experimental results with reported limits of detection (LOD) for proteins, toxins, viruses, DNA, bacteria, glucose, and various chemicals is also provided for the same time period. Configurations discussed include fiber-optic analogues of the Kretschmann–Raether prism SPR platforms, made from geometry-modified multimode and single-mode optical fibers (unclad, side-polished, tapered, and U-shaped), long period fiber gratings (LPFG), tilted fiber Bragg gratings (TFBG), and specialty fibers (plastic or polymer, microstructured, and photonic crystal fibers). Configurations involving the excitation of surface plasmon polaritons (SPP) on continuous thin metal layers as well as those involving localized SPR (LSPR) phenomena in nanoparticle metal coatings of gold, silver, and other metals at visible and near-infrared wavelengths are described and compared quantitatively.

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Review of plasmonic fiber optic biochemical sensors: improving the limit of detection.

Recent advances in polyaniline based biosensors

■ CONTENTS Books, Reviews, and Articles of General Interest 488 Sensors for (Dissolved) Gases and Vapors 489 Hydrogen 489 Hydrocarbons 490 Oxygen 491 Ammonia 493 Carbon Dioxide 494 Nitrogen Oxides 494 Vapors of Organic Solvents 495 Sensors for Humidity, Water Fractions, Hydrogen Peroxide, and Hydrazine 495 Humidity 495 Water Fractions 496 Hydrogen Peroxide and Hydrazine 496 Sensors for pH Values, Ions, and Salinity 496 pH Values 496 Ions 497 Salinity and Ionic Strength 499 Sensors for Organic Species 499 Biosensors 500 Immunosensors 500 PNA-Based Biosensors (DNA, Aptamers) 501 Other Affinity Sensors 501 Enzymatic Biosensors 502 Whole Cell Sensors 502 Advanced Optical Sensing Schemes and Materials 503 Author Information 505 Corresponding Author 505 Notes 505 Biographies 505 Acknowledgments 505 References 505

Fiber-Optic Chemical Sensors and Biosensors (2008–2012)

Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor.

This study presents design and development of evanescent wave absorbance based U-bent plastic optical fiber (POF) probe with high sensitivity and its applications to refractive index (RI) sensing. The probes were fabricated by a controlled decladding procedure to remove the fluorinated polymer without damaging the poly methyl methacrylate (PMMA) core and a simple and scalable fabrication technique to obtain POF U-bent probes of desired geometry. U-bent probes of fiber diameter from 250 to 1000 μm were fabricated and optimum bend diameter for each fiber diameter was investigated. The sensitivity was found to be maximum when the bend diameter of the probe varies from 2 to 3 times the fiber diameter. Probes with 500 μm core and 1.25 mm bend diameter showed highest sensitivity (5.57Δ A 560 nm /ΔRIU) in the visible region to RI changes from 1.33 to 1.47 with a resolution better than 1 milli RI units. Furthermore, U-bent probes were amine functionalized and coated with gold nanoparticles to obtain a localized surface plasmon resonance (LSPR) based RI sensor that has an 8-fold improvement in RI sensitivity, hence extending their applicability to plasmonic biosensing.

Development of LSPR based U-bent plastic optical fiber sensors

Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength.

The refractive index (RI) sensitivity of a localized surface plasmon resonance (LSPR)-based fiber-optic probes is dependent on surface coverage of gold nanoparticles (GNP), fiber core diameter, and probe geometry. For U-bent LSPR fiber-optic probes, which demonstrated an order higher absorption sensitivity over straight probes, bend diameter and probe length may also have a significant influence on the sensitivity. This study on U-bent fiber-optic LSPR probes is aimed at optimizing these parameters to obtain highest possible RI sensitivity. RI sensitivity increases linearly as a function of surface coverage of GNP in the range of 2–22 %. U-bent fiber-optic probes made of 200-, 400-, and 600-μm fiber core diameter show optimum bend diameter value as ∼1.4 mm. In addition, RI sensitivity is almost the same irrespective of fiber core diameter demonstrating flexibility in choice of the fiber and ease in optical coupling. The length of the probe preceding and succeeding the bend region has significantly less influence on RI sensitivity allowing miniaturization of these probes. In addition to these experimental studies, we present a theoretical analysis to understand the relative contribution of evanescent wave absorbance of GNP and refractive losses in the fiber due to GNP, towards the RI sensitivity.

Optimal Design for U-bent Fiber-optic LSPR Sensor Probes

Rapid and low-cost diagnosis of COVID-19 is essential to identify the infected subjects, particularly the asymptomatic cases, primarily to arrest the spread of the disease through local transmission Antibody-based chromatographic serological tests, as an alternative to RT-PCR, offer only limited help due to high false positives We propose to exploit our field-deployable/portable plasmonic fiber-optic absorbance biosensor (P-FAB) platform for one-step, wash-free detection of SARS-CoV-2 virus particles directly in saliva sample with minimal sample pre-processing

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V. V. R. Sai

Papers

Novel U-bent fiber optic probe for localized surface plasmon resonance based biosensor.

Development of LSPR based U-bent plastic optical fiber sensors

Evanescent wave absorbance based fiber optic biosensor for label-free detection of E. coli at 280 nm wavelength.

Optimal Design for U-bent Fiber-optic LSPR Sensor Probes

P-FAB: A Fiber-Optic Biosensor Device for Rapid Detection of COVID-19