Author
Vernon M. Ingram
Other affiliations: Harvard University, University of Cambridge, University College London ...read more
Bio: Vernon M. Ingram is an academic researcher from Massachusetts Institute of Technology. The author has contributed to research in topics: Hemoglobin & Globin. The author has an hindex of 50, co-authored 156 publications receiving 10672 citations. Previous affiliations of Vernon M. Ingram include Harvard University & University of Cambridge.
Topics: Hemoglobin, Globin, Peptide, Kinase, Phosphorylation
Papers published on a yearly basis
Papers
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TL;DR: A cDNA encoding DNA (cytosine-5)-methyltransferase (DNA MeTase) of mouse cells has been cloned and sequenced and shows striking similarities to bacterial type II DNA cytosine methyltransferases and appears to represent a catalytic methyltransferase domain.
904 citations
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TL;DR: Gene Mutations in Human Haemoglobin: the Chemical Difference Between Normal and Sickle Cell Haemocytes is illustrated.
Abstract: Gene Mutations in Human Haemoglobin: the Chemical Difference Between Normal and Sickle Cell Haemoglobin
826 citations
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TL;DR: A specific chemical difference between the Globins of Normal Human and Sickle-Cell Anaemia Haemoglobin is identified and this difference is related to the ferric content of the haemoglobin molecule.
Abstract: A Specific Chemical Difference Between the Globins of Normal Human and Sickle-Cell Anaemia Haemoglobin
753 citations
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TL;DR: Rapid, dramatic, and reversible increases in histone acetylation in the presence of n-butyrate are described.
Abstract: LEDER and Leder1 have reported that low concentrations of n-butyrate cause Friend erythroleukaemia cells to begin globin synthesis. Apparently n-butyrate can reverse that part of viral transformation which prevents the expression of differentiation in these cells. Prasad and Sinha2 have summarised the effects of n-butyrate on neuroblastoma, HeLa, and other cell types. They and others3–10 have seen reversible inhibition of proliferation, decrease of DNA content, morphological modifications, and increases in the production of specific enzymes, such as adenylate cyclase, alkaline phosphatase, and a sialyltransferase. The present paper describes rapid, dramatic, and reversible increases in histone acetylation in the presence of n-butyrate.
733 citations
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TL;DR: Samples of human haemoglobin from normal adults and from sickle-cell anaemic patients have been heat denatured and then digested with trypsin and “fingerprints” show that the two proteins differ in only one peptide.
597 citations
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01 Jan 196910,262 citations
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TL;DR: It is demonstrated that two recently identified DNA methyltransferases, DnMT3a and Dnmt3b, are essential for de novo methylation and for mouse development and play important roles in normal development and disease.
5,708 citations
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TL;DR: The approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH, which led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis.
Abstract: In 1901 a physician, Archibald Garrod, observed a patient with black urine. He used this simple observation to demonstrate that a single mutant gene can produce a discrete block in a biochemical pathway, which he called an “inborn error of metabolism”. Garrod’s brilliant insight anticipated by 40 years the one gene-one enzyme concept of Beadle and Tatum. In similar fashion the chemist Linus Pauling and the biochemist Vernon Ingram, through study of patients with sickle cell anemia, showed that mutant genes alter the amino acid sequences of proteins. Clearly, many fundamental advances in biology were spawned by perceptive studies of human genetic diseases (1). We began our work in 1972 in an attempt to understand a human genetic disease, familial hypercholesterolemia or FH. In these patients the concentration of cholesterol in blood is elevated many fold above normal and heart attacks occur early in life. We postulated that this dominantly inherited disease results from a failure of end-product repression of cholesterol synthesis. The possibility fascinated us because genetic defects in feedback regulation had not been observed previously in humans or animals, and we hoped that study of this disease might throw light on fundamental regulatory mechanisms. Our approach was to apply the techniques of cell culture to unravel the postulated regulatory defect in FH. These studies led to the discovery of a cell surface receptor for a plasma cholesterol transport protein called low density lipoprotein (LDL) and to the elucidation of the mechanism by which this receptor mediates feedback control of cholesterol synthesis (2,3). FH was shown to be caused by inherited defects in the gene encoding the LDL receptor, which disrupt the normal control of cholesterol metabolism. Study of the LDL receptor in turn led to the understanding of receptor-mediated endocytosis, a genera! process by which cells communicate with each other through internalization of regulatory and nutritional molecules (4). Receptor-mediated endocytosis differs from previously described biochemical pathways because it depends upon the continuous and highly controlled movement of membraneembedded proteins from one cell organelle to another in a process termed
5,488 citations
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TL;DR: The first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules is created, and it is demonstrated that this “Connectivity Map” resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs.
Abstract: To pursue a systematic approach to the discovery of functional connections among diseases, genetic perturbation, and drug action, we have created the first installment of a reference collection of gene-expression profiles from cultured human cells treated with bioactive small molecules, together with pattern-matching software to mine these data. We demonstrate that this "Connectivity Map" resource can be used to find connections among small molecules sharing a mechanism of action, chemicals and physiological processes, and diseases and drugs. These results indicate the feasibility of the approach and suggest the value of a large-scale community Connectivity Map project.
4,429 citations