Veysel Süzerer

Bio: Veysel Süzerer is an academic researcher from Bingöl University. The author has contributed to research in topics: Micropropagation & Shoot. The author has an hindex of 7, co-authored 19 publications receiving 168 citations. Previous affiliations of Veysel Süzerer include Dicle University & Gebze Institute of Technology.

Micropropagation of the pistachio and its rootstocks by temporary immersion system

Abstract: Although several studies have been reported on the micropropagation of the pistachio and its rootstocks, to date none of them had been efficient on the mass production of these plants in bioreactor systems. Thus, the micropropagation of juvenile pistachio shoot tips and nodal buds was investigated in a temporary immersion bioreactor system (RITA®) and on a conventional semi-solid medium. Among the tested immersion conditions, immersion for 24 min every 16 h reduced vitrification and improved proliferation in the pistachio. Interactions were evident in immersion time and frequency in nodal segments. Nodal buds were better than shoot tips as the highest multiple shoot formation was recorded in MS medium containing 4 mg L−1 BA and 0.1 mg L−1 GA3 in RITA®. Although shoot tip necrosis (STN) was observed in shoots proliferated on semi-solid MS medium, such a symptom did not occur in shoots sprouted in the RITA®. Additionally, these optimized conditions were applied to nodal buds of mature male pistachio ‘Atli’ and Pistacia rootstocks (P. khinjuk Stocks and P. atlantica Desf.), and the micropropagation in the bioreactor system, in comparison to the semi-solid medium, was also improved. Furthermore, in vitro rooting of pistachio plantlets, despite the lower range (27.5 %), was also achieved in RITA®. However, rooting was better on semi-solid medium for all tested species (ranged between 50 and 70 %). The results of this study showed that RITA® could be used for the mass propagation of pistachio and its rootstocks, as well as for other woody plant species.

Clonal micropropagation of Pistacia lentiscus L. and assessment of genetic stability using IRAP markers

Abstract: An efficient protocol for clonal micropropagation of selected genotypes of lentisk, Pistacia lentiscus L., which is cultivated for the masticha resin, has been developed using shoot tip explants originating from in vitro seedlings. BA was found to be optimum for shoot morphogenesis in terms of the number and length of shoots among the cytokinins tested for all cloned genotypes, while the highest shoot length was noticed in the presence of 2iP at a rate 4.92 µM. Efficient rooting (94.15 %) was achieved in a medium containing 19.6 µM IBA with the clone II that was superior to the rest of the clones tested. The method developed for plant acclimatization was satisfactory because a high percentage of plant survival (95 %) in the growth room in the clone II was obtained and the regenerated plantlets resumed growth after 4 months. DNAs from mother seedlings and micropropagated plantlets belonging to 6, 9 and 12 times subcultured were isolated and subjected to IRAP analysis in order to evaluate their genetic stability and detect possibly existing variations among in vitro derived plantlets. The mean percentage of similarity calculated by Jaccard’s similarity coefficient ranged from 78 to 86 % in the four genotypes. Although variation was observed among mother plantlets and its regenerants for all of the clones, polymorphic information content value in the range of 0.391–0.405 indicated the presence of reasonable polymorphism within the clones. The presented data confirmed that the clonal propagation of lentisk by using shoot tips could be used for commercial exploitation of the selected genotype.

In vitro conservation and cryopreservation of mature pistachio (Pistacia vera L.) germplasm

Abstract: As genetic erosion of pistachio (Pistacia vera L.) has been occurring in the Mediterranean, Central and West Asia and North Africa, experiments were conducted to conserve two cultivars (‘Atli’ and ‘Siirt’) of mature pistachio germplasm by assessing both medium- and long-term conservation techniques. In medium-term conservation, our results showed that it was feasible to conserve both cultivars in the form of either microshoots or encapsulated shoot apices up to 12 months at 4°C in the dark. As regards long-term conservation, encapsulation-dehydration and droplet-vitrification techniques were assessed for cryopreservation of cold-hardened and osmoprotected shoot apices of mature ‘Atli’ cultivar. Among the methods tested, 13.6% of regrowth was achieved with incubation of explants in the droplets of vitrification solution for 150 min at 0°C followed by direct immersion in liquid nitrogen (LN), rapidly thawed and then cultured on Murashige and Skoog’s (MS) medium containing 1 mg L−1 BA and 0.5 mg L−1 GA3. The developed droplet-vitrification technique appeared as a promising procedure for long-term preservation of shoot apices of mature pistachio germplasm. Moreover, assesment of genetic fidelity by Random Amplified Polymorphic DNA analysis (RAPD) revealed out high levels of genetic stability between donor plant and cryopreserved plants (similarity indexes between 0.959 and 0.973) after they were subcultured for at least 3 months. The detected low level of genetic instability could be due to the toxic effect of PVS2 and regeneration phase. The optimized conservation techniques, especially slow growth storage, could be applied to preserve other Pistacia species.

