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Wei Xiaobing

Bio: Wei Xiaobing is an academic researcher. The author has contributed to research in topics: C-C chemokine receptor type 7 & Apoptosis. The author has an hindex of 2, co-authored 3 publications receiving 11 citations.

Papers
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Journal ArticleDOI
TL;DR: The data suggest that CCL19 acts as a chicken PWBC chemotactic factor and facilitate the infiltration of PWBC (especially T cells) into the bursae after IBDV infection.

8 citations

Journal ArticleDOI
TL;DR: The results showed that PCA could effectively alleviate bursal pathological changes at the early stage of IBDV invasion, and PCA as a potent antiviral drug precursor is expected to be applied in the poultry industry as a substitute for clinical antiviral application.

6 citations

Patent
28 Oct 2015
TL;DR: In this paper, a multi-RT-PCR method for monitoring pollution of six main viruses in a pig farm environment through one system and application is provided, which can be used for monitoring the pollution of the six viruses in air, fodder, drinking water, appliances, excrement and pig groups.
Abstract: The invention provides a multi-RT-PCR method for monitoring pollution of six main viruses in a pig farm environment through one system and application. According to the method, primer design is conducted for CSFV, JEV, PRRSV, PCV-2, PRV and PPV, a multi-RT-PCR reaction system for the six viruses is established, air samples are collected through liquid collision, the viruses are subjected to separation concentration through ultrafiltration centrifugation, and the method for detecting the pollution of the main viruses in the pig farm environment is established. The method can be used for monitoring the pollution of the six viruses in air, fodder, drinking water, appliances, excrement and pig groups in the pig house and pig farm environment, and technical support is provided for establishing a main virus pollution early-warning mechanism and a main virus disease preventing, controlling and purifying system in the pig farm environment.

2 citations


Cited by
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Journal ArticleDOI
TL;DR: This review aims to comprehensively summarize the pharmacology, pharmacokinetics, and toxicity of protocatechuic acid with emphasis on its pharmacological activities discovered in recent 5 years, so as to provide more up-to-date and thorough information for the preclinical and clinical research of protocatedchuic Acid in the future.

80 citations

Journal ArticleDOI
TL;DR: Dihydromyricetin could reduce ileum NLR family pyrin domain-containing 3 (NLRP3), caspase-1, interleukin (IL)-1β and IL-18 expression to protect tissues from pyroptosis and inhibited toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) signalling pathway activation triggered by Escherichia coli LPS.
Abstract: Lipopolysaccharide (LPS) as a major component of Escherichia coli cell wall can cause inflammation and cell death. Dihydromyricetin (ampelopsin, DHM) is a natural flavonoid compound with anti-inflammatory, anti-oxidant and anti-bacterial effects. The preventive effects of DHM against ileum injury remain unclear. Here, we explored the protective role of DHM against LPS-induced ileum injury in chickens. In this study, DHM significantly attenuated LPS-induced alteration in diamine oxidase, malondialdehyde, reduced glutathione, glutathione peroxidase and superoxide dismutase levels in chicken plasma and ileum. Histology evaluation showed that the structure of blood vessels in ileum was seriously fragmented and presence of necrotic tissue in the lumen in the LPS group. Scanning electron microscopic observation revealed that the surface of the villi was rough and uneven, the structure was chaotic, and the normal finger shape was lost in the LPS group. In contrast, 0.05% and 0.1% DHM treatment partially alleviated the abnormal morphology. Additionally, DHM maintained the barrier function by restoring the protein expression of occludin, claudin-1 and zonula occludens protein-1. DHM inhibited apoptosis through the reduction of the expression of bax and caspase-3 and restored the expression of bcl-2. Importantly, DHM could reduce ileum NLR family pyrin domain-containing 3 (NLRP3), caspase-1, interleukin (IL)-1β and IL-18 expression to protect tissues from pyroptosis and inhibited toll-like receptor 4 (TLR4)/nuclear factor kappa-B (NF-κB) signalling pathway. In summary, DHM attenuated the ileum mucosal damage, oxidative stress and apoptosis, maintained barrier function, inhibited NLRP3 inflammasome and TLR4/NF-κB signalling pathway activation triggered by Escherichia coli LPS.

