Wei Guo

Bio: Wei Guo is an academic researcher from University of Manchester. The author has contributed to research in topics: Welding & Heat-affected zone. The author has an hindex of 25, co-authored 86 publications receiving 2488 citations. Previous affiliations of Wei Guo include Harbin Institute of Technology & Northwestern Polytechnical University.

Optical virtual imaging at 50 nm lateral resolution with a white-light nanoscope

Abstract: Lenses are restricted by diffraction to imaging features roughly the size of visible wavelengths. Wang et al. develop a white-light nanoscope that uses optically transparent spherical silica lenses to virtually image, in the far-field, features down to 50 nm resolution.

Label-free super-resolution imaging of adenoviruses by submerged microsphere optical nanoscopy

Abstract: Because of the small sizes of most viruses (typically 5–150 nm), standard optical microscopes, which have an optical diffraction limit of 200 nm, are not generally suitable for their direct observation. Electron microscopes usually require specimens to be placed under vacuum conditions, thus making them unsuitable for imaging live biological specimens in liquid environments. Indirect optical imaging of viruses has been made possible by the use of fluorescence optical microscopy that relies on the stimulated emission of light from the fluorescing specimens when they are excited with light of a specific wavelength, a process known as labeling or self-fluorescent emissions from certain organic materials. In this paper, we describe direct white-light optical imaging of 75-nm adenoviruses by submerged microsphere optical nanoscopy (SMON) without the use of fluorescent labeling or staining. The mechanism involved in the imaging is presented. Theoretical calculations of the imaging planes and the magnification factors have been verified by experimental results, with good agreement between theory and experiment. Researchers have demonstrated a super-resolution imaging technique that allows the direct observation of adenoviruses of 75 nm in size without staining or the use of fluorescent labeling. The approach, reported by Lin Li and co-workers at the University of Manchester, UK, achieves imaging well beyond the diffraction limit by employing glass microspheres submerged in water. A 100-μm-diameter BaTiO3 microsphere is placed on the object being imaged, illuminated with white light. The microsphere captures near-field evanescent waves and converts them via frustrated total internal reflection into propagating far-field waves, which can be imaged by a conventional optical microscope. The researchers also used the method to image 100-nm-spaced periodic structure of a DVD Blu-Ray disk and 50 nm nanopores in a sample of anodic aluminium oxide.

Microsphere-coupled scanning laser confocal nanoscope for sub-diffraction-limited imaging at 25 nm lateral resolution in the visible spectrum.

Abstract: We report a direct optical super-resolution imaging approach with 25 nm (∼λ/17) lateral resolution under 408 nm wavelength illumination by combining fused silica and polystyrene microspheres with a conventional scanning laser confocal microscope (SLCM). The microsphere deposited on the target surface generates a nanoscale central lobe illuminating a sub-diffraction-limited cross-section located on the target surface. The SLCM confocal pinhole isolates the reflected light from the near-field subdiffractive cross-section and suppresses the noises from the side lobe and the far-field paraxial focal point. The structural detail of the subdiffractive cross-section is therefore captured, and the 2D target surface near the bottom of microspheres can be imaged by intensity-based point scanning.

The influences of particle number on hot spots in strongly coupled metal nanoparticles chain.

Abstract: In understanding of the hot spot phenomenon in single-molecule surface enhanced Raman scattering (SM-SERS), the electromagnetic field within the gaps of dimers (i.e., two particle systems) has attracted much interest as it provides significant field amplification over single isolated nanoparticles. In addition to the existing understanding of the dimer systems, we show in this paper that field enhancement within the gaps of a particle chain could maximize at a particle number N>2, due to the near-field coupled plasmon resonance of the chain. This particle number effect was theoretically observed for the gold (Au) nanoparticles chain but not for the silver (Ag) chain. We attribute the reason to the different behaviors of the dissipative damping of gold and silver in the visible wavelength range. The reported effect can be utilized to design effective gold substrate for SM-SERS applications.

Surface-enhanced Raman spectroscopy (SERS): progress and trends

Abstract: Surface-enhanced Raman spectroscopy (SERS) combines molecular fingerprint specificity with potential single-molecule sensitivity. Therefore, the SERS technique is an attractive tool for sensing molecules in trace amounts within the field of chemical and biochemical analytics. Since SERS is an ongoing topic, which can be illustrated by the increased annual number of publications within the last few years, this review reflects the progress and trends in SERS research in approximately the last three years. The main reason why the SERS technique has not been established as a routine analytic technique, despite its high specificity and sensitivity, is due to the low reproducibility of the SERS signal. Thus, this review is dominated by the discussion of the various concepts for generating powerful, reproducible, SERS-active surfaces. Furthermore, the limit of sensitivity in SERS is introduced in the context of single-molecule spectroscopy and the calculation of the 'real' enhancement factor. In order to shed more light onto the underlying molecular processes of SERS, the theoretical description of SERS spectra is also a growing research field and will be summarized here. In addition, the recording of SERS spectra is affected by a number of parameters, such as laser power, integration time, and analyte concentration. To benefit from synergies, SERS is combined with other methods, such as scanning probe microscopy and microfluidics, which illustrates the broad applications of this powerful technique.

