Vladimir Benes

TL;DR: The Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines target the reliability of results to help ensure the integrity of the scientific literature, promote consistency between laboratories, and increase experimental transparency.

TL;DR: Analysis of the PEST strain of A. gambiae revealed strong evidence for about 14,000 protein-encoding transcripts, and prominent expansions in specific families of proteins likely involved in cell adhesion and immunity were noted.

TL;DR: An SV discovery method that integrates short insert paired-ends, long-range mate-pairs and split-read alignments to accurately delineate genomic rearrangements at single-nucleotide resolution, called DELLY, which enables to ascertain the full spectrum of genomic rearrANGements, including complex events.

TL;DR: The real-time reverse transcription polymerase chain reaction uses fluorescent reporter molecules to monitor the production of amplification products during each cycle of the PCR reaction, which combines the nucleic acid amplification and detection steps into one homogeneous assay and obviates the need for gel electrophoresis to detect amplification products.

TL;DR: The studies indicate that replicative senescence of MSC preparations is a continuous process starting from the first passage onwards, which includes far reaching alterations in phenotype, differentiation potential, global gene expression patterns, and miRNA profiles that need to be considered for therapeutic application of M SC preparations.