Author
W. G. Duncombe
Bio: W. G. Duncombe is an academic researcher from Wellcome Trust. The author has contributed to research in topics: Polyunsaturated fatty acid & Fatty acid synthesis. The author has an hindex of 2, co-authored 2 publications receiving 887 citations.
Papers
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882 citations
TL;DR: The use of labelled acetate for studying the synthesis of long-chain fatty acids in rat adipose tissue in vitro has been examined and it is shown that this is not necessarily due to disturbance of the endogenous rate of fatty acid synthesis by a relatively large amount of acetyl-CoA derived from added acetate.
Abstract: 1. The use of labelled acetate for studying the synthesis of long-chain fatty acids in rat adipose tissue in vitro has been examined, with special reference to the effect of acetate concentration. 2. The incorporation of acetate into fatty acids is proportional to the concentration of acetate in the medium when the latter does not exceed about 10μm. Above this concentration, the relative incorporation becomes progressively less, and reasons for this are discussed. 3. In particular it is shown that this is not necessarily due to disturbance of the endogenous rate of fatty acid synthesis by a relatively large amount of acetyl-CoA derived from added acetate. 4. However, to ensure that the added acetate does not cause such a disturbance its concentration must be kept sufficiently low. For labelled acetate used under present conditions, this concentration should not be more than about 10μm.
11 citations
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TL;DR: A rapid colorimetric method is described for the determination of nonesterified fatty acids in 0.5 ml of plasma and results obtained were in reasonable agreement with those obtained with an established titration method.
895 citations
TL;DR: The optimum conditions for chloroform extraction of FFA from blood or other biological fluids were studied and a degree and rate of extraction similar to those with Dole’s extraction mixture were obtained.
713 citations
TL;DR: In this article, a colorimetric ultramicro method for the determination of serum and tissue fatty acids has been improved in sensitivity and selectivity by extracting cobalt rather than copper soaps by means of a solvent lighter than water instead of chloroform.
664 citations
TL;DR: An expansion of that work, improving the sensitivity and reproducibility of the method, as well as examination of solubilities of the copper soaps as a function of chain length and unsaturation, was described.
Abstract: In 1964, a method was described for the determination of free fatty acids (FFAs) in vegetable oil. This paper describes an expansion of that work, improving the sensitivity and reproducibility of the method, as well as examination of solubilities of the copper soaps as a function of chain length and unsaturation. Involvement of the micellar structure was reviewed. Finally, a procedure is described that permits very rapid determination of FFA at the 2.0–14.0 µmol (0.5–4.0 mg oleic acid) level, and the results with several oils are given. Particular attention was given to evaluation of solvent systems which would extract the copper complexes.
633 citations
563 citations