V
Vassili N. Lazarev
Researcher at Moscow Institute of Physics and Technology
Publications - 91
Citations - 1372
Vassili N. Lazarev is an academic researcher from Moscow Institute of Physics and Technology. The author has contributed to research in topics: Gene & Chlamydia trachomatis. The author has an hindex of 15, co-authored 74 publications receiving 1004 citations. Previous affiliations of Vassili N. Lazarev include Federal Biomedical Agency & Peninsular Malaysia.
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Journal ArticleDOI
Isolation of exosomes by differential centrifugation: Theoretical analysis of a commonly used protocol.
M. A. Livshits,M. A. Livshits,Elena Khomyakova,Evgeniy G. Evtushenko,Vassili N. Lazarev,Nikolay A. Kulemin,Svetlana E. Semina,Edward V. Generozov,Vadim M. Govorun +8 more
TL;DR: To simplify this “cut-off”-size-based adjustment of centrifugation protocol for any rotor, a web-calculator is developed and measured by the nanoparticle tracking analysis (NTA) technique, the concentration and size distribution of the vesicles after centrifugations agree with those theoretically expected.
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Mutations in a 23S rRNA Gene of Chlamydia trachomatis Associated with Resistance to Macrolides
O. Y. Misyurina,E. V. Chipitsyna,Y. P. Finashutina,Vassili N. Lazarev,T. A. Akopian,A. M. Savicheva,Vadim M. Govorun +6 more
TL;DR: All the isolates had mixed populations of bacteria that did and did not carry 23S rRNA gene mutations, and four isolates were resistant to all the antibiotics and had the mutations A2058C and T2611C in the23S r RNA gene.
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Complete Genome and Proteome of Acholeplasma laidlawii
Vassili N. Lazarev,Vassili N. Lazarev,S. A. Levitskii,S. A. Levitskii,Yu. I. Basovskii,M. M. Chukin,T. A. Akopian,V. V. Vereshchagin,E. S. Kostrjukova,Galina Yu Kovaleva,Marat D. Kazanov,Dmitry B. Malko,Alexey G. Vitreschak,Natalia V. Sernova,Mikhail S. Gelfand,Mikhail S. Gelfand,Irina A. Demina,Marina V. Serebryakova,M. A. Galyamina,N. N. Vtyurin,S. I. Rogov,Dmitry G. Alexeev,V. G. Ladygina,Vadim M. Govorun,Vadim M. Govorun +24 more
TL;DR: The complete genome sequence and proteogenomic map for Acholeplasma laidlawii PG-8A shows a significant capability for the regulation of gene expression and mutagenic response to stress, and candidate phosphorylated proteins were found, which implies the presence of active signaling pathways.
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Proteins and peptides of the salivary gland secretion of medicinal leeches Hirudo verbana, H. medicinalis, and H. orientalis.
I. P. Baskova,E. S. Kostrjukova,M. A. Vlasova,O. V. Kharitonova,S. A. Levitskiy,L. L. Zavalova,Sergei A. Moshkovskii,Vassili N. Lazarev +7 more
TL;DR: Comparison of detected molecular masses with those of six known biologically active compounds produced by medicinal leeches revealed their uneven distribution in SGS of each of the three leech species, which opens prospects for using certain species of medicinal leech for targeted therapy of various pathologies.
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Induced expression of melittin, an antimicrobial peptide, inhibits infection by Chlamydia trachomatis and Mycoplasma hominis in a HeLa cell line.
Vassili N. Lazarev,T. M. Parfenova,S.K Gularyan,O.Yu Misyurina,T. A. Akopian,Vadim M. Govorun +5 more
TL;DR: The effect of an amphipathic antimicrobial peptide, melittin, expressed within recombinant plasmid vectors, on infection with urogenital pathogens Chlamydia trachomatis and Mycoplasma hominis in HeLa cell culture is dealt with.