In vitro micrografting of the almond cultivars "texas", "ferrastar" and "nonpareil"

Abstract: A successful micrografting technique for the almond cultivars (cvs) “Texas”, “Ferrastar” and “Nonpareil” was developed using in vitro germinated seedlings as rootstocks and axenic shoot cultures established from mature tree sources as microscions. In vitro germinated seedlings, which developed 14 days after culturing in the modified Murashige and Skoog (MS) medium, were decapitated and used as rootstock. Shoot culture initiation from three almond cvs (“Texas”, “Ferrastar” and “Nonpareil”) was successfully achieved by culturing mature shoot tips from forced nodal buds, about 4–6 mm, on a modified MS medium containing 1 mg·L -1 benzyl adenin (BA). Slit micrografting on epicotyl and on hypocotyls were equally successful (83.3 % to 100 %). Grafting success was dependent on the rootstock type and lenght of the scion. Grafting success varied between 83.33 % and 100 % depending on the cultivar, when the scion contained 1, 2, and 3 nodes. When almond scions, about 1.5 cm long, were micrografted on germinated seedling and cultured on proliferation medium (PM), the mean shoot length was 19.84 mm, 16.50 mm, 26.93 mm for the cvs “Texas”, “Ferrastar” and “Nonpareil” respectively. Micrografts could be easily cultured on a hormone-free semi-solid MS medium and were potted out after 4 to 6 weeks of culture growth. Rooted micrografted plantlets were successfully acclimatized and transferred to potting mix with 100 % survival. Although low percentages of variation were obtained in tested cvs (3.70 %, 6.25 % and 10.2 % in “Texas”, “Ferrastar” and “Nonpareil”), molecular analysis showed that the developed micrografting technique produces genetically stable plantlets, at least up to 6 months of sub-culturing in cvs “Ferrastar” and “Nonpareil”. The described micrografting technique could be used for rejuvenation of shoot explants of mature elite almond cultivars and it also has potential use in the commercial production of other almond cultivars.

In vitro response of pistachio nodal explants to silver nitrate. Sci Hortic

Abstract: Abstract The effect of silver nitrate on shoot differentiation and shoot growth was examined in order to improve the regeneration efficiency of pistachio (Pistacia vera L. cv. Kirmizi) in vitro. Nodal explants of in vitro-grown seedlings were used to test various concentrations and combinations of 6-benzyladenine (BA), kinetin (KIN), gibberellic acid (GA3) and silver nitrate (AgNO3). Addition of AgNO3 up to 48.0 μM to the culture medium improved the regeneration frequency and shoot growth, and reduced basal callus formation in all regenerated explants. The highest regeneration frequency (100%) was recorded on Murashige and Skoog (MS) medium containing 9.0 μM BA, 0.2 μM GA3 and 24.0 or 48.0 μM AgNO3 in combination. The best proliferation response in terms of both shoot formation and low callus production was obtained in the medium containing a combination of 9.0 μM BA, 0.2 μM GA3 and 12.0 μM AgNO3. Regenerated shoots, coming from three cycles of subculturing in proliferation media, were rooted in half-strength MS medium containing 12.0 μM indole-3-butyric acid (IBA). Well rooted plantlets were acclimatized and eventually established in peat and perlite. The development and optimization of an effective micropropagation protocol that is presented in this paper can give an important contribution to improve the quality of pistachio plants and, as a consequence, of orchard production in Middle East countries.

Mechanisms Underlying Graft Union Formation and Rootstock Scion Interaction in Horticultural Plants.

Abstract: Grafting is a common practice for vegetative propagation and trait improvement in horticultural plants. A general prerequisite for successful grafting and long term survival of grafted plants is taxonomic proximity between the root stock and scion. For the success of a grafting operation, rootstock and scion should essentially be closely related. Interaction between the rootstock and scion involves complex physiological-biochemical and molecular mechanisms. Successful graft union formation involves a series of steps viz., lining up of vascular cambium, generation of a wound healing response, callus bridge formation, followed by vascular cambium formation and subsequent formation of the secondary xylem and phloem. For grafted trees compatibility between the rootstock/scion is the most essential factor for their better performance and longevity. Graft incompatibility occurs on account of a number of factors including of unfavorable physiological responses across the graft union, transmission of virus or phytoplasma and anatomical deformities of vascular tissue at the graft junction. In order to avoid the incompatibility problems, it is important to predict the same at an early stage. Phytohormones, especially auxins regulate key events in graft union formation between the rootstock and scion, while others function to facilitate the signaling pathways. Transport of macro as well as micro molecules across long distances results in phenotypic variation shown by grafted plants, therefore grafting can be used to determine the pattern and rate of recurrence of this transport. A better understanding of rootstock scion interactions, endogenous growth substances, soil or climatic factors needs to be studied, which would facilitate efficient selection and use of rootstocks in the future. Protein, hormones, mRNA and small RNA transport across the junction is currently emerging as an important mechanism which controls the stock/scion communication and simultaneously may play a crucial role in understanding the physiology of grafting more precisely. This review provides an understanding of the physiological, biochemical and molecular basis underlying grafting with special reference to horticultural plants.

Liquid culture systems for in vitro plant propagation

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Evaluation of different temporary immersion systems (BIT ® , BIG, and RITA ® ) in the micropropagation of Vanilla planifolia Jacks

Abstract: The cultivation of Vanilla planifolia is of great economic importance because vanillin, a chemical valued in the food and cosmetics industry, is extracted from its pods. The conventional propagation of this plant is limited by the low viability of its seeds and the very low germination rate. For this reason, in vitro micropropagation techniques using temporary immersion systems (TIS) represent an alternative propagation mechanism. This work assessed three different bioreactor systems in two different micropropagation phases (multiplication and rooting) of V. planifolia: Temporary Immersion Bioreactors (BIT®), Gravity Immersion Bioreactors (BIG), and Recipient for Automated Temporary Immersion (RITA®). A higher number of shoots/explant were observed in the multiplication phase in BIT® systems (18.06 shoots/explant), followed by RITA® (12.77) and BIG (6.83). In the rooting phase, a higher number of longer roots were obtained in BIT® compared with BIG and RITA®. However, higher chlorophyll content was observed in BIG, followed by RITA® and BIT®. A 100% survival was obtained in vitro micropropagated plantlets in BIT®, exceeding the survival rate observed in RITA® and BIG. In general, our findings confirm the utility of BIT® systems in the optimization of the commercial micropropagation of this species. Furthermore, this system reduces the costs associated with the use of RITA® systems.