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Winston X. Yan
Researcher at Broad Institute
Publications - 50
Citations - 14070
Winston X. Yan is an academic researcher from Broad Institute. The author has contributed to research in topics: CRISPR & Genome editing. The author has an hindex of 26, co-authored 47 publications receiving 11013 citations. Previous affiliations of Winston X. Yan include Harvard University & Rutgers University.
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Journal ArticleDOI
Rationally Engineered Cas9 Nucleases With Improved Specificity
TL;DR: In this paper, the authors used structure-guided protein engineering to improve the specificity of Streptococcus pyogenes Cas9 (SpCas9) using targeted deep sequencing and unbiased whole-genome off-target analysis to assess Cas9-mediated DNA cleavage in human cells.
In vivo genome editing using Staphylococcus aureus Cas9
F. Ann Ran,Le Cong,Winston X. Yan,David A. Scott,Jonathan S. Gootenberg,Andrea J. Kriz,Bernd Zetsche,Ophir Shalem,Xuebing Wu,Kira S. Makarova,Eugene V. Koonin,Phillip A. Sharp,Feng Zhang +12 more
TL;DR: In this paper, the RNA-guided endonuclease Cas9 has emerged as a versatile genome-editing platform and has been used for basic research and therapeutic applications that use the highly versatile adeno-associated virus (AAV) delivery vehicle.
Journal ArticleDOI
In vivo genome editing using Staphylococcus aureus Cas9
F. Ann Ran,Le Cong,Winston X. Yan,David A. Scott,Jonathan S. Gootenberg,Andrea J. Kriz,Bernd Zetsche,Ophir Shalem,Xuebing Wu,Kira S. Makarova,Eugene V. Koonin,Phillip A. Sharp,Feng Zhang +12 more
TL;DR: Six smaller Cas9 orthologues are characterized and it is shown that Cas9 from Staphylococcus aureus (SaCas9) can edit the genome with efficiencies similar to those of SpCas9, while being more than 1 kilobase shorter.
Journal ArticleDOI
Evolutionary classification of CRISPR-Cas systems: a burst of class 2 and derived variants.
Kira S. Makarova,Yuri I. Wolf,Jaime Iranzo,Sergey Shmakov,Omer S. Alkhnbashi,Stan J. J. Brouns,Emmanuelle Charpentier,David R. Cheng,Daniel H. Haft,Philippe Horvath,Sylvain Moineau,Francisco J. M. Mojica,David Scott,Shiraz A. Shah,Virginijus Siksnys,Michael P. Terns,Česlovas Venclovas,Malcolm F. White,Alexander F. Yakunin,Alexander F. Yakunin,Winston X. Yan,Feng Zhang,Roger A. Garrett,Rolf Backofen,John van der Oost,Rodolphe Barrangou,Eugene V. Koonin +26 more
TL;DR: An updated evolutionary classification of CRISPR–Cas systems and cas genes is provided, with an emphasis on the major developments that have occurred since the publication of the latest classification, in 2015, which includes 2 classes, 6 types and 33 subtypes.
Journal ArticleDOI
In vivo genome editing improves muscle function in a mouse model of Duchenne muscular dystrophy.
Christopher E. Nelson,Chady H. Hakim,David G. Ousterout,Pratiksha I. Thakore,Eirik A. Moreb,Ruth M. Castellanos Rivera,Sarina Madhavan,Xiufang Pan,F. Ann Ran,F. Ann Ran,Winston X. Yan,Winston X. Yan,Winston X. Yan,Aravind Asokan,Feng Zhang,Dongsheng Duan,Charles A. Gersbach +16 more
TL;DR: In this paper, an adeno-associated virus was used to deliver the clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system to the mdx mouse model of Duchenne muscular dystrophy (DMD) to remove the mutated exon 23 from the dystrophin gene.