Won Do Heo

TL;DR: This study suggests that STIM proteins function as Ca(2+) store sensors in the signaling pathway connecting Ca(1+)-store-depletion-mediated Ca( 2+) influx, and suggests that this mutant failed to respond to store depletion.

TL;DR: This work surveyed PM-targeting mechanisms by imaging the subcellular localization of 125 fluorescent protein–conjugated Ras, Rab, Arf, and Rho proteins and found that proteins with polybasic clusters dissociated from the PM only when both PI(4,5)P2 and phosphatidylinositol 3,4, 5-trisphosphate were depleted.

TL;DR: STIM (stromal interaction molecule) 1 and STIM2 stood Ca 2+ signaling in nonexcitable cells is typically initiated out in their ability to suppress the sustainedCa 2+ sig- by receptor-triggered production of inositol-1,4,5-tris- nals while showing little effect on the peak amplitude.

TL;DR: Using molecular engineering of different targeting and enzymatic fusion constructs and a new rapamycin analog, Rho GTPases were directly activated or inactivated on a timescale of seconds, which was followed by pronounced cell morphological changes.

TL;DR: A new tool for intracellular delivery of target proteins, named ‘exosomes for protein loading via optically reversible protein–protein interactions’ (EXPLORs), which is able to successfully load cargo proteins into newly generated exosomes using optogenetic control of protein-protein interactions between the cargo and an exosome-localized partner.