24 citations

Journal ArticleDOI
TL;DR: In this paper, a review of NF-κB functioning in poultry with a specific emphasis on its nutritional modulation under various stress conditions is presented. But, the role of Nrf2 in poultry has not yet been characterized.
Abstract: Redox biology is a very quickly developing area of modern biological sciences, and roles of redox homeostasis in health and disease have recently received tremendous attention There are a range of redox pairs in the cells/tissues responsible for redox homeostasis maintenance/regulation In general, all redox elements are interconnected and regulated by various means, including antioxidant and vitagene networks The redox status is responsible for maintenance of cell signaling and cell stress adaptation Physiological roles of redox homeostasis maintenance in avian species, including poultry, have received limited attention and are poorly characterized However, for the last 5 years, this topic attracted much attention, and a range of publications covered some related aspects In fact, transcription factor Nrf2 was shown to be a master regulator of antioxidant defenses via activation of various vitagenes and other protective molecules to maintain redox homeostasis in cells/tissues It was shown that Nrf2 is closely related to another transcription factor, namely, NF-κB, responsible for control of inflammation; however, its roles in poultry have not yet been characterized Therefore, the aim of this review is to describe a current view on NF-κB functioning in poultry with a specific emphasis to its nutritional modulation under various stress conditions In particular, on the one hand, it has been shown that, in many stress conditions in poultry, NF-κB activation can lead to increased synthesis of proinflammatory cytokines leading to systemic inflammation On the other hand, there are a range of nutrients/supplements that can downregulate NF-κB and decrease the negative consequences of stress-related disturbances in redox homeostasis In general, vitagene-NF-κB interactions in relation to redox balance homeostasis, immunity, and gut health in poultry production await further research

21 citations

Journal ArticleDOI
TL;DR: High-throughput proteomic sequencing showed that chicken macrophage migration inhibitory factor (chMIF) was upregulated uniquely in primary bursal cells infected with vvIBDV compared with infection by nonpathogenic attenuated IBDV, and elucidates critical molecular determinants underlying vv IBDV-mediated initiation of acute inflammation.
Abstract: Infectious bursal disease (IBD) is one of the main threats to the poultry industry worldwide. In China, very virulent IBD virus (vvIBDV) is the main prevalent virus strain, causing inflammation, immunosuppression, and high mortality in young chickens. To determine whether this acute inflammation can trigger lesions or even death in chickens, it is important to study the mechanism of vvIBDV pathogenicity. Thus, in the current study, we investigated the inflammation response, bursal lesions, and mortality in chickens caused by vvIBDV at different time points postinfection. Results showed an upregulation of proinflammatory cytokines, including interleukin-1β and interleukin-18, and macrophage infiltration in bursa in response to vvIBDV infection. High-throughput proteomic sequencing based on isobaric tags for relative and absolute quantitation showed that chicken macrophage migration inhibitory factor (chMIF) was upregulated uniquely in primary bursal cells infected with vvIBDV compared with infection by nonpathogenic attenuated IBDV. We confirmed that chMIF was upregulated by vvIBDV infection both in vivo and in vitro. Moreover, chMIF was extracellularly secreted by infected DT40 and primary bursal cells. Further experiments revealed that the secreted chMIF could induce migration of peripheral blood mononuclear cells and promote transcription of proinflammatory cytokines in chicken primary macrophages. Notably, these effects of chMIF could be reduced by using an MIF specific inhibitor. Thus, our study elucidates critical molecular determinants underlying vvIBDV-mediated initiation of acute inflammation, which might be pivotal to understand the mechanism of vvIBDV pathogenicity.

13 citations

Journal ArticleDOI
TL;DR: Vaxsafe MG (strain ts-304) is a new vaccine candidate that is efficacious at a lower dose than the current commercial vaccine strain ts-11, from which it is derived and appears to protect the structural and functional integrity of the tracheal mucosa 2 weeks after infection with M. gallisepticum.
Abstract: Mycoplasma gallisepticum is the primary etiological agent of chronic respiratory disease in chickens. Live attenuated vaccines are most commonly used in the field to control the disease, but current vaccines have some limitations. Vaxsafe MG (strain ts-304) is a new vaccine candidate that is efficacious at a lower dose than the current commercial vaccine strain ts-11, from which it is derived. In this study, the transcriptional profiles of the trachea of unvaccinated chickens and chickens vaccinated with strain ts-304 were compared 2 weeks after challenge with M. gallisepticum strain Ap3AS during the chronic stage of infection. After challenge, genes, gene ontologies, pathways, and protein classes involved in inflammation, cytokine production and signaling, and cell proliferation were upregulated, while those involved in formation and motor movement of cilia, formation of intercellular junctional complexes, and formation of the cytoskeleton were downregulated in the unvaccinated birds compared to the vaccinated birds, reflecting immune dysregulation and the pathological changes induced in the trachea by infection with M. gallisepticum Vaccination appears to protect the structural and functional integrity of the tracheal mucosa 2 weeks after infection with M. gallisepticum.

12 citations