William K. Vencill

Bio: William K. Vencill is an academic researcher from University of Georgia. The author has contributed to research in topics: Amaranthus palmeri & Imazapic. The author has an hindex of 21, co-authored 73 publications receiving 2320 citations. Previous affiliations of William K. Vencill include Colorado State University.

Gene amplification confers glyphosate resistance in Amaranthus palmeri

Abstract: The herbicide glyphosate became widely used in the United States and other parts of the world after the commercialization of glyphosate-resistant crops. These crops have constitutive overexpression of a glyphosate-insensitive form of the herbicide target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Increased use of glyphosate over multiple years imposes selective genetic pressure on weed populations. We investigated recently discovered glyphosate-resistant Amaranthus palmeri populations from Georgia, in comparison with normally sensitive populations. EPSPS enzyme activity from resistant and susceptible plants was equally inhibited by glyphosate, which led us to use quantitative PCR to measure relative copy numbers of the EPSPS gene. Genomes of resistant plants contained from 5-fold to more than 160-fold more copies of the EPSPS gene than did genomes of susceptible plants. Quantitative RT-PCR on cDNA revealed that EPSPS expression was positively correlated with genomic EPSPS relative copy number. Immunoblot analyses showed that increased EPSPS protein level also correlated with EPSPS genomic copy number. EPSPS gene amplification was heritable, correlated with resistance in pseudo-F2 populations, and is proposed to be the molecular basis of glyphosate resistance. FISH revealed that EPSPS genes were present on every chromosome and, therefore, gene amplification was likely not caused by unequal chromosome crossing over. This occurrence of gene amplification as an herbicide resistance mechanism in a naturally occurring weed population is particularly significant because it could threaten the sustainable use of glyphosate-resistant crop technology.

Glyphosate-resistant Palmer amaranth (Amaranthus palmeri ) confirmed in Georgia

Abstract: A glyphosate-resistant Palmer amaranth biotype was confirmed in central Georgia. In the field, glyphosate applied to 5- to 13-cm-tall Palmer amaranth at three times the normal use rate of 0.84 kg ae ha−1 controlled this biotype only 17%. The biotype was controlled 82% by glyphosate at 12 times the normal use rate. In the greenhouse, I50 values (rate necessary for 50% inhibition) for visual control and shoot fresh weight, expressed as percentage of the nontreated, were 8 and 6.2 times greater, respectively, with the resistant biotype compared with a known glyphosate-susceptible biotype. Glyphosate absorption and translocation and the number of chromosomes did not differ between biotypes. Shikimate was detected in leaf tissue of the susceptible biotype treated with glyphosate but not in the resistant biotype. Nomenclature: Glyphosate; Palmer amaranth, Amaranthus palmeri S. Wats; AMAPA.

Herbicide Resistance: Toward an Understanding of Resistance Development and the Impact of Herbicide-Resistant Crops

Abstract: This is the publisher’s final pdf. The published article is copyrighted by the Weed Science Society of America and can be found at: http://wssajournals.org/loi/wees. To the best of our knowledge, one or more authors of this paper were federal employees when contributing to this work.

Establishing the Geographical Distribution and Level of Acetolactate Synthase Resistance of Palmer Amaranth (Amaranthus palmeri) Accessions in Georgia

Abstract: Palmer amaranth resistance to acetolactate synthase (ALS)–inhibiting herbicides was first identified in Georgia in 2000. Since then, complaints from peanut producers have increased concerning failure of ALS herbicides in controlling Palmer amaranth. Because efficacy of ALS herbicides can be compromised under adverse conditions, seeds from Palmer amaranth plants that escaped weed control were collected across the peanut-growing region in Georgia to investigate the cause of these reported failures. Greenhouse and growth-chamber studies were conducted using these seeds to evaluate whether weed escapes were a result of Palmer amaranth resistance to ALS herbicides. Each of the 61 accessions collected across Georgia exhibited varying levels of resistance to imazapic applied POST (< 55% control, relative to ALS-susceptible Palmer amaranth). Subsamples of the accessions were evaluated for their response to imazapic rates, which indicated variable levels of resistance across Palmer amaranth accessions. Th...

Impact of conservation tillage and nutrient management on soil water and yield of cotton fertilized with poultry litter or ammonium nitrate in the Georgia Piedmont

Abstract: Cotton has become a dominant crop in the southeastern USA, but only about 12% of the 620,000 ha of cotton ( Gossipium hirsutum L.) in Georgia, for example, is under conservation tillage. Georgia and bordering states produce about 42% of the poultry in the United States and in Georgia alone, this results in over 1.6 million Mg of poultry litter (PL) annually. The fertilizer value of PL is well-recognized but much of it is applied to pastures and only a small percentage is applied to crop land. Limited information is available on the response of cotton to PL as fertilizer in conservation tillage systems in the Southeast. The performance of cotton under two tillage and two fertilizer treatments was evaluated from 1996 to 1999 to highlight management options for increased adoption of conservation tillage and PL use. Cotton, followed by a rye ( Secale cereale L.) cover crop, was grown under a factorial arrangement of tillage (no-till (NT) vs conventional tillage (CT)) and fertilizer (ammonium nitrate, as conventional fertilizer (CF) vs PL) on a Cecil sandy loam (clayey, kaolinitic thermic Typic Kanhapludult; Chromi-Alumic Acrisol) near Watkinsville, Georgia. Average lint yield from 1996 to 1999 was in the sequence no - till poultry litter ( NTPL )> no - till conventional fertilizer ( NTCF )> conventional tillage poultry litter ( CTPL )> conventional tillage and fertilizer ( CTCF ) . Differences were significant at P ≤0.05 for NTPL vs CTPL, NTPL vs CTCF, and NTCF vs CTCF. Average yield differences were also significant between NT and CT but not PL and CF. PL yielded more than CF only in 1997. NT generally had a more favorable soil water regime than CT. Yield differences among treatments occurred during the first 3 years only. Drought in the fourth year reduced yield across all treatments and negated treatment effects. Lint yield would increase in the southeastern USA and an additional outlet for the PL would be created by adopting NT and fertilizing with PL in cotton production.

A User’s Guide

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Evolution in Action: Plants Resistant to Herbicides

Abstract: Modern herbicides make major contributions to global food production by easily removing weeds and substituting for destructive soil cultivation. However, persistent herbicide selection of huge weed numbers across vast areas can result in the rapid evolution of herbicide resistance. Herbicides target specific enzymes, and mutations are selected that confer resistance-endowing amino acid substitutions, decreasing herbicide binding. Where herbicides bind within an enzyme catalytic site very few mutations give resistance while conserving enzyme functionality. Where herbicides bind away from a catalytic site many resistance-endowing mutations may evolve. Increasingly, resistance evolves due to mechanisms limiting herbicide reaching target sites. Especially threatening are herbicide-degrading cytochrome P450 enzymes able to detoxify existing, new, and even herbicides yet to be discovered. Global weed species are accumulating resistance mechanisms, displaying multiple resistance across many herbicides and posing a great challenge to herbicide sustainability in world agriculture. Fascinating genetic issues associated with resistance evolution remain to be investigated, especially the possibility of herbicide stress unleashing epigenetic gene expression. Understanding resistance and building sustainable solutions to herbicide resistance evolution are necessary and worthy challenges.

Reducing the Risks of Herbicide Resistance: Best Management Practices and Recommendations

Abstract: Herbicides are the foundation of weed control in commercial crop-production systems. However, herbicide-resistant (HR) weed populations are evolving rapidly as a natural response to selection pressure imposed by modern agricultural management activities. Mitigating the evolution of herbicide resistance depends on reducing selection through diversification of weed control techniques, minimizing the spread of resistance genes and genotypes via pollen or propagule dispersal, and eliminating additions of weed seed to the soil seedbank. Effective deployment of such a multifaceted approach will require shifting from the current concept of basing weed management on single-year economic thresholds.

Concerning RNA-guided gene drives for the alteration of wild populations

Abstract: Gene drives may be capable of addressing ecological problems by altering entire populations of wild organisms, but their use has remained largely theoretical due to technical constraints. Here we consider the potential for RNA-guided gene drives based on the CRISPR nuclease Cas9 to serve as a general method for spreading altered traits through wild populations over many generations. We detail likely capabilities, discuss limitations, and provide novel precautionary strategies to control the spread of gene drives and reverse genomic changes. The ability to edit populations of sexual species would offer substantial benefits to humanity and the environment. For example, RNA-guided gene drives could potentially prevent the spread of disease, support agriculture by reversing pesticide and herbicide resistance in insects and weeds, and control damaging invasive species. However, the possibility of unwanted ecological effects and near-certainty of spread across political borders demand careful assessment of each potential application. We call for thoughtful, inclusive, and well-informed public discussions to explore the responsible use of this currently theoretical technology.

Gene amplification confers glyphosate resistance in Amaranthus palmeri

Abstract: The herbicide glyphosate became widely used in the United States and other parts of the world after the commercialization of glyphosate-resistant crops. These crops have constitutive overexpression of a glyphosate-insensitive form of the herbicide target site gene, 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS). Increased use of glyphosate over multiple years imposes selective genetic pressure on weed populations. We investigated recently discovered glyphosate-resistant Amaranthus palmeri populations from Georgia, in comparison with normally sensitive populations. EPSPS enzyme activity from resistant and susceptible plants was equally inhibited by glyphosate, which led us to use quantitative PCR to measure relative copy numbers of the EPSPS gene. Genomes of resistant plants contained from 5-fold to more than 160-fold more copies of the EPSPS gene than did genomes of susceptible plants. Quantitative RT-PCR on cDNA revealed that EPSPS expression was positively correlated with genomic EPSPS relative copy number. Immunoblot analyses showed that increased EPSPS protein level also correlated with EPSPS genomic copy number. EPSPS gene amplification was heritable, correlated with resistance in pseudo-F2 populations, and is proposed to be the molecular basis of glyphosate resistance. FISH revealed that EPSPS genes were present on every chromosome and, therefore, gene amplification was likely not caused by unequal chromosome crossing over. This occurrence of gene amplification as an herbicide resistance mechanism in a naturally occurring weed population is particularly significant because it could threaten the sustainable use of glyphosate-resistant crop technology.