A super-oscillatory lens optical microscope for subwavelength imaging

Abstract: The maximum imaging resolution in classical optics is limited to approximately the wavelength of light used, and subwavelength resolution can only be achieved by advanced imaging schemes. The appeal of the super-oscillatory lens optical microscope described here is that it enables subwavelength imaging with, in principle, unlimited resolution using a modified conventional microscope. The past decade has seen an intensive effort to achieve optical imaging resolution beyond the diffraction limit. Apart from the Pendry–Veselago negative index superlens1, implementation of which in optics faces challenges of losses and as yet unattainable fabrication finesse, other super-resolution approaches necessitate the lens either to be in the near proximity of the object or manufactured on it2,3,4,5,6, or work only for a narrow class of samples, such as intensely luminescent7,8 or sparse9 objects. Here we report a new super-resolution microscope for optical imaging that beats the diffraction limit of conventional instruments and the recently demonstrated near-field optical superlens and hyperlens. This non-invasive subwavelength imaging paradigm uses a binary amplitude mask for direct focusing of laser light into a subwavelength spot in the post-evanescent field by precisely tailoring the interference of a large number of beams diffracted from a nanostructured mask. The new technology, which—in principle—has no physical limits on resolution, could be universally used for imaging at any wavelength and does not depend on the luminescence of the object, which can be tens of micrometres away from the mask. It has been implemented as a straightforward modification of a conventional microscope showing resolution better than λ/6.

All-dielectric optical nanoantennas

Abstract: We study in detail a novel type of optical nanoantennas made of high-permittivity low-loss dielectric particles. In addition to the electric resonances, the dielectric particles exhibit very strong magnetic resonances at the nanoscale, that can be employed in the Yagi-Uda geometry for creating highly efficient optical nanoantennas. By comparing plasmonic and dielectric nanoantennas, we demonstrate that all-dielectric nanoantennas may exhibit better radiation efficiency also allowing more compact design.

Advanced Fluorescence Microscopy Techniques—FRAP, FLIP, FLAP, FRET and FLIM

Abstract: Fluorescence microscopy provides an efficient and unique approach to study fixed and living cells because of its versatility, specificity, and high sensitivity. Fluorescence microscopes can both detect the fluorescence emitted from labeled molecules in biological samples as images or photometric data from which intensities and emission spectra can be deduced. By exploiting the characteristics of fluorescence, various techniques have been developed that enable the visualization and analysis of complex dynamic events in cells, organelles, and sub-organelle components within the biological specimen. The techniques described here are fluorescence recovery after photobleaching (FRAP), the related fluorescence loss in photobleaching (FLIP), fluorescence localization after photobleaching (FLAP), Forster or fluorescence resonance energy transfer (FRET) and the different ways how to measure FRET, such as acceptor bleaching, sensitized emission, polarization anisotropy, and fluorescence lifetime imaging microscopy (FLIM). First, a brief introduction into the mechanisms underlying fluorescence as a physical phenomenon and fluorescence, confocal, and multiphoton microscopy is given. Subsequently, these advanced microscopy techniques are introduced in more detail, with a description of how these techniques are performed, what needs to be considered, and what practical advantages they can bring to cell biological research.

Light scattering and surface plasmons on small spherical particles

Abstract: Light scattering by small particles has a long and interesting history in physics. Nonetheless, it continues to surprise with new insights and applications. This includes new discoveries, such as novel plasmonic effects, as well as exciting theoretical and experimental developments such as optical trapping, anomalous light scattering, optical tweezers, nanospasers, and novel aspects and realizations of Fano resonances. These have led to important new applications, including several ones in the biomedical area and in sensing techniques at the single-molecule level. There are additionally many potential future applications in optical devices and solar energy technologies. Here we review the fundamental aspects of light scattering by small spherical particles, emphasizing the phenomenological treatments and new developments in this field. The interaction of light with small spherical particles has long been a topic of interest to researchers. Indeed, understanding many natural phenomena, including rainbows and the solar corona, requires knowledge of how light behaves in such circumstances. Xiaofeng Fan and co-workers from Jilin University in China and Oak Ridge National Laboratory in the USA have now reviewed the physics and applications that arise during the interaction of light with small spherical particles. The researchers describe how Mie theory can be used to describe optical scattering by small dielectric particles, and, in the case of metallic particles, how light excites surface plasmons to generate an optical response featuring asymmetric Fano resonances. In the special case when metallic particles are surrounded by an optical gain medium, plasmons can be amplified; the resulting device is known as a ‘spaser’.