Author
Wei Kunhua
Bio: Wei Kunhua is an academic researcher. The author has contributed to research in topics: Plant tissue culture & Dendrobium gibsonii. The author has an hindex of 1, co-authored 2 publications receiving 7 citations.
Topics: Plant tissue culture, Dendrobium gibsonii
Papers
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Patent•
15 Apr 2015
7 citations
Patent•
11 Nov 2015
TL;DR: In this paper, a tissue culture propagation method for dendrobium gibsonii lindl was proposed, where newly-sprouted tender buds of healthy plants were used as explants and disinfected, and the disinfected explants were put into an MS basic culture medium to be induced to obtain sterile test tube seedlings.
Abstract: The invention provides a tissue culture propagation method for dendrobium gibsonii lindl. The tissue culture propagation method comprises the following steps: (1) taking newly-sprouted tender buds of healthy plants of the dendrobium gibsonii lindl as explants and disinfecting; (2) putting the disinfected explants into an MS basic culture medium to be induced to obtain sterile test tube seedlings; and (3) putting the sterile test tube seedlings into an MS propagation culture medium to perform rapid propagation culture of the test tube seedlings to obtain cluster buds. With the adoption of the culture method provided by the invention, the growth coefficient of the obtained cluster buds of the dendrobium gibsonii lindl can be increased by 20-28 times, high-quality dendrobium gibsonii lindl germchits with a high survival rate can be provided in short time, and a large-scale seedling growing problem of the dendrobium gibsonii lindl is effectively solved.
Cited by
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Patent•
04 May 2016
TL;DR: In this paper, a method for inducing and quickly breeding daphnemezereum seeds is described, which comprises the following steps: (1) taking high-quality DAPHNEMEEREUM seeds as explants and performing disinfection; (2) putting the disinfected explants in an MS minimal medium for culture and induction, and enabling the aseptic buds to be subjected to subculture multiplication culture till multiple buds grow; and (3) shearing the multiple buds into a single bud, and putting the single bud into a strong-seedling
Abstract: Disclosed is a method for inducing and quickly breeding daphnemezereum seeds. The method comprises the following steps: (1) taking high-quality daphnemezereum seeds as explants and performing disinfection; (2) putting the disinfected explants in an MS minimal medium for culture and induction till budding; (3) putting the aseptic buds in an MS minimal medium, and enabling the aseptic buds to be subjected to subculture multiplication culture till multiple buds grow; and (4) shearing the multiple buds into a single bud, and putting the single bud into a strong-seedling rooting medium for culture to obtain a complete sprout with roots. According to the method, the reproduction speed of daphnemezereum sprouts is increased, and the seed original sprouts are not easy to degenerate, so that the quality of sprouts is ensured. The method provides high-quality seed sources for further development of species research, and has bright application prospects.
3 citations
Patent•
01 Apr 2015
TL;DR: In this article, a callus induction and anti-browning method for dracaena cochinchinensis seeds was presented, where the callus obtained by using the method is viridis or cream yellow, high in growing speed and difficult to brown.
Abstract: The invention discloses a callus induction and anti-browning method for dracaena cochinchinensis The method comprises the following steps: (1) using dracaena cochinchinensis seeds as explants for sterilizing; (2) putting the sterilized explants into an MS minimal medium for performing induced budding; (3) putting germ-free shoots into the MS minimal medium for culturing to obtain callus; (4) putting the callus into an MS enrichment medium for culturing to obtain a large amount of callus; and (5) putting the large amount of callus into an MS anti-browning culture medium for culturing to obtain the callus with fluffy texture The callus obtained by using the method is viridis or cream yellow, high in growing speed and difficult to brown, and can produce a large amount of callus with good growth trend and low browning rate by means of induced proliferation; and therefore, high-quality materials are provided for extracting dragon's blood in medical science, and the market requirement can be further met
1 citations
Patent•
05 Jun 2018
TL;DR: In this article, a rapid propagation method for draceana arborea tissue culture is described, which takes steams and lateral buds of dracean arboreas as explants for tissue culture.
Abstract: The invention discloses a rapid propagation method for draceana arborea tissue culture, which takes steams and lateral buds of draceana arborea as explants for tissue culture. A great amount of draceana arborea maintaining excellent female parent properties is obtained by explant pretreatment and disinfection, explant budding induction, rapid propagation, cluster bud strong seedling culture, rooting culture and acclimatization and transplant. The method has the benefits that explant budding induction success rate and transplanting survival rate are increased by adding Tween to an explant disinfectant, rooting percentage of rooting culture is increased by strong seedling culture, and a great amount of the market requirement for draceana arborea is met.
Patent•
14 Aug 2018
TL;DR: In this article, a tabebuia chrysantha tissue culture rapid propagation method is proposed to obtain robust seedlings with a multiplication coefficient up to 5-10 times and a root length of 3-5 cm.
Abstract: The invention provides a tabebuia chrysantha tissue culture rapid propagation method. The method specifically comprises the following steps: (1) selection and disinfection of explants; (2) explants germination to obtain sterile test tube seedlings; (3) rapid propagation culture of the test tube seedlings to obtain cluster buds; (4) strong seedling culture of the cluster buds to obtain robust plants; (5) rooting culture of the robust plants to obtain intact rooted seedlings; and (6) seedling exercising and transplanting. By using the propagation method and a medium formula to perform tabebuia chrysantha tissue culture, the obtained single buds have a multiplication coefficient up to 5-10 times, the obtained tissue culture seedlings have a rooting rate of 95% or more, each plant has 3-4 roots in average, the root length is 3-5 cm, and the sand bed transplanting survival rate is 98% or more; and the tabebuia chrysantha tissue culture can be carried out quickly, conveniently and efficiently, and large-scale production of the tabebuia chrysantha tissue culture seedlings is achieved.
Patent•
24 Feb 2016
TL;DR: In this article, a dracaena cochinchinensis loose callus induction method was proposed, and the callus obtained through the method is peak green or milk white and loose and has a strong propagation capability.
Abstract: Provided is a dracaena cochinchinensis loose callus induction method. The method comprises the following steps: firstly, dracaena cochinchinensis seeds or buds are taken as explants, and disinfection is carried out; secondly, the explants after disinfection are placed in an MS basal medium, and buds or clustered buds are generated through induction; thirdly, the aseptic buds are placed in MS, B5, N6 media with 6-benzyladenine 6-BA and naphthylacetic acid NAA, and cultured, and callus is obtained; fourthly, the callus is placed in induction media with different hormones and cultured, and loose callus is obtained. The callus obtained through the method is peak green or milk white and loose and has a strong propagation capability. A lot of well-grown loose callus can be induced in short time, and materials are provided for production of dracaena cochinchinensis total alkaloids through cell suspension